Dr Richmond Muimo

PhD

Clinical Medicine, School of Medicine and Population Health

Lecturer in Cell Biology

r.muimo@sheffield.ac.uk
+44 114 215 9515

+44 114 271 1863 (fax)

The Medical School

Full contact details

Dr Richmond Muimo
Clinical Medicine, School of Medicine and Population Health
The Medical School
Beech Hill Road
Sheffield
S10 2RX

Profile

For enquiries please contact - ClinMed-Operational@sheffield.ac.uk

After completing my PhD at the University of Leeds in 1993, I took up a post as a PostDoctoral Researcher. In 1993 I became a Research Fellow at Dundee University where I went on to become a Lecturer in 2001.

I joined The University of Sheffield in 2003 where I continue to work as a Lecturer.

Current PhD Opportunities

Immune responses and leukocyte function - Role of tyrosine and histidine phosphorylation

Research interests

I am focused on understanding the mechanisms that regulate ion transport in secretory epithelia, particularly in relation to cystic fibrosis. I have identified a number of CFTR-associated proteins that are involved in regulating ion channel function.

Over the past few years my interests have expanded into understanding the role of CFTR-associated proteins in neutrophil function and inflammation.

Current Projects

Application of stable analogues of phosphohistidine to the study of cell signaling pathways.
Nucleoside diphosphate kinase (NDPK) regulates CFTR function.
Roles of NDPK in neutrophilic inflammation.

Publications

Show: Featured publications All publications

Featured publications

Journal articles

Mambwe B, Neo K, Javanmard Khameneh H, Leong KWK, Colantuoni M, Vacca M, Muimo R & Mortellaro A (2019) Tyrosine dephosphorylation of ASC modulates the activation of the NLRP3 and AIM2 inflammasomes. Frontiers in Immunology, 10. View this article in WRRO
Borthwick LA, Neal A, Hobson L, Gerke V, Robson L & Muimo R (2008) The annexin 2-S100A10 complex and its association with TRPV6 is regulated by cAMP/PKA/CnA in airway and gut epithelia.. Cell Calcium, 44(2), 147-157.
Borthwick LA, Riemen C, Goddard C, Colledge WH, Mehta A, Gerke V & Muimo R (2008) Defective formation of PKA/CnA-dependent annexin 2-S100A10/CFTR complex in DeltaF508 cystic fibrosis cells.. Cell Signal, 20(6), 1073-1083.
Borthwick LA, McGaw J, Conner G, Taylor CJ, Gerke V, Mehta A, Robson L & Muimo R (2007) The formation of the cAMP/protein kinase A-dependent annexin 2-S100A10 complex with cystic fibrosis conductance regulator protein (CFTR) regulates CFTR channel function.. Mol Biol Cell, 18(9), 3388-3397.
Muimo R, Crawford RM & Mehta A (2006) Nucleoside diphosphate kinase A as a controller of AMP-kinase in airway epithelia.. J Bioenerg Biomembr, 38(3-4), 181-187.
Muimo R, Hornickova Z, Riemen CE, Gerke V, Matthews H & Mehta A (2000) Histidine phosphorylation of annexin I in airway epithelia.. J Biol Chem, 275(47), 36632-36636.
Makwana M, dos Santos Souza C, Pickup BT, Thompson, MJ, Lomada SK, Feng Y, Wieland T, Jackson RFW & Muimo R () Chemical tools for study of phosphohistidine: generation of selective Τ‐phosphohistidine and Π‐phosphohistidine antibodies. ChemBioChem. View this article in WRRO

