Professor Mark Dickman
School of Chemical, Materials and Biological Engineering
Professor in Bioanalytical Science and Engineering
Personal Chair
Chair of Research Committtee
m.dickman@sheffield.ac.uk
+44 114 222 7541
+44 114 222 7541
Room D54, Sir Robert Hadfield Building
Full contact details
Professor Mark Dickman
School of Chemical, Materials and Biological Engineering
Room D54
Sir Robert Hadfield Building
Mappin Street
Sheffield
S1 3JD
School of Chemical, Materials and Biological Engineering
Room D54
Sir Robert Hadfield Building
Mappin Street
Sheffield
S1 3JD
- Profile
-
I obtained a First Class Honours Degree in Biochemistry/Chemistry at the University of Sheffield. I continued at the Krebs Institute, where I obtained my PhD. Following my PhD I joined a biotechnology company, Transgenomic LTD where I worked as a research scientist developing analytical techniques including DNA/RNA Chromatography. I joined the Dept of Chemical and Biological Engineering in 2003.
- Research interests
-
Nucleic acid therapeutics including oligonucleotide therapeutics and mRNA vaccines/therapeutics are emerging as an important new class of drugs to treat a wide range of important diseases. My research focuses on the development and application analytical techniques to study biological molecules. In particular, bioseparations in conjunction with biological mass spectrometry are used to characterise and analyse nucleic acid therapeutics.
Key research interests:
- Bioseparations (HPLC)
- Biological Mass Spectrometry
- Nucleic Acids
- Oligonucleotide therapeutics
- mRNA vaccines/therapeutics
- RNAi biocontrols
- Analytics.
Industry Partners include:
- ThermoFisher
- AstraZeneca
- Syngenta
- RedShift Bio
- Publications
-
Journal articles
- An Experimental and Modeling Approach to Study Tangential Flow Filtration Performance for mRNA Drug Substance Purification. Biotechnology Journal, 19(11).
- Quality by design for mRNA platform purification based on continuous oligo-dT chromatography. Molecular Therapy - Nucleic Acids, 102333-102333.
- Anion exchange HPLC monitoring of mRNA in vitro transcription reactions to support mRNA manufacturing process development. Frontiers in Molecular Biosciences, 11. View this article in WRRO
- Optimisation of denaturing ion pair reversed phase HPLC for the purification of ssDNA in SELEX.. Journal of Chromatography A, 464699-464699.
- Clostridioides difficile canonical L,D-transpeptidases catalyze a novel type of peptidoglycan cross-links and are not required for beta-lactam resistance. Journal of Biological Chemistry, 300(1). View this article in WRRO
- Unusual 1-3 peptidoglycan cross-links in Acetobacteraceae are made by L,D-transpeptidases with a catalytic domain distantly related to YkuD domains. Journal of Biological Chemistry, 300(1), 105494-105494.
- The master energy homeostasis regulator PGC-1α exhibits an mRNA nuclear export function. Nature Communications, 14. View this article in WRRO
- Optimization of orthogonal separations for the analysis of oligonucleotides using 2D-LC. Journal of Chromatography B, 1227, 123812-123812.
- STN7 is not essential for developmental acclimation of Arabidopsis to light intensity. The Plant Journal.
- An automated, low volume, and high-throughput analytical platform for aggregate quantitation from cell culture media. Journal of Chromatography A, 1691.
- Efficient synthesis of DNA duplexes containing reduced acetaldehyde interstrand cross-links. Journal of the American Chemical Society, 145(2), 953-959.
- Absolute quantification of cellular levels of photosynthesis-related proteins in Synechocystis sp. PCC 6803. Photosynthesis Research.
- FtsH4 protease controls the biogenesis of PSII complex by dual regulation of high light-inducible proteins. Plant Communications.
- Strategies for the production of dsRNA biocontrols as alternatives to chemical pesticides. Frontiers in Bioengineering and Biotechnology, 10.
