Professor Jon R Sayers
FRSB
Clinical Medicine, School of Medicine and Population Health
Professor of Functional Genomics
+44 114 215 9552
Full contact details
Clinical Medicine, School of Medicine and Population Health
L Floor
The Medical School
Beech Hill Road
Sheffield
S10 2RX
- Profile
-
For enquiries please contact - SMPH-West-Operational@sheffield.ac.uk
I teach graduate classes in biochemistry, protein/DNA sequence analysis, protein engineering of novel therapeutics and structure-based drug discovery.
I studied Chemistry (University of Birmingham, B.Sc. 1983) where I went on to gain a Ph.D. (1986) in the Nucleic Acids Group. Thesis title; Synthesis of Nucleoside Analogues. In 1986 I joined Fritz Eckstein’s group (Max-Planck-Institute for Experimental Medicine, Goettingen, to study restriction endonuclease-DNA interactions and helped develop a commercial system for site-directed mutagenesis (the 'phosphorothioate approach').
I also became interested in flap endonucleases, or 5'-3' exonucleases. In 1991 I moved to a Lectureship in the Biochemistry Department at the University of Wales, Bangor, UK, where I established a research group working on various aspects of molecular recognition. I moved to Sheffield in 1995, was promoted to Senior Lecturer in 1997, to a Readership in 1999 and a personal chair in 2006.
I have developed an interest in proteins secreted by Gram negative bacterial pathogens and how they interact with the host. We use a wide range of molecular and cell biological and biophysical techniques to unravel the detail of such interactions aided by close collaboration with structural biologists in Sheffield and the United States. I was elected as a Fellow of the Royal Society of Biology in 2013.
In 2001 I become a director and co-founder of Asterion Ltd., a biotech spin-off company (see Asterion Ltd.) and founded DeFENition Ltd in 2016 to develop inhibitors of essential bacterial enzymes as candidate antimicrobial agents. The latter builds on over thirty years of experience in mechanistic enzymology of flap endonucleases.
I have written an article for "The Biochemist" describing the trials and tribulations of an academic involved in the setting up of a spin-out company. It can be found under the Publications, All publications tab below.
COVID-19
My laboratory responded to the Covid-19 pandemic by manufacturing large quantities of SARS-CoV-2 Nucleocapsid protein (both native and His-tagged) for use in diagnostics. We have made these proteins cheaply available via the University's website.
- Research interests
-
The group is involved in four main areas:
- Structure-based drug design to generate new antimicrobial agents
- Molecular mechanisms involved in protein:DNA interactions
- Microbial proteases, pathogenesis and the host response
- Biotechnology for biomedical research and drug development
Ongoing topics include the following:
Mechanistic Studies on 5'-3' Exonucleases
A novel helical arch discovered in a 5'-3' exonuclease could explain how this class of essential replicative enzymes digest their nucleic acids substrates by a DNA threading mechanism. These enzymes are involved in replication of the lagging strand during DNA synthesis and in repair of DNA damage. Some members possess structure-specific endonuclease activity as well as exonuclease and RNase H activities. We have shown that a member of this important class of enzymes contains a novel DNA-binding motif, the helical arch (see above picture of the T5 exonuclease). We are using site-directed mutagenesis, crystallography and kinetic studies to determine how these complicated enzymes function. We have cloned and expressed a number of exonucleases from bacteria including the human pathogen Haemophilus influenzae. Early mechanistic work has been funded by the BBSRC, The Wellcome Trust. Translational research in this area has been funded by Innovate UK, The Department of Health and Social Welfare and DeFENition Ltd.
Protein-DNA Recognition
Many important biological processes such as gene expression are regulated by proteins binding to specific DNA sequences. We are studying novel DNA-binding proteins from viruses and pathogenic bacteria. We have chosen proteins with no sequence homologues in the databases. Such proteins are thus unique and studying how they recognize their target sequences should provide new insights into molecular recognition processes.