All publications

Journal articles

Makwana MV, Williamson MP, Jackson RFW & Muimo R (2022) Quantitation of phosphohistidine in proteins in a mammalian cell line by 31P NMR. PLoS ONE, 17(9).
Mambwe B, Neo K, Javanmard Khameneh H, Leong KWK, Colantuoni M, Vacca M, Muimo R & Mortellaro A (2019) Tyrosine dephosphorylation of ASC modulates the activation of the NLRP3 and AIM2 inflammasomes. Frontiers in Immunology, 10. View this article in WRRO
Makwana MV, Muimo R & Jackson RFW (2019) Correction: Advances in development of new tools for the study of phosphohistidine. Laboratory Investigation, 99(3), 438-438.
Muimo R, Alothaid HMM & Mehta A (2018) NM23 proteins: innocent bystanders or local energy boosters for CFTR?. Laboratory Investigation, 98(3), 272-282. View this article in WRRO
Makwana MV, Muimo R & Jackson RFW (2018) Advances in development of new tools for the study of phosphohistidine. Laboratory Investigation, 98(3), 291-303. View this article in WRRO
Attwood PV & Muimo R (2018) The actions of NME1/NDPK-A and NME2/NDPK-B as protein kinases. Laboratory Investigation, 98(3), 283-290. View this article in WRRO
Muimo R (2017) Classical activation of macrophages and vardenafil.. Clin Sci (Lond), 131(11), 1141-1145. View this article in WRRO
Borthwick LA, Kerbiriou M, Taylor CJ, Cozza G, Lascu I, Postel EH, Cassidy D, Trouvé P, Mehta A, Robson L & Muimo R (2016) Role of Interaction and Nucleoside Diphosphate Kinase B in Regulation of the Cystic Fibrosis Transmembrane Conductance Regulator Function by cAMP-Dependent Protein Kinase A. PLoS ONE, 11(3), e0149097-e0149097. View this article in WRRO
Lilley M, Mambwe B, Thompson MJ, Jackson RFW & Muimo R (2015) 4-Phosphopyrazol-2-yl alanine: a non-hydrolysable analogue of phosphohistidine. Chem. Commun., 51(34), 7305-7308. View this article in WRRO
Lilley M, Mambwe B, Jackson RFW & Muimo R (2014) 4-Phosphothiophen-2-yl alanine: a new 5-membered analogue of phosphotyrosine.. Chem Commun (Camb), 50(66), 9343-9345. View this article in WRRO
King JD, Lee J, Riemen CE, Neumann D, Xiong S, Foskett JK, Mehta A, Muimo R & Hallows KR (2012) Role of binding and nucleoside diphosphate kinase A in the regulation of the cystic fibrosis transmembrane conductance regulator by AMP-activated protein kinase.. J Biol Chem, 287(40), 33389-33400. View this article in WRRO
Little R, Muimo R, Robson L, Harris K & Grabowski PS (2011) The transient receptor potential ion channel TRPV6 is expressed at low levels in osteoblasts and has little role in osteoblast calcium uptake. PLoS ONE, 6(11). View this article in WRRO
England AK, Muimo R & Robson L (2011) THE ROLE OF TYROSINE PHOSPHORYLATION IN REGULATING CFTR FUNCTION IN EPITHELIAL CELLS. PEDIATR PULM, 250-250.
Jr KJD, Lee J, Rieman CE, Mehta A, Muimo R & Hallows KR (2010) Role of Binding and Nucleoside Diphosphate Kinase A (NDPK-A) in the Regulation of CFTR by AMP-Activated Protein Kinase (AMPK). FASEB JOURNAL, 24.
Muimo R, Nagaraj V, Palekar S & Robson L (2009) EFFECT OF RUTHENIUM RED AND cAMP ON Ca2+ HANDLING IN BREAST CANCER CELLS. J PHYSIOL SCI, 59, 249-249.
Harris KL, Muimo R & Robson L (2009) CFTR PLAYS A ROLE IN REGULATING CALCIUM PERMEABLE ION CHANNELS IN AIRWAY EPITHELIA. J PHYSIOL SCI, 59, 299-299.
Muimo R (2009) A ROLE FOR NUCLEOSIDE DIPHOSPHATE KINASE-B (NDPK-B) IN REGULATION OF CFTR BY HETEROTRIMERIC G-PROTEINS IN AIRWAY EPITHELIA. PEDIATR PULM, 224-224.
Borthwick LA, Neal A, Hobson L, Gerke V, Robson L & Muimo R (2008) The annexin 2-S100A10 complex and its association with TRPV6 is regulated by cAMP/PKA/CnA in airway and gut epithelia.. Cell Calcium, 44(2), 147-157.
Borthwick LA, Riemen C, Goddard C, Colledge WH, Mehta A, Gerke V & Muimo R (2008) Defective formation of PKA/CnA-dependent annexin 2-S100A10/CFTR complex in DeltaF508 cystic fibrosis cells.. Cell Signal, 20(6), 1073-1083.
Borthwick LA, McGaw J, Conner G, Taylor CJ, Gerke V, Mehta A, Robson L & Muimo R (2007) The formation of the cAMP/protein kinase A-dependent annexin 2-S100A10 complex with cystic fibrosis conductance regulator protein (CFTR) regulates CFTR channel function.. Mol Biol Cell, 18(9), 3388-3397.
Muimo R, Crawford RM & Mehta A (2006) Nucleoside diphosphate kinase A as a controller of AMP-kinase in airway epithelia.. J Bioenerg Biomembr, 38(3-4), 181-187.
Mwimbi XKMS, Muimo R, Green MW & Mehta A (2003) Making human nasal cilia beat in the cold: a real time assay for cell signalling.. Cell Signal, 15(4), 395-402.
Mwimbi XKMS, Muimo R, Treharne KJ, Sijumbila G, Green M & Mehta A (2002) 4alpha-Phorbol negates the inhibitory effects of phorbol-12-myristate-13-acetate on human cilia and alters the phosphorylation of PKC.. FEBS Lett, 530(1-3), 31-36.
Ghioni C, Treharne KJ, Mehta A, Olver RE, Land SC & Muimo R (2002) Effect of AMPK activation on fatty acid metabolism in epithelia. Biochemical Society Transactions, 30(5), A121-A121.