- Chemically modified dsRNA induces RNAi effects in insects in vitro and in vivo: a potential new tool for improving RNA-based plant protection. Journal of Biological Chemistry, 298(9).
- Characterization and sequence mapping of large RNA and mRNA therapeutics using mass spectrometry. Analytical Chemistry.
- Molecular basis of specificity and deamidation of eIF4A by Burkholderia lethal factor 1. Communications Biology, 5(1). View this article in WRRO
- High-throughput multiplex analysis of mAb aggregates and charge variants by automated two-dimensional size exclusion-cation exchange chromatography coupled to mass spectrometry. Journal of Chromatography A, 1670.
- Changes in supramolecular organization of cyanobacterial thylakoid membrane complexes in response to far-red light photoacclimation. Science Advances, 8(6).
- Comparative proteomics of thylakoids from Arabidopsis grown in laboratory and field conditions. Plant Direct, 5(10). View this article in WRRO
- The effects of plant cysteine proteinases on the nematode cuticle. Parasites & Vectors, 14(1).
- Pneumolysin Is Responsible for Differential Gene Expression and Modifications in the Epigenetic Landscape of Primary Monocyte Derived Macrophages. Frontiers in Immunology, 12, 573266.
- How the O2-dependent Mg-protoporphyrin monomethyl ester cyclase forms the fifth ring of chlorophylls. Nature Plants, 7(3), 365-375.
- Structures of Rhodopseudomonas palustris RC-LH1 complexes with open or closed quinone channels. Science Advances, 7(3), eabe2631-eabe2631.
- Developmental acclimation of the thylakoid proteome to light intensity in Arabidopsis. The Plant Journal.
- Xanthophyll carotenoids stabilise the association of cyanobacterial chlorophyll synthase with the LHC-like protein HliD. Biochemical Journal. View this article in WRRO
- Proteorhodopsin overproduction enhances the long-term viability of Escherichia coli. Applied and Environmental Microbiology, 86(1). View this article in WRRO
- Depletion of the FtsH1/3 Proteolytic Complex Suppresses the Nutrient Stress Response in the Cyanobacterium Synechocystis sp strain PCC 6803. The Plant Cell, 31(12), 2912-2928.
- Membrane organization of photosystem I complexes in the most abundant phototroph on Earth. Nature Plants, 5(8), 879-889. View this article in WRRO
- The ChlD subunit links the motor and porphyrin binding subunits of magnesium chelatase. Biochemical Journal. View this article in WRRO
- Comparison of data acquisition methods for the identification and quantification of histone post‐translational modifications on a Q Exactive HF hybrid quadrupole Orbitrap mass spectrometer.. Rapid Communications in Mass Spectrometry, 33(10), 897-906. View this article in WRRO
- Proteins that physically interact with the phosphatase Cdc14 in Candida albicans have diverse roles in the cell cycle. Scientific Reports, 9. View this article in WRRO
- High resolution fingerprinting of single and double-stranded RNA using ion-pair reverse phase chromatography. Journal of Chromatography B, 1104, 212-219. View this article in WRRO
- Proteomic and cellular localisation studies suggest non-tight junction cytoplasmic and nuclear roles for occludin in astrocytes. European Journal of Neuroscience, 47(12), 1444-1456. View this article in WRRO
- UCHL3 Regulates Topoisomerase-Induced Chromosomal Break Repair by Controlling TDP1 Proteostasis. Cell Reports, 23(11), 3352-3365. View this article in WRRO
- Quantification of dsRNA using stable isotope labeling dilution liquid chromatography/mass spectrometry. Rapid Communications in Mass Spectrometry, 32(7), 590-596. View this article in WRRO
- Identification of protein W, the elusive sixth subunit of the Rhodopseudomonas palustris reaction center-light harvesting 1 core complex. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1859(2), 119-128. View this article in WRRO