Significance of IgA1 Proteases in Pathogenic Neisseria meningitidis and Haemophilus influenzae
We discovered a strong link between high levels of IgA1 protease production and pathogenesis in Neisseria and Haemophilus two meningitis-causing organisms. The protease attacks IgA1, a major component of the mucosal immune system. This work has been funded by The Meningitis Research Foundation, Colin Beattie Memorial Fund and the Medical Research Council.
Developing inhibitors of flap endonucleases to combat microbial infection
We are carrying out hit-to-lead programmes targeting the Flap EndoNucleases (FENs), members of the 5'-3' / 5' nuclease family which process the branched DNA structures (5′ flaps) arising during DNA replication. FENs are found as independent globular proteins in eukaryotes including parasites, and as part of DNA polymerase I in bacteria. Knockouts of genes encoding FEN enzymes have proved lethal in all organisms tested to date. We use a combination of structural studies (X ray and NMR), in silico screening and structure-based drug design to develop specific inhibitors which may one day be developed into new drugs for treating bacterial and parasite infections.
- Publications
-
Show: Featured publications All publications
Featured publications
Journal articles
- Efficient overexpression and purification of severe acute respiratory syndrome coronavirus 2 nucleocapsid proteins in Escherichia coli. Biochemical Journal, 481(11), 669-682. View this article in WRRO
- Bacteriophage T5 gene D10 encodes a branch-migration protein. Scientific Reports, 6. View this article in WRRO
- A long-acting GH receptor antagonist through fusion to GH binding protein.. Scientific Reports, 6. View this article in WRRO
- Direct observation of DNA threading in flap endonuclease complexes. Nature Structural and Molecular Biology, 23, 640-646. View this article in WRRO
- Neutralizing mutations of carboxylates that bind metal 2 in T5 flap endonuclease result in an enzyme that still requires two metal ions.. J Biol Chem, 286(35), 30878-30887.
- Immunogenicity, toxicology, pharmacokinetics and pharmacodynamics of growth hormone ligand-receptor fusions.. Clin Sci (Lond), 119(11), 483-491.
- Active site substitutions delineate distinct classes of eubacterial flap endonuclease.. Biochem J, 418(2), 285-292. View this article in WRRO
- Investigating the interaction between osteoprotegerin and receptor activator of NF-kappaB or tumor necrosis factor-related apoptosis-inducing ligand: evidence for a pivotal role for osteoprotegerin in regulating two distinct pathways.. J Biol Chem, 282(43), 31601-31609.
- A ligand-receptor fusion of growth hormone forms a dimer and is a potent long-acting agonist.. Nat Med, 13(9), 1108-1113.
- Molecular interactions of Escherichia coli ExoIX and identification of its associated 3'-5' exonuclease activity.. Nucleic Acids Res, 35(12), 4094-4102. View this article in WRRO
- Schistosoma mansoni secretes a chemokine binding protein with antiinflammatory activity. J EXP MED, 202(10), 1319-1325. View this article in WRRO
- Roles of divalent metal ions in flap endonuclease-substrate interactions.. Nat Struct Mol Biol, 11(5), 450-456.
- Dynamic evidence for metal ion catalysis in the reaction mediated by a flap endonuclease.. EMBO J, 22(5), 995-1004.
- Interactions of mutant and wild-type flap endonucleases with oligonucleotide substrates suggest an alternative model of DNA binding.. Proc Natl Acad Sci U S A, 99(13), 8542-8547.
- Nontypeable Haemophilus influenzae in carriage and disease: a difference in IgA1 protease activity levels.. JAMA, 287(13), 1699-1705.
- Mutagenesis of conserved lysine residues in bacteriophage T5 5'-3' exonuclease suggests separate mechanisms of endo-and exonucleolytic cleavage.. Proc Natl Acad Sci U S A, 96(1), 38-43.
- Bacteriophage collagen. SCIENCE, 279(5358), 1834-1834.
- A helical arch allowing single-stranded DNA to thread through T5 5'-exonuclease. NATURE, 382(6586), 90-93.
