Professor David James
School of Chemical, Materials and Biological Engineering
Professor of Bioprocess Engineering
Research Centre Director Advanced Biomanufacturing Centre
d.c.james@sheffield.ac.uk
+44 114 222 7505
+44 114 222 7505
Room D62, Sir Robert Hadfield Building
Full contact details
Professor David James
School of Chemical, Materials and Biological Engineering
Room D62
Sir Robert Hadfield Building
Mappin Street
Sheffield
S1 3JD
School of Chemical, Materials and Biological Engineering
Room D62
Sir Robert Hadfield Building
Mappin Street
Sheffield
S1 3JD
- Profile
-
I graduated in 1989 from King´s College London with a Ph.D. in Biochemistry, followed by postdoctoral research in Wye College (University of London), then the University of Kent; the latter a joint project with the Advanced Centre for Biochemical Engineering at University College London.
Following appointment to Lecturer, then Senior Lecturer at Kent, I moved to the University of Queensland in 2001 as an Associate Professor of Bioengineering, then Professorial Research Fellow in the Australian Institute of Bioengineering and Nanotechnology.
I was appointed to a Chair in Bioprocess Engineering at Sheffield in 2006 with a Royal Society Wolfson Research Merit Award, Research and Training in Biopharmaceutical Bioprocessing for UK Bioindustry.
- Research interests
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- Production of high-value therapeutic recombinant protein biopharmaceuticals
- Systems Biotechnology for Rational Bioprocess Engineering
- Cell Factory and Gene Vector Engineering
- Process Analytical Technology
- Publications
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Books
- Engineering Protein Folding and Secretion in Eukaryotic Cell Factories.
- Engineering Mammalian Cells for Recombinant Monoclonal Antibody Production. Springer Netherlands.
Journal articles
- Molecular design of controllable recombinant adeno‐associated virus (AAV) expression systems for enhanced vector production. Biotechnology Journal, 19(6), e2300685.
- Directed evolution of biomass intensive CHO cells by adaptation to sub-physiological temperature. Metabolic Engineering, 81, 53-69.
- Protein-specific signal peptides for mammalian vector engineering. ACS Synthetic Biology, 12(8), 2339-2352. View this article in WRRO
- CHO synthetic promoters improve expression and product quality of biotherapeutic proteins. Biotechnology Progress.
- An automated, low volume, and high-throughput analytical platform for aggregate quantitation from cell culture media. Journal of Chromatography A, 1691, 463809-463809.
- Increased recombinant adeno‐associated virus production by HEK293 cells using small molecule chemical additives. Biotechnology Journal.
- Risk factors for SARS-CoV-2 seroprevalence following the first pandemic wave in UK healthcare workers in a large NHS Foundation Trust. Wellcome Open Research, 6, 220.
- Engineering of the CMV promoter for controlled expression of recombinant genes in HEK293 cells. Biotechnology Journal. View this article in WRRO
- Bioinformatic design of dendritic cell-specific synthetic promoters. ACS Synthetic Biology, 11(4), 1613-1626. View this article in WRRO
- High-throughput multiplex analysis of mAb aggregates and charge variants by automated two-dimensional size exclusion-cation exchange chromatography coupled to mass spectrometry. Journal of Chromatography A, 462944-462944.
- Risk factors for SARS-CoV-2 seroprevalence following the first pandemic wave in UK healthcare workers in a large NHS Foundation Trust. Wellcome Open Research, 6. View this article in WRRO
- Control of Multigene Expression Stoichiometry in Mammalian Cells Using Synthetic Promoters.. ACS Synth Biol.
- Design of synthetic promoters for controlled expression of therapeutic genes in retinal pigment epithelial cells. Biotechnology and Bioengineering. View this article in WRRO
- Cell function profiling to assess clone stability. Biotechnology and Bioengineering, 117(7), 2295-2299.
- A platform for context-specific genetic engineering of recombinant protein production by CHO cells. Journal of Biotechnology, 312, 11-22.