- Bacteriophage DNA glucosylation impairs target DNA binding by type I and II but not by type V CRISPR–Cas effector complexes. Nucleic Acids Research, 46(2), 873-885. View this article in WRRO
- Quantitative Proteomic Analysis in Candida albicans
Using SILAC-Based Mass Spectrometry. PROTEOMICS, 18(5-6), 1700278-1700278. View this article in WRRO
- Accurate Quantification of Nucleic Acids Using Hypochromicity Measurements in Conjunction with UV Spectrophotometry. Analytical Chemistry, 89(24), 13567-13574. View this article in WRRO
- PufQ regulates porphyrin flux at the haem/bacteriochlorophyll branchpoint of tetrapyrrole biosynthesis via interactions with ferrochelatase.. Molecular Microbiology. View this article in WRRO
- Purification and characterisation of dsRNA using ion pair reverse phase chromatography and mass spectrometry. Journal of Chromatography A, 1484, 14-25. View this article in WRRO
- RNASwift: a rapid, versatile RNA extraction method free from phenol and chloroform. Analytical Biochemistry, 512, 36-46. View this article in WRRO
- Biosynthesis of Chlorophyll a in a Purple Bacterial Phototroph and Assembly into a Plant Chlorophyll–Protein Complex. ACS Synthetic Biology, 5(9), 948-954. View this article in WRRO
- The therapeutic potential of epigenetic manipulation during infectious diseases. Pharmacology & Therapeutics. View this article in WRRO
- Analysis of histone post translational modifications in primary monocyte derived macrophages using reverse phase × reverse phase chromatography in conjunction with porous graphitic carbon stationary phase. Journal of Chromatography A, 1453, 43-53. View this article in WRRO
- Two Unrelated 8-Vinyl Reductases Ensure Production of Mature Chlorophylls in Acaryochloris marina. Journal of Bacteriology, 198(9), 1393-1400. View this article in WRRO
- Nucleic acid separations using superficially porous silica particles. Journal of Chromatography A, 1440, 135-144. View this article in WRRO
- Synthesis of Chlorophyll-Binding Proteins in a Fully Segregated Δycf54 Strain of the Cyanobacterium Synechocystis PCC 6803. Frontiers in Plant Science, 7. View this article in WRRO
- Oxidative Glial Cell Damage Associated with White Matter Lesions in the Aging Human Brain. Brain Pathology, 25(5), 565-574. View this article in WRRO
- Mass spectrometry analysis of histone post translational modifications. Drug Discovery Today: Disease Models, 12, 41-48.
- 227 Luzp4 defines a new mRNA export pathway in cancer cells. European Urology Supplements, 14(2), e227-e227b.
- Arginine methylation and citrullination of splicing factor proline- and glutamine-rich (SFPQ/PSF) regulates its association with mRNA. RNA, 21(3), 347-359. View this article in WRRO
- Luzp4 defines a new mRNA export pathway in cancer cells. Nucleic Acids Research, 43(4), 2353-2366. View this article in WRRO
- Analysis of crRNA Using Liquid Chromatography Electrospray Ionization Mass Spectrometry (LC ESI MS), 133-145.
- Programmable RNA Shredding by the Type III-A CRISPR-Cas System of Streptococcus thermophilus. Molecular Cell, 56(4), 506-517. View this article in WRRO
- Aberrant Assembly Complexes of the Reaction Center Light-harvesting 1 PufX (RC-LH1-PufX) Core Complex ofRhodobacter sphaeroidesImaged by Atomic Force Microscopy. Journal of Biological Chemistry, 289(43), 29927-29936. View this article in WRRO
- Integration of energy and electron transfer processes in the photosynthetic membrane of Rhodobacter sphaeroides.. Biochim Biophys Acta, 1837(10), 1769-1780. View this article in WRRO
- Archaeal MBF1 binds to 30S and 70S ribosomes via its helix–turn–helix domain. Biochemical Journal, 462(2), 373-384.
- Integration of energy and electron transfer processes in the photosynthetic membrane of Rhodobacter sphaeroides. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1837, e118-e118.