All publications
Journal articles
- Efficient overexpression and purification of severe acute respiratory syndrome coronavirus 2 nucleocapsid proteins in Escherichia coli. Biochemical Journal, 481(11), 669-682. View this article in WRRO
- Establishing SARS-CoV-2 Membrane protein-specific antibodies as a valuable serological target via high-content microscopy. iScience, 107056-107056.
- Risk factors for SARS-CoV-2 seroprevalence following the first pandemic wave in UK healthcare workers in a large NHS Foundation Trust. Wellcome Open Research, 6, 220.
- Low-dose methotrexate in myeloproliferative neoplasm models.. Haematologica, 102, e336-e339. View this article in WRRO
- Schistosoma mansoni cercarial elastase (SmCE): differences in immunogenic properties of native and recombinant forms. Parasitology. View this article in WRRO
- Bacteriophage T5 gene D10 encodes a branch-migration protein. Scientific Reports, 6. View this article in WRRO
- A long-acting GH receptor antagonist through fusion to GH binding protein.. Scientific Reports, 6. View this article in WRRO
- Direct observation of DNA threading in flap endonuclease complexes. Nature Structural and Molecular Biology, 23, 640-646. View this article in WRRO
- OR5-3: A GH antagonist fusion with GH binding protein is biologically active, shows delayed clearance and inhibits growth in a rabbit model. Growth Hormone & IGF Research, 24, S14-S15.
- Characterization of an unusual bipolar helicase encoded by bacteriophage T5.. Nucleic Acids Res, 41(8), 4587-4600. View this article in WRRO
- Ligand-Receptor Fusion Dimers, 227-235.
- The structure of Escherichia coli ExoIX—implications for DNA binding and catalysis in flap endonucleases. Nucleic Acids Research. View this article in WRRO
- OR03-1 Development of a platform technology to generate long-acting protein therapeutics. Growth Hormone & IGF Research, 22, S6-S7.
- P02-27 Challenges encountered in the development of an in vivo mouse model system for testing growth hormone antagonist molecules. Growth Hormone & IGF Research, 22, S63-S63.
- Expression of a glycosylphosphatidylinositol-anchored ligand, growth hormone, blocks receptor signalling.. Biosci Rep, 32(6), 653-660. View this article in WRRO
- Biologics, biosimilars and biobetters in protein hormone therapy: Natural synthesis. Biochemist, 34(1), 10-15.
- Neutralizing mutations of carboxylates that bind metal 2 in T5 flap endonuclease result in an enzyme that still requires two metal ions.. J Biol Chem, 286(35), 30878-30887.
- A model for transition of 5′-nuclease domain of DNA polymerase I from inert to active modes. PLoS ONE, 6(1). View this article in WRRO
- Brønsted analysis and rate-limiting steps for the T5 flap endonuclease catalyzed hydrolysis of exonucleolytic substrates.. Biochemistry, 49(37), 8085-8093.
- Immunogenicity, toxicology, pharmacokinetics and pharmacodynamics of growth hormone ligand-receptor fusions.. Clin Sci (Lond), 119(11), 483-491.
- Active site substitutions delineate distinct classes of eubacterial flap endonuclease.. Biochem J, 418(2), 285-292. View this article in WRRO
- Three metal ions participate in the reaction catalyzed by T5 flap endonuclease.. J Biol Chem, 283(42), 28741-28746.
- The Sheffield spin-off experience: Asterion. Biochemist, 30(2), 8-12.
- Investigating the interaction between osteoprotegerin and receptor activator of NF-kappaB or tumor necrosis factor-related apoptosis-inducing ligand: evidence for a pivotal role for osteoprotegerin in regulating two distinct pathways.. J Biol Chem, 282(43), 31601-31609.
- A ligand-receptor fusion of growth hormone forms a dimer and is a potent long-acting agonist.. Nat Med, 13(9), 1108-1113.
- Comparison of the catalytic parameters and reaction specificities of a phage and an archaeal flap endonuclease.. J Mol Biol, 371(1), 34-48.
