A team of 36 researchers and technicians from across the University, led by the Centre for Single Molecule Biology, has been awarded £305,000 in the recently announced BBSRC ALERT 2024 program. This funding will bring a groundbreaking new approach, known as Mass Photometry, to the University.
Mass Photometry is a state-of-the-art, single-molecule technique that determines the size of molecular assemblies based on light scattering. Understanding how biomolecules, such as proteins, lipids, nucleic acids, or carbohydrates, interact is essential for comprehending all biological processes. Sheffield researchers study a wide range of interactions, from simple protein-DNA binding to complex plant photosynthesis. These processes invariably involve changes in the size or mass of the biomolecules involved.
While observing mass changes in biomolecular assemblies is crucial, traditional laboratory methods can be time-consuming and technically challenging. Mass Photometry offers a universal solution, as all molecules scatter light regardless of their composition.
Although Sheffield researchers have been using Mass Photometry for various projects, access to this equipment often required travel to other UK locations. The BBSRC's strategic investment will now fund the installation of the latest generation Refeyn TwoMP Mass Photometer with MassFluidiX in the School of Biosciences. This instrumentation will be housed in the newly refurbished Biomolecular Interaction Suite in Firth Court.
This significant development will provide Sheffield researchers with cutting-edge technology to advance their understanding of fundamental biological processes and drive innovation in the field.