- Sequestration of multiple RNA recognition motif-containing proteins by C9orf72 repeat expansions.. Brain, 137(Pt 7), 2040-2051. View this article in WRRO
- GGGGCC Repeat Expansion of C9ORF72 Is Toxic Via Sequestration Of RNA Binding Proteins And Consequent Disruption Of RNA Splicing (1II-2.005). Neurology, 82(10_supplement).
- GGGGCC Repeat Expansion of C9ORF72 Is Toxic Via Sequestration Of RNA Binding Proteins And Consequent Disruption Of RNA Splicing (S56.004). Neurology, 82(10_supplement).
- A cyanobacterial chlorophyll synthase-HliD complex associates with the Ycf39 protein and the YidC/Alb3 insertase.. Plant Cell, 26(3), 1267-1279.
- Cofractionation of HMGB proteins with histone dimers.. Anal Biochem, 447, 98-106.
- Three-dimensional structure of the Rhodobacter sphaeroides RC-LH1-PufX complex: dimerization and quinone channels promoted by PufX.. Biochemistry, 52(43), 7575-7585.
- Affinity filtration coupled with capillary-based affinity purification for the isolation of protein complexes.. Anal Biochem, 439(1), 47-49.
- In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus. EMBO Journal, 32(3), 385-394. View this article in WRRO
- Identification of an 8-vinyl reductase involved in bacteriochlorophyll biosynthesis in Rhodobacter sphaeroides and evidence for the existence of a third distinct class of the enzyme.. Biochem J, 450(2), 397-405.
- Extensive histone post-translational modification in honey bees.. Insect Biochem Mol Biol, 43(2), 125-137.
- Efficient purification of chromatin architectural proteins: Histones, HMGB proteins and FKBP3 (FKBP25) immunophilin. RSC Advances, 2(28), 10598-10604.
- Conserved chloroplast open-reading frame ycf54 is required for activity of the magnesium protoporphyrin monomethylester oxidative cyclase in Synechocystis PCC 6803.. J Biol Chem, 287(33), 27823-27833.
- Quantitative proteomic analysis of intracytoplasmic membrane development in Rhodobacter sphaeroides.. Mol Microbiol, 84(6), 1062-1078.
- Rapid resonance Raman microspectroscopy to probe carbon dioxide fixation by single cells in microbial communities.. ISME J, 6(4), 875-885.
- A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A.. Science, 334(6057), 821-824. View this article in WRRO
- Correction for Wiedenheft et al., RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions. Proceedings of the National Academy of Sciences, 108(36), 15010-15010.
- Characterization and quantification of RNA post-transcriptional modifications using stable isotope labeling of RNA in conjunction with mass spectrometry analysis.. Anal Chem, 83(12), 4894-4901.
- RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions.. Proc Natl Acad Sci U S A, 108(25), 10092-10097.
- Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Nature Structural and Molecular Biology, 18(5), 529-536.
- Shotgun proteome analysis utilising mixed mode (reversed phase-anion exchange chromatography) in conjunction with reversed phase liquid chromatography mass spectrometry analysis.. Proteomics, 10(16), 2950-2960.
- Arginine methylation of REF/ALY promotes efficient handover of mRNA to TAP/NXF1.. Nucleic Acids Res, 38(10), 3351-3361. View this article in WRRO
- Analysis of arginine and lysine methylation utilizing peptide separations at neutral pH and electron transfer dissociation mass spectrometry.. J Am Soc Mass Spectrom, 21(1), 88-96.
- UIF, a New mRNA export adaptor that works together with REF/ALY, requires FACT for recruitment to mRNA.. Curr Biol, 19(22), 1918-1924.
- Post-translational modifications of the linker histone variants and their association with cell mechanisms. FEBS J, 276(14), 4109-4121.
- Studying the mechanism of RNA separations using RNA chromatography and its application in the analysis of ribosomal RNA and RNA:RNA interactions.. J Chromatogr A, 1216(9), 1377-1382.