Treharne KJ, Mehta A & Muimo R (2002) Improved oxygenation promotes nucleoside diphosphate kinase activity in a human bronchial epithelial cell line (HBE). J PHYSIOL-LONDON, 539, 18P-18P.
Best OG, Treharne KJ, Goddard C, Colledge W, Muimo R & Mehta A (2002) Novel regulation of the nucleotide generator NDPK by the AMP-activated kinase. J PHYSIOL-LONDON, 539, 24P-24P.
Treharne KJ, Riemen CE, Marshall LJ, Muimo R & Mehta A (2001) Nucleoside diphosphate kinase--a component of the [Na(+)]- and [Cl(-)]-sensitive phosphorylation cascade in human and murine airway epithelium.. Pflugers Arch, 443 Suppl 1, S97-102.
Muimo R, Hornickova Z, Riemen CE, Gerke V, Matthews H & Mehta A (2000) Histidine phosphorylation of annexin I in airway epithelia.. J Biol Chem, 275(47), 36632-36636.
Best OG, Mehta A & Muimo R (2000) A 62 kDa cAMP-binding protein as a putative regulator of nucleoside diphosphate kinase activity. J PHYSIOL-LONDON, 527, 24P-25P.
Mwimbi X, Muimo R & Mehta A (2000) Protein kinase C mitigates against the flow-dependent decline in human nasal ciliary beat frequency in vitro. J PHYSIOL-LONDON, 527, 30P-31P.
Mukhopadhyay S, Muimo R, Campbell FM, Gordon MJ, Monaghan AS & Dutta-Roy AK (2000) Preferential distribution of long chain polyunsaturated fatty acids in phospatidyl ethanolamine fraction of guinea pig alveolar apical membranes.. Prostaglandins Leukot Essent Fatty Acids, 62(6), 341-348.
Mwimbi XK, Muimo R, Green M & Mehta A (2000) Protein kinase C regulates the flow rate-dependent decline in human nasal ciliary beat frequency in vitro.. J Aerosol Med, 13(3), 273-279.
Monaghan AS, Baines DL, Muimo R & Olver RE (1999) Inwardly rectifying K+ currents in fetal alveolar type II cells: regulation by protein kinase A and protein phosphatases.. Pflugers Arch, 438(3), 371-377.
Marshall LJ, Muimo R, Riemen CE & Mehta A (1999) Na+ and K+ regulate the phosphorylation state of nucleoside diphosphate kinase in human airway epithelium.. Am J Physiol, 276(1), C109-C119.
Marshall LJ, Muimo R & Mehta A (1998) [Na+] regulates nucleoside diphosphate kinase in human respiratory epithelium in vitro when ATP (but not GTP) is the kinase substrate. J PHYSIOL-LONDON, 509P, 81P-82P.
Muimo R, Hornickova Z & Mehta A (1998) The chloride-dependent phosphorylated protein in apical membranes from ovine airway is annexin I. J PHYSIOL-LONDON, 509P, 82P-82P.
Riemen CE, Muimo R, Ratcliff RA, Colledge WH, Evans MJ & Mehta A (1998) Defective nucleoside triphosphate synthesis in plasma membranes derived from the tracheal epithelium of CFTR null (-/-) mice. J PHYSIOL-LONDON, 509P, 81P-81P.
Marshall LJ, Muimo R & Mehta A (1998) Na+ and K+ differentially regulate nucleoside diphosphate kinase in human respiratory epithelium in vitro. J PHYSIOL-LONDON, 507P, 3P-4P.
Muimo R, Banner SJ, Marshall LJ & Mehta A (1998) Nucleoside diphosphate kinase and Cl(-)-sensitive protein phosphorylation in apical membranes from ovine airway epithelium.. Am J Respir Cell Mol Biol, 18(2), 270-278.
Banner SJ, Marshall LJ, Muimo R & Mehta A (1996) Intermolecular transfer of guanine nucleotide gamma phosphate to apical membrane proteins in ovine tracheal epithelium in vitro. J PHYSIOL-LONDON, 493P, P72-P72.
Meyer DJ, Muimo R, Thomas M, Coates D & Isaac RE (1996) Purification and characterization of prostaglandin-H E-isomerase, a sigma-class glutathione S-transferase, from Ascaridia galli.. Biochem J, 313 ( Pt 1), 223-227.
Muimo R & Isaac RE (1993) Properties of an arylalkylamine N-acetyltransferase from the nematode, Ascaridia galli.. Comp Biochem Physiol B, 106(4), 969-976.
Muimo R & Isaac RE (1993) Metabolism of 5-HT in the nematode Caenorhabditis elegans: identification of a 5-HT N-acetyltransferase that is developmentally regulated.. Biochem Soc Trans, 21 ( Pt 3)(3), 295S.
MUIMO R & ISAAC RE (1993) N-ACETYLATION OF 5-HT IS A MAJOR METABOLIC PATHWAY IN NEMATODES AND IS DEVELOPMENTALLY REGULATED IN C-ELEGANS. J CELL BIOCHEM, 117-117.
Isaac RE, Eaves L, Muimo R & Lamango N (1991) N-acetylation of biogenic amines in Ascaridia galli.. Parasitology, 102 Pt 3, 445-450.
Isaac RE, Muimo R & MacGregor AN (1990) N-acetylation of serotonin, octopamine and dopamine by adult Brugia pahangi.. Mol Biochem Parasitol, 43(2), 193-198.
Makwana M, dos Santos Souza C, Pickup BT, Thompson, MJ, Lomada SK, Feng Y, Wieland T, Jackson RFW & Muimo R () Chemical tools for study of phosphohistidine: generation of selective Τ‐phosphohistidine and Π‐phosphohistidine antibodies. ChemBioChem. View this article in WRRO
Makwana MV, van Meurs S, Hounslow AM, Williamson MP, Jackson, RFW & Muimo R () 31P NMR Spectroscopy Demonstrates Large Amounts of Phosphohistidine in Mammalian Cells.
Makwana M, Dos Santos Souza C, Pickup BT, Thompson MJ, Lomada SK, Feng Y, Wieland T, F. W. Jackson R & MUIMO R () Chemical Tools for Study of Phosphohistidine: Generation of Selective τ-Phosphohistidine and π-Phosphohistidine Antibodies.