- Resveratrol addition to CHO cell culture media: The effect on cell growth, monoclonal antibody synthesis and its chemical modification. Biotechnology Progress. View this article in WRRO
- Screening naturally occurring phenolic antioxidants for their suitability as additives to CHO cell culture media used to produce monoclonal antibodies. Antioxidants, 8(6). View this article in WRRO
- Comparison of data acquisition methods for the identification and quantification of histone post‐translational modifications on a Q Exactive HF hybrid quadrupole Orbitrap mass spectrometer.. Rapid Communications in Mass Spectrometry, 33(10), 897-906. View this article in WRRO
- CHO Genome Mining for Synthetic Promoter Design. Journal of Biotechnology. View this article in WRRO
- Whole synthetic pathway engineering of recombinant protein production. Biotechnology and Bioengineering, 116(2), 375-387. View this article in WRRO
- Control of amino acid transport into Chinese hamster ovary cells. Biotechnology and Bioengineering, 115(12), 2908-2929. View this article in WRRO
- Highly sensitive detection of mutations in CHO cell recombinant DNA using multi-parallel single molecule real-time DNA sequencing. Biotechnology and Bioengineering, 115(6), 1485-1498. View this article in WRRO
- Metabolic phenotyping of CHO cells varying in cellular biomass accumulation and maintenance during fed-batch culture. Biotechnol Bioeng, 115(3), 645-660. View this article in WRRO
- In silico design of context-responsive mammalian promoters with user-defined functionality. Nucleic Acids Research, 45(18), 10906-10919. View this article in WRRO
- High-throughput quantitation of Fc-containing recombinant proteins in cell culture supernatant by fluorescence polarization spectroscopy. Analytical Biochemistry, 534, 49-55.
- Transcriptome-based identification of the optimal reference CHO genes for normalisation of qPCR data. Biotechnology Journal. View this article in WRRO
- Constructing Strong Cell Type-Specific Promoters Through Informed Design, 131-145.
- Precision control of recombinant gene transcription for CHO cell synthetic biology. Biotechnology Advances, 34(5), 492-503.
- Importance of Interaction between Integrin and Actin Cytoskeleton in Suspension Adaptation of CHO cells. Applied Biochemistry and Biotechnology, 178(7), 1286-1302.
- Integrated cell and process engineering for improved transient production of a “difficult-to-express“ fusion protein by CHO cells. Biotechnology and Bioengineering, 112(12), 2527-2542.
- NF-κB, CRE and YY1 elements are key functional regulators of CMV promoter-driven transient gene expression in CHO cells. Biotechnology Journal, 10(7), 1019-1028.
- Synthetic promoters for CHO cell engineering.. Biotechnol Bioeng, 111(8), 1638-1647.
- A mechanistic dissection of polyethylenimine mediated transfection of CHO cells: to enhance the efficiency of recombinant DNA utilization.. Biotechnol Prog, 30(5), 1161-1170.
- Predicting the expression of recombinant monoclonal antibodies in Chinese hamster ovary cells based on sequence features of the CDR3 domain.. Biotechnol Prog, 30(1), 188-197.
- Model-directed engineering of "difficult-to-express" monoclonal antibody production by Chinese hamster ovary cells.. Biotechnol Bioeng, 111(2), 372-385.
- Block decoys: transcription-factor decoys designed for in vitro gene regulation studies.. Anal Biochem, 443(2), 205-210.
- CHO cell line specific prediction and control of recombinant monoclonal antibody N-glycosylation.. Biotechnol Bioeng, 110(11), 2970-2983.
- Functional heterogeneity and heritability in CHO cell populations.. Biotechnol Bioeng, 110(1), 260-274.
- Expression of a glycosylphosphatidylinositol-anchored ligand, growth hormone, blocks receptor signalling.. Biosci Rep, 32(6), 653-660. View this article in WRRO
- Cell line specific control of polyethylenimine-mediated transient transfection optimized with "Design of experiments" methodology.. Biotechnol Prog, 28(1), 179-187.