- Relaxed cleavage specificity of an immunoglobulin A1 protease from Neisseria meningitidis.. Infect Immun, 75(6), 2875-2885.
- Molecular interactions of Escherichia coli ExoIX and identification of its associated 3'-5' exonuclease activity.. Nucleic Acids Res, 35(12), 4094-4102. View this article in WRRO
- Schistosoma mansoni secretes a chemokine binding protein with antiinflammatory activity. J EXP MED, 202(10), 1319-1325. View this article in WRRO
- Immunoglobulin A1 proteases: a structure-function update.. Biochem Soc Trans, 32(Pt 6), 1130-1132.
- Schistosoma mansoni worms induce anergy of T cells via selective up-regulation of programmed death ligand 1 on macrophages. J IMMUNOL, 173(2), 1240-1248.
- Roles of divalent metal ions in flap endonuclease-substrate interactions.. Nat Struct Mol Biol, 11(5), 450-456.
- Dynamic evidence for metal ion catalysis in the reaction mediated by a flap endonuclease.. EMBO J, 22(5), 995-1004.
- A conserved tyrosine residue aids ternary complex formation, but not catalysis, in phage T5 flap endonuclease.. J Mol Biol, 320(5), 1025-1035.
- Interactions of mutant and wild-type flap endonucleases with oligonucleotide substrates suggest an alternative model of DNA binding.. Proc Natl Acad Sci U S A, 99(13), 8542-8547.
- Nontypeable Haemophilus influenzae in carriage and disease: a difference in IgA1 protease activity levels.. JAMA, 287(13), 1699-1705.
- Acres of antibodies: the future of recombinant biomolecule production?. Trends Biotechnol, 19(11), 429-430.
- Continuous affinity-based selection: rapid screening and simultaneous amplification of bacterial surface-display libraries. Biochemical Journal, 357(3), 779-785.
- Continuous affinity-based selection: rapid screening and simultaneous amplification of bacterial surface-display libraries.. Biochem J, 357(Pt 3), 779-785.
- Unusually wide co-factor tolerance in a metalloenzyme; divalent metal ions modulate endo-exonuclease activity in T5 exonuclease.. Nucleic Acids Res, 29(13), 2772-2779.
- Who gives an iota about DNA repair?. Trends Biochem Sci, 26(3), 149-150.
- Differential binding of Mn(II) to bacteriophage T5 5′ nuclease and vital role of metal site II in exonucleolytic cleavage. Biochemical Society Transactions, 29(1), A30-A30.
- Mutagenesis of conserved residues in bacteriophage T5 5′-3′ exonuclease support model of separate mechanisms of endo- and exonucleolytic cleavage. Biochemical Society Transactions, 29(1), A30-A30.
- A new model of DNA binding by bacteriophage T5 exonuclease, a flap endonuclease. Biochemical Society Transactions, 29(1), A30-A30.
- A method for enhancing the transfection efficiency of minipreps obtained from plasmid cDNA libraries.. Anal Biochem, 288(2), 230-232.
- Variation in the steady state kinetic parameters of wild type and mutant T5 5'-3'-exonuclease with pH. Protonation of Lys-83 is critical for DNA binding.. J Biol Chem, 274(25), 17711-17717.
- A helix-loop-helix region of T5 5′-3′ exonuclease contains residues important for substrate and cofactor binding. Biochemical Society Transactions, 27(3), A127-A127.
- A single cleavage assay for T5 5'-->3' exonuclease: determination of the catalytic parameters forwild-type and mutant proteins.. Nucleic Acids Res, 27(3), 730-735.
- Invasive isolates of Neisseria meningitidis possess enhanced immunoglobulin A1 protease activity compared to colonizing strains.. FASEB J, 13(2), 331-337.
- Mutagenesis of conserved lysine residues in bacteriophage T5 5'-3' exonuclease suggests separate mechanisms of endo-and exonucleolytic cleavage.. Proc Natl Acad Sci U S A, 96(1), 38-43.
