Dr Ruby Peters

School of Mathematical and Physical Sciences

Physics of Life Early Career Fellow

r.peters@sheffield.ac.uk

F23, Hicks Building

Full contact details

Dr Ruby Peters
School of Mathematical and Physical Sciences
F23
Hicks Building
Hounsfield Road
Sheffield
S3 7RH

Profile

I am an early career fellow in the physics of life. My research group investigates how the structural, mechanical and material properties of actin cytoskeletal networks drive key cell functions. Since a tight regulation of cellular mechanics is critical to various physiological processes, and a deregulation of actin network architecture and dynamics is a hallmark of disease, there is an urgent need to uncover how actin networks control the mechanical properties of cells.

To address this, my group develops advanced fluorescence microscopy and computational approaches to quantify the nanoscale organisation and dynamics of the actin cytoskeleton. In combination, we use state-of-the-art atomic force microscopy to map the biophysical properties of the cell surface. By integrating these techniques, we gain unprecedented insights into how actin networks mechanically function at both the nano and cellular scales, and how this in turn governs cellular behaviour.

Alongside my core research, I am passionate about improving equality, diversity and inclusion within STEM.

Publications

Show: Featured publications All publications

Featured publications

Journal articles

Zhang B, Daly S, Zhu C, Lenz MO, Weiss LE, Needham L-M, Peters R, Lee SF & O’Holleran K (2024) Vortex light field microscopy: 3D spectral single-molecule imaging with a twist. Optica, 11(11), 1519-1519.
Bruggeman E, Zhang O, Needham L-M, Körbel M, Daly S, Cheetham M, Peters R, Wu T, Klymchenko AS, Davis SJ , Paluch EK et al (2024) POLCAM: instant molecular orientation microscopy for the life sciences. Nature Methods, 21, 1873-1883. View this article in WRRO
Yanagida A, Corujo-Simon E, Revell CK, Sahu P, Stirparo GG, Aspalter IM, Winkel AK, Peters R, De Belly H, Cassani DAD , Achouri S et al (2022) Cell surface fluctuations regulate early embryonic lineage sorting. Cell, 185(5), 777-793.e20.
Peters R, Griffié J, Williamson DJ, Aaron J, Khuon S & Owen DM (2019) Development of 2-colour and 3D SMLM data analysis methods for fibrous spatial point patterns. Journal of Physics D: Applied Physics, 52(1), 014005-014005.
Peters R, Benthem Muñiz M, Griffié J, Williamson DJ, Ashdown GW, Lorenz CD & Owen DM (2017) Quantification of fibrous spatial point patterns from single-molecule localization microscopy (SMLM) data. Bioinformatics, 33(11), 1703-1711.
Peters R, Sandiford L, Owen DM, Kemal E, Bourke S, Dailey LA & Green M (2016) Red-emitting protein-coated conjugated polymer nanoparticles. Photochemical & Photobiological Sciences, 15(11), 1448-1452.
Daly S, Ferreira Fernandes J, Bruggeman E, Handa A, Peters R, Benaissa S, Zhang B, Beckwith JS, Sanders EW, Sims RR , Klenerman D et al () High-density volumetric super-resolution microscopy. Nature Communications, 15(1).
Truong Quang BA, Peters R, Cassani DAD, Chugh P, Clark AG, Agnew M, Charras G & Paluch EK () Extent of myosin penetration within the actin cortex regulates cell surface mechanics. Nature Communications, 12(1).
Williamson DJ, Burn GL, Simoncelli S, Griffié J, Peters R, Davis DM & Owen DM () Machine learning for cluster analysis of localization microscopy data. Nature Communications, 11(1).
Griffié J, Peters R & Owen DM () An agent-based model of molecular aggregation at the cell membrane. PLOS ONE, 15(2), e0226825-e0226825.
Peters R, Griffié J, Burn GL, Williamson DJ & Owen DM () Quantitative fibre analysis of single-molecule localization microscopy data. Scientific Reports, 8(1).
Griffié J, Shlomovich L, Williamson DJ, Shannon M, Aaron J, Khuon S, L. Burn G, Boelen L, Peters R, Cope AP , Cohen EAK et al () 3D Bayesian cluster analysis of super-resolution data reveals LAT recruitment to the T cell synapse. Scientific Reports, 7(1).