- Characterization of post-translational modifications of the linker histories H1 and H5 from chicken erythrocytes using mass spectrometry. J PROTEOME RES, 7(10), 4326-4335.
- Small CRISPR RNAs guide antiviral defense in prokaryotes. SCIENCE, 321(5891), 960-964. View this article in WRRO
- Identification and characterization of sulfolobus solfataricus P2 proteome using multidimensional liquid phase protein separations.. J Proteome Res, 7(6), 2253-2261.
- Multidimensional liquid phase protein separations in conjunction with stable isotope labelling for quantitative proteomics.. Proteomics, 7(21), 3826-3834.
- Regulation of p73-mediated apoptosis by c-Jun N-terminal kinase. BIOCHEM J, 405, 617-623.
- Postcolumn nucleic acid intercalation for the fluorescent detection of nucleic acids using ion pair reverse phase high-performance liquid chromatography.. Anal Biochem, 360(2), 282-287.
- Enrichment and analysis of RNA centered on ion pair reverse phase methodology.. RNA, 12(4), 691-696.
- Micro-scale open-tube capillary separations of functional proteins. ANAL BIOCHEM, 350(1), 128-137.
- Effects of sequence and structure in the separation of nucleic acids using ion pair reverse phase liquid chromatography.. J Chromatogr A, 1076(1-2), 83-89.
- Rapid analysis of protein-nucleic acid complexes using MALDI TOF mass spectrometry and ion pair reverse phase liquid chromatography.. J Biochem Biophys Methods, 58(1), 39-48.
- Identification of nuclear protein complexes using open tube capillary chromatography. AIChE Annual Meeting, Conference Proceedings, 1917.
- The effect of tautomeric constant on the specificity of nucleotide incorporation during DNA replication: support for the rare tautomer hypothesis of substitution mutagenesis.. J Mol Biol, 326(5), 1389-1401.
- The RuvABC resolvasome.. Eur J Biochem, 269(22), 5492-5501.
- Use of fluorescent DNA-intercalating dyes in the analysis of DNA via ion-pair reversed-phase denaturing high-performance liquid chromatography.. Anal Biochem, 309(2), 248-252.
- Zebularine: a novel DNA methylation inhibitor that forms a covalent complex with DNA methyltransferases.. J Mol Biol, 321(4), 591-599.
- Holliday junction binding and processing by the RuvA protein of Mycoplasma pneumoniae.. Eur J Biochem, 269(5), 1525-1533.
- High-throughput analysis of nucleic acid modification reactions using ion-pair reverse-phase high-performance liquid chromatography.. Anal Biochem, 301(2), 290-297.
- RNA footprinting analysis using ion pair reverse phase liquid chromatography.. RNA, 8(2), 247-251.
- Rapid, high precision analysis of DNA and RNA footprinting reactions utilising the WAVE (R) Nucleic Acid Fragment Analysis System.. AM J HUM GENET, 69(4), 468-468.
- Rapid analysis of biochemical modifications in DNA fragments - Visualisation of phosphorylation and cytosine deamination in single 15 minute analyses.. AM J HUM GENET, 69(4), 462-462.
- Analysis of nucleic acid modification enzyme reactions using IP RP HPLC. J MED GENET, 38, S71-S71.
- Enhancing the catalytic repertoire of nucleic acids: a systematic study of linker length and rigidity.. Nucleic Acids Res, 29(7), 1565-1573.
- Isolation of single-stranded DNA using denaturing DNA chromatography.. Anal Biochem, 284(1), 164-167.
- Small Crispr Rnas Guide Antiviral Defense in Prokaryotes. Cancer Epidemiology Biomarkers and Prevention, 2(6), 531-535.
- Glycoprofile Comparison of the SARS-CoV-2 Spike Proteins Expressed in CHO and HEK Cell Lines. Molecular Biotechnology.
- Optimizing the production of dsRNA biocontrols in microbial systems using multiple transcriptional terminators. Biotechnology and Bioengineering.