Conference proceedings papers

Mambwe B, Muimo R & Mortellaro A (2019) Analysing the Role of Tyrosine Phosphorylation in Inflammasome Complex Formation and Function. CARDIOVASCULAR DRUGS AND THERAPY, Vol. 33(2) (pp 270-270)
Jr KJD, Lee J, Rieman CE, Mehta A, Muimo R & Hallows KR (2012) Role of Binding and Nucleoside Diphosphate Kinase A (NDPK-A) in the Regulation of CFTR by AMP-Activated Protein Kinase (AMPK). FASEB JOURNAL, Vol. 26
England AK, Muimo R & Robson L (2011) A potential role of tyrosine phosphorylation in the function of the cystic fibrosis transmembrane conductance regulator (CFTR). FASEB JOURNAL, Vol. 25
Muimo R (2009) Regulation of CFTR function by annexin A2-S100A10 complex in health and disease. GENERAL PHYSIOLOGY AND BIOPHYSICS, Vol. 28 (pp F14-F19)
Mwimbi X, Muimo R & Mehta A (2001) Presentation of ciliary beat frequency results to our peers. CILIA AND MUCUS: FROM DEVELOPMENT TO RESPIRATORY DEFENSE (pp 91-97)
Muimo R, Banner SJ, Marshall L & Mehta A (1998) The nucleoside diphosphate kinase and chloride-sensitive GTP-dependent phosphorylation in apical membrane from ciliated airway cells. CILIA, MUCUS, AND MUCOCILIARY INTERACTIONS (pp 39-42)

Teaching interests: My teaching interests lie in aspects of membrane biology and invertebrate model systems which I teach on the MBChB and MSc in Molecular Medicine, respectively.

Professional activities and memberships

Biochemical Society
European Calcium Society
American Society For Cell Biology