- Structure-specific DNA cleavage by 5 ' nucleases. TRENDS BIOCHEM SCI, 23(9), 331-336.
- Flexible loops and helical arches.. Nat Struct Biol, 5(8), 668-670.
- Bacteriophage collagen [5] (multiple letters). Science, 279(5358), 1834.
- Bacteriophage collagen. SCIENCE, 279(5358), 1834-1834.
- Prokaryotic 5'-3' exonucleases share a common core structure with gamma-delta resolvase.. Nucleic Acids Res, 25(21), 4224-4229.
- Structure-specific DNA binding by bacteriophage T5 5'-->3' exonuclease.. Nucleic Acids Res, 25(19), 3801-3807.
- Schistosoma mansoni: anomalous immunogenic properties of a 27 kDa larval serine protease associated with protective immunity.. Parasitology, 115 ( Pt 3), 237-247.
- Cloning, heterologous expression and antigenicity of a schistosome cercarial protease. PARASITOLOGY, 114, 447-453.
- Identification and eradication of a denatured DNA isolated during alkaline lysis-based plasmid purification procedures.. Anal Biochem, 241(2), 186-189.
- A helical arch allowing single-stranded DNA to thread through T5 5'-exonuclease. NATURE, 382(6586), 90-93.
- Rare codon usage in Escherichia coli and the expression of potentially toxic genes - Reply. PARASITOLOGY TODAY, 12(3), 124-125.
- Viral polymerase-associated 5'-->3'-exonucleases: expression, purification, and uses.. Methods Enzymol, 275, 227-238.
- AGA/AGG CODON USAGE IN PARASITES - IMPLICATIONS FOR GENE-EXPRESSION IN ESCHERICHIA-COLI. PARASITOL TODAY, 11(9), 345-346.
- The evolution of pathways for aromatic hydrocarbon oxidation in Pseudomonas.. Biodegradation, 5(3-4), 195-217.
- Computer aided identification of a potential 5'-3' exonuclease gene encoded by Escherichia coli.. J Theor Biol, 170(4), 415-421.
- Two identical copies of IS1246, a 1275 base pair sequence related to other bacterial insertion sequences, enclose the xyl genes on TOL plasmid pWW0.. Microbiology, 140 ( Pt 9), 2305-2307.
- Preliminary crystallographic studies on the D15 5' to 3' exonuclease from phage T5.. J Mol Biol, 233(1), 179-182.
- SITE-DIRECTED MUTAGENESIS OF SINGLE-STRANDED AND DOUBLE-STRANDED DNA BY PHOSPHOROTHIOATE APPROACH. METHODS IN ENZYMOLOGY, 217, 189-217.
- IDENTICAL RESOLVASES ARE ENCODED BY PSEUDOMONAS TOL PLASMIDS PWW53 AND PDK1. NUCLEIC ACIDS RESEARCH, 20(20), 5476-5476.
- RAPID HIGH-EFFICIENCY SITE-DIRECTED MUTAGENESIS BY THE PHOSPHOROTHIOATE APPROACH. BIOTECHNIQUES, 13(4), 592-596.
- High Efficiency Oligonucleotide-Directed Mutagenesis of Double-stranded DNA Vectors. Expert Opinion on Therapeutic Patents, 2(7), 1023-1029.
- A SINGLE-STRAND SPECIFIC ENDONUCLEASE ACTIVITY COPURIFIES WITH OVEREXPRESSED T5 D15 EXONUCLEASE. NUCLEIC ACIDS RESEARCH, 19(15), 4127-4132.
- Properties of overexpressed phage T5 D15 exonuclease. Similarities with Escherichia coli DNA polymerase I 5'-3' exonuclease.. J Biol Chem, 265(30), 18311-18317.
- Inhibition of the restriction endonuclease BanII using modified DNA substrates. Determination of phosphate residues critical for the formation of an active enzyme-DNA complex.. J Biol Chem, 265(24), 14389-14394.