- Impact of gene vector design on the control of recombinant monoclonal antibody production by Chinese hamster ovary cells.. Biotechnol Prog, 27(6), 1689-1699.
- A mechanistic understanding of production instability in CHO cell lines expressing recombinant monoclonal antibodies.. Biotechnol Bioeng, 108(10), 2434-2446.
- An empirical modeling platform to evaluate the relative control discrete CHO cell synthetic processes exert over recombinant monoclonal antibody production process titer.. Biotechnol Bioeng, 108(9), 2193-2204.
- Cell line-specific control of recombinant monoclonal antibody production by CHO cells.. Biotechnol Bioeng, 106(6), 938-951.
- Systems biotechnology of mammalian cell factories.. Brief Funct Genomic Proteomic, 7(2), 95-110.
- Dynamic analysis of GS-NS0 cells producing a recombinant monoclonal antibody during fed-batch culture. BIOTECHNOL BIOENG, 97(2), 410-424.
- Transient Gene Expression Levels from Multigene Expression Vectors. Biotechnology Progress, 23(2), 435-443.
- Functional proteomic analysis of GS-NS0 murine myeloma cell lines with varying recombinant monoclonal antibody production rate. Biotechnology and Bioengineering, 94(5), 830-841.
- Monitoring changes in nisin susceptibility of Listeria monocytogenes Scott A as an indicator of growth phase using FACS. Journal of Microbiological Methods, 66(1), 43-55.
- On the statistical analysis of the GS-NS0 cell proteome: Imputation, clustering and variability testing. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 1764(7), 1179-1187.
- Control of Culture Environment for Improved Polyethylenimine-Mediated Transient Production of Recombinant Monoclonal Antibodies by CHO Cells. Biotechnology Progress, 22(3), 753-762.
- Proteomic analysis of enriched microsomal fractions from GS-NS0 murine myeloma cells with varying secreted recombinant monoclonal antibody productivities. PROTEOMICS, 5(18), 4689-4704.
- A platform for high-throughput molecular characterization of recombinant monoclonal antibodies. Journal of Chromatography B, 826(1-2), 177-187.
- Control of Recombinant Monoclonal Antibody Effector Functions by Fc N-Glycan Remodeling in Vitro. Biotechnology Progress, 21(6), 1644-1652.
- Engineering mammalian cell factories for improved recombinant monoclonal antibody production: lessons from nature?. Biotechnology and Bioengineering, 91(2), 180-189.
- Transient production of recombinant proteins by Chinese hamster ovary cells using polyethyleneimine/DNA complexes in combination with microtubule disrupting anti-mitotic agents. Biotechnology and Bioengineering, 88(6), 707-721.
- Comparative proteomic analysis of GS-NS0 murine myeloma cell lines with varying recombinant monoclonal antibody production rate. Biotechnology and Bioengineering, 88(4), 474-488.
- Global changes in gene expression observed at the transition from growth to stationary phase in
Listeria monocytogenes
ScottA batch culture. PROTEOMICS, 4(1), 123-135.
- Fatty acids as haptens: exploring the limits of antigenicity. Molecular Immunology, 40(6), 381-389.
- Evaluation of individual protein errors in silver-stained two-dimensional gels. Biochemical and Biophysical Research Communications, 306(4), 1050-1055.
- Engineering mRNA Translation Initiation to Enhance Transient Gene Expression in Chinese Hamster Ovary Cells. Biotechnology Progress, 19(1), 121-129.
- Rapid monitoring of recombinant protein products: a comparison of current technologies. Trends in Biotechnology, 20(4), 149-156.
- Protein modification during anti-viral heat-treatment bioprocessing of factor VIII concentrates, factor IX concentrates, and model proteins in the presence of sucrose. Biotechnology and Bioengineering, 77(1), 37-48.
- Metabolic control of recombinant monoclonal antibodyN-glycosylation in GS-NS0 cells. Biotechnology and Bioengineering, 75(2), 239-251.
- Protein Modifications during Antiviral Heat Bioprocessing and Subsequent Storage. Biotechnology Progress, 17(5), 974-978.
- Metabolic control of