All publications

Journal articles

Zhang B, Daly S, Zhu C, Lenz MO, Weiss LE, Needham L-M, Peters R, Lee SF & O’Holleran K (2024) Vortex light field microscopy: 3D spectral single-molecule imaging with a twist. Optica, 11(11), 1519-1519.
Bruggeman E, Zhang O, Needham L-M, Körbel M, Daly S, Cheetham M, Peters R, Wu T, Klymchenko AS, Davis SJ , Paluch EK et al (2024) POLCAM: instant molecular orientation microscopy for the life sciences. Nature Methods, 21, 1873-1883. View this article in WRRO
Yanagida A, Corujo-Simon E, Revell CK, Sahu P, Stirparo GG, Aspalter IM, Winkel AK, Peters R, De Belly H, Cassani DAD , Achouri S et al (2022) Cell surface fluctuations regulate early embryonic lineage sorting. Cell, 185(5), 777-793.e20.
Peters R, Griffié J, Williamson DJ, Aaron J, Khuon S & Owen DM (2019) Development of 2-colour and 3D SMLM data analysis methods for fibrous spatial point patterns. Journal of Physics D: Applied Physics, 52(1), 014005-014005.
Kemal E, Peters R, Bourke S, Fairclough S, Bergstrom-Mann P, Owen DM, Sandiford L, Dailey LA & Green M (2018) Magnetic conjugated polymer nanoparticles doped with a europium complex for biomedical imaging. Photochemical & Photobiological Sciences, 17(6), 718-721.
Griffié J, Burn GL, Williamson DJ, Peters R, Rubin‐Delanchy P & Owen DM (2018) Dynamic Bayesian Cluster Analysis of Live‐Cell Single Molecule Localization Microscopy Datasets. Small Methods, 2(9).
Ashdown GW, Burn GL, Williamson DJ, Pandžić E, Peters R, Holden M, Ewers H, Shao L, Wiseman PW & Owen DM (2017) Live-Cell Super-resolution Reveals F-Actin and Plasma Membrane Dynamics at the T Cell Synapse. Biophysical Journal, 112(8), 1703-1713.
Peters R, Benthem Muñiz M, Griffié J, Williamson DJ, Ashdown GW, Lorenz CD & Owen DM (2017) Quantification of fibrous spatial point patterns from single-molecule localization microscopy (SMLM) data. Bioinformatics, 33(11), 1703-1711.
Peters R, Sandiford L, Owen DM, Kemal E, Bourke S, Dailey LA & Green M (2016) Red-emitting protein-coated conjugated polymer nanoparticles. Photochemical & Photobiological Sciences, 15(11), 1448-1452.
Daly S, Ferreira Fernandes J, Bruggeman E, Handa A, Peters R, Benaissa S, Zhang B, Beckwith JS, Sanders EW, Sims RR , Klenerman D et al () High-density volumetric super-resolution microscopy. Nature Communications, 15(1).
Truong Quang BA, Peters R, Cassani DAD, Chugh P, Clark AG, Agnew M, Charras G & Paluch EK () Extent of myosin penetration within the actin cortex regulates cell surface mechanics. Nature Communications, 12(1).
Manzano-Núñez F, Peters R, Burks DJ & Noon LA () A High-Throughput In Situ Method for Estimation of Hepatocyte Nuclear Ploidy in Mice. Journal of Visualized Experiments(158).
Williamson DJ, Burn GL, Simoncelli S, Griffié J, Peters R, Davis DM & Owen DM () Machine learning for cluster analysis of localization microscopy data. Nature Communications, 11(1).
Griffié J, Peters R & Owen DM () An agent-based model of molecular aggregation at the cell membrane. PLOS ONE, 15(2), e0226825-e0226825.
Peters R, Griffié J, Burn GL, Williamson DJ & Owen DM () Quantitative fibre analysis of single-molecule localization microscopy data. Scientific Reports, 8(1).
Griffié J, Shlomovich L, Williamson DJ, Shannon M, Aaron J, Khuon S, L. Burn G, Boelen L, Peters R, Cope AP , Cohen EAK et al () 3D Bayesian cluster analysis of super-resolution data reveals LAT recruitment to the T cell synapse. Scientific Reports, 7(1).
Kemal E, Abelha TF, Urbano L, Peters R, Owen DM, Howes P, Green M & Dailey LA () Bright, near infrared emitting PLGA–PEG dye-doped CN-PPV nanoparticles for imaging applications. RSC Advances, 7(25), 15255-15264.

Conference proceedings papers

Peters R, Owen D & Griffie J (2017) Analysis of Fibrous Spatial Point Patterns from Single-Molecule Super-Resolution Microscopy Data. Biophysical Journal, Vol. 112(3) (pp 142a-142a)

Preprints

Shirgill S, Nieves DJ, Pike JA, Ahmed MA, Baragilly MHH, Savoye K, Worboys J, Hazime KS, Garcia A, Williamson DJ , Rubin-Delanchy P et al (2024) Nano-org, a functional resource for single-molecule localisation microscopy data, Cold Spring Harbor Laboratory.
Zhang B, Daly S, Zhu C, Lenz MO, Weiss LE, Needham L-M, Peters R, Lee SF & O’Holleran K (2024) Vortex light field microscopy: 3D spectral single-molecule imaging with a twist, Cold Spring Harbor Laboratory.
Daly S, Fernandes JF, Bruggeman E, Handa A, Peters R, Benaissa S, Zhang B, Beckwith JS, Sanders EW, Sims RR , Klenerman D et al (2023) High-density volumetric super-resolution microscopy, Cold Spring Harbor Laboratory.
Bruggeman E, Zhang O, Needham L-M, Körbel M, Daly S, Cheetham M, Peters R, Wu T, Klymchenko AS, Davis SJ , Paluch EK et al (2023) POLCAM: Instant molecular orientation microscopy for the life sciences, Cold Spring Harbor Laboratory.
Baragilly M, Nieves DJ, Williamson DJ, Peters R & Owen DM (2022) Measuring the similarity of SMLM-derived point-clouds, Cold Spring Harbor Laboratory.
Yanagida A, Revell C, Stirparo GG, Corujo-Simon E, Aspalter IM, Peters R, De Belly H, Cassani DAD, Achouri S, Blumenfeld R , Franze K et al (2020) Cell surface fluctuations regulate early embryonic lineage sorting, Cold Spring Harbor Laboratory.

Research group: Materials and Biological Physics Group