- Inhibition of restriction endonuclease hydrolysis by phosphorothioate-containing DNA.. Nucleic Acids Res, 17(22), 9495.
- Phosphorothioate-Based Site-Directed Mutagenesis: Extending the Basic Methodology. Nucleosides and Nucleotides, 7(5-6), 625-628.
- 5'-3' exonucleases in phosphorothioate-based oligonucleotide-directed mutagenesis.. Nucleic Acids Res, 16(3), 791-802.
- Strand specific cleavage of phosphorothioate-containing DNA by reaction with restriction endonucleases in the presence of ethidium bromide.. Nucleic Acids Res, 16(3), 803-814.
- Synthesis and antiviral properties of (E)-5-(2-bromovinyl)-2'-deoxycytidine-related compounds.. J Med Chem, 31(1), 268-271.
- The synthesis and antiviral properties of (E)-5-(2-bromovinyl)-2'-deoxyuridine-related compounds. Tetrahedron, 43(20), 4601-4608.
- High SARS-CoV-2 incidence and asymptomatic fraction during Delta and Omicron BA.1 waves in The Gambia. Nature Communications, 15(1).
- Antibiotics Limit Adaptation of Drug-Resistant Staphylococcus aureus to Hypoxia. Antimicrobial Agents and Chemotherapy.
- Risk factors for SARS-CoV-2 seroprevalence following the first pandemic wave in UK healthcare workers in a large NHS Foundation Trust. Wellcome Open Research, 6, 220-220.
- Risk factors for SARS-CoV-2 seroprevalence following the first pandemic wave in UK healthcare workers in a large NHS Foundation Trust. Wellcome Open Research, 6, 220-220.
- A GH antagonist fusion with GH binding protein is biologically active, shows delayed clearance and inhibits growth in a rabbit model. Endocrine Abstracts.
- Glycosylated linkers to generate long-acting GH tandems. Endocrine Abstracts, 1-1.
- Sunny Chicago and gloomy Oxford: their roles in a citation classic. Biochemical Journal, c1-c1.
Chapters
- Bacteriophage T5 In Calendar R (Ed.), The Bacteriophages (pp. 268-276). Oxford University Press
- Phosphorothioate-based site-directed mutageneis In Creighton TE (Ed.), Protein function (pp. 279-295). Oxford University Press, USA
- Phosphorothioate-based site directed mutagenesis In McPherson MJ (Ed.), Directed mutagenesis (pp. 49-59). Oxford University Press, USA
- Phosphorothioate-based oligonucleotide-directed mutagenesis In Setlow JK (Ed.), Genetic Engineering (pp. 109-122). Plenum Pub Corp
Conference proceedings papers
- Immunogenicity, Toxicology, Pharmacokinetics and Pharmacodynamics of Growth Hormone Ligand-Receptor Fusions.. ENDOCRINE REVIEWS, Vol. 31(3)
- Comh: Isolation, purification and characterisation of a unique H-pylori protein. GASTROENTEROLOGY, Vol. 132(4) (pp A212-A213)
- ComH: Isolation, purification and characterisation of a unique H pylori protein. GUT, Vol. 56 (pp A55-A55)
- ComH: Purification of a unique H pylori protein. GUT, Vol. 54 (pp A86-A86)
- Isolation of a novel TNF regulating protein.. ARTHRITIS AND RHEUMATISM, Vol. 42(9) (pp S201-S201)
- Mechanistic studies of a 5 ' nuclease from T5 bacteriophage. CHEMISTRY OF NUCLEIC ACID COMPONENTS, Vol. 2 (pp 161-167)
- PHOSPHOROTHIOATE BASED MUTAGENESIS OF SINGLE AND DOUBLE-STRANDED DNA VECTORS. SITE-DIRECTED MUTAGENESIS AND PROTEIN ENGINEERING (pp 171-179)
- INTERACTION OF RESTRICTION ENDONUCLEASES WITH PHOSPHOROTHIOATE-CONTAINING DNA. NUCLEOSIDES & NUCLEOTIDES, Vol. 10(1-3) (pp 665-667)
- PHOSPHOROTHIOATE-DNA FOR OLIGONUCLEOTIDE-DIRECTED MUTAGENESIS. BIOLOGICAL CHEMISTRY HOPPE-SEYLER, Vol. 368(9) (pp 1036-1036)
Patents
- Modified growth hormone fusion polypeptides. US7524649 B2 (USA). Appl. 28 Apr 2009.