- Production of Trimeric SARS‐CoV‐2 Spike Protein by CHO Cells for Serological COVID‐19 Testing. Biotechnology and Bioengineering.
- Pneumolysin is responsible for differential gene expression and modifications in the epigenetic landscape of primary monocyte derived macrophages.
- Correction: Analysis of long dsRNA produced in vitro and in vivo using atomic force microscopy in conjunction with ion-pair reverse-phase HPLC. The Analyst, 144(22), 6773-6773.
- Analysis of long dsRNA produced in vitro and in vivo using atomic force microscopy in conjunction with ion-pair reverse-phase HPLC. Analyst. View this article in WRRO
Chapters
- Chromatin: The Biophysical Approach to Elucidating Structure and Function In Leroy NH & Fournier NT (Ed.), Cell cycle control (pp. 411-428). New York: Nova Biomedical.
Conference proceedings papers
- Defining the molecular targets for plant cysteine proteinases on parasitic nematode extracellular matrix. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, Vol. 96(2) (pp A9-A10)
- Characterization of Streptococcus thermophilus Csm complex mediated CRISPR interference in vitro. FEBS JOURNAL, Vol. 281 (pp 678-678)
- 411: Epigenetic mechanisms of cadmium urothelial carcinogenesis. European Journal of Cancer, Vol. 50 (pp S98-S98)
- Oxidative stress and DNA damage in cerebral white matter lesions of the ageing human brain. NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY, Vol. 40 (pp 33-34)
- Oxidative stress and DNA damage in cerebral white matter lesions of the ageing human brain. NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY, Vol. 39 (pp 36-37)
- Analysis of the RNA component of human telomerase and its associated activity.. AMERICAN JOURNAL OF HUMAN GENETICS, Vol. 71(4) (pp 333-333)
Preprints
- The evolution and developmental dynamics of histone-based chromatin regulation in Annelida, Cold Spring Harbor Laboratory.
- Improving tangential flow filtration for mRNA medicines purification, Authorea, Inc..
- GRASPS: a simple-to-operate translatome technology reveals omics-hidden disease-associated pathways in TDP-43-related amyotrophic lateral sclerosis, Cold Spring Harbor Laboratory.
- Optimising the production of dsRNA biocontrols in microbial systems using multiple transcriptional terminators, Cold Spring Harbor Laboratory.
- Unusual 1-3 peptidoglycan cross-links inAcetobacteraceaeare made by L,D-transpeptidases with a catalytic domain distantly related to YkuD domains, Cold Spring Harbor Laboratory.
- Characterisation and sequence mapping of large RNA and mRNA therapeutics using mass spectrometry, Cold Spring Harbor Laboratory.
- Chemically modified dsRNA induces RNAi effects in insects in vitro and in vivo: A potential new tool for improving RNA-based plant protection, Cold Spring Harbor Laboratory.
- The master energy homeostasis regulator PGC-1α couples transcriptional co-activation and mRNA nuclear export, Cold Spring Harbor Laboratory.
- Production of Trimeric SARS-CoV-2 Spike Protein by CHO Cells for Serological COVID-19 Testing, Cold Spring Harbor Laboratory.
- Production of Trimeric SARS-CoV-2 Spike Protein by CHO Cells for Serological COVID-19 Testing, Authorea, Inc..
- Purification of recombinant SARS-CoV-2 spike, its receptor binding domain, and CR3022 mAb for serological assay.
- A proteome-wide screen reveals widespread roles for the phosphatase Cdc14 in theCandida albicanscell cycle.
- An Experimental and Modeling Approach to Study Tangential Flow Filtration Performance for mRNA Drug Substance Purification. Biotechnology Journal, 19(11).
- Teaching activities
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CPE 240 Engineering From Living Systems 2
- Professional activities and memberships
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- Member Royal Society of Chemistry,
- Analytical Bioscience Committee Member (RSC)
- Member of the American Society for Mass Spectrometry
- Industrial partners
We work closely with a number of industrial partners including Syngenta, GlaxoSmithKline, and ThermoFisher.