- MODIFIED GROWTH HORMONE POLYPEPTIDES. WO/2009/047474 Appl. 16 Apr 2009.
- GROWTH HORMONE FUSION PROTEINS. WO/2009/013461 Appl. 29 Jan 2009.
- Growth Factors. Appl. 01 Dec 2006.
- Designer proteases. US6,383,775 (USA). Appl. 01 Jan 1970.
- Growth hormone fusion proteins. 8,470,559 (USA). Appl. 01 Jan 1970.
- Fusion protein comprising growth hormone and growth hormone receptor. 7,446,183 Appl. 01 Jan 1970.
- Cytokine polypeptides and antibodies containing a signal sequence for the attachment of glycosylphosphatidylinositol. US 7,625,998 (USA). Appl. 01 Jan 1970.
Preprints
- Flap endonuclease Substrate Interactions studied using Dynamic Single-Molecule Atomic Force Microscopy, Cold Spring Harbor Laboratory.
- Efficient overexpression and purification of SARS-CoV-2 Nucleocapsid proteins in Escherichia coli, Cold Spring Harbor Laboratory.
- A high content microscopy-based platform for detecting antibodies to the nucleocapsid, spike and membrane proteins of SARS-CoV-2, Cold Spring Harbor Laboratory.
- Risk factors for SARS-CoV-2 seroprevalence following the first pandemic wave in UK healthcare workers in a large NHS Foundation Trust.
- Efficient overexpression and purification of severe acute respiratory syndrome coronavirus 2 nucleocapsid proteins in Escherichia coli. Biochemical Journal, 481(11), 669-682. View this article in WRRO
- Teaching interests
-
I teach graduate-level biochemistry, molecular & structural biology, bioinformatics and biotechnology in relation to biomedical research and drug discovery.
- Professional activities and memberships
-
- Director, Asterion Ltd, 2001 - 2009.
- Trustee, Biochemical Society Pension Scheme, 2008 - 2017
- Honorary Treasurer and Trustee of the Biochemical Society, 2008-2012.
- Director of Portland Press, 2008-2012.
- Editorial board member of The Biochemical Journal, 2007 - 2015.
- Founding Director, DeFENition Ltd.
Granted patents:
- Ross R, Artymiuk P, Sayers J. Growth hormone fusion proteins. 2013, Granted US Patent No. 8,470,559.
(PLEASE NOTE although this has the same title and author as patent no. 8,293,709 which was already included, it is a separate patent granted in 2013). - Ross R, Artymiuk P, Sayers J. Growth hormone fusion proteins. 2012, Granted US Patent No. 8,293,709.
- Artymiuk, P, Ross R, Sayers J. Modified growth hormone polypeptides 2012, Granted US Patent No. 8,273,552.
- Ross R, Artymiuk P, Sayers J. Fusion protein comprising growth hormone and growth hormone receptor. 2012 Granted US Patent No. 8,173,782.
- Ross R, Sayers J, Artymiuk P. Modified growth hormone fusion polypeptides, 2009, Granted US Patent No. 7524649.
- Ross R, Sayers J, Artymiuk P. Cytokine polypeptides and antibodies containing a signal sequence for the attachment of glycosylphosphatidylinositol, 2009, Granted US Patent No. 7625998.
- Ross R, Artymiuk P, Sayers J. Fusion protein comprising growth hormone and growth hormone receptor. 2008 Granted US Patent No. 7,446,183.
- Duff, G.W., Sayers, J.R., Vitovski, S. Designer proteases. 2002 Granted US Patent No.6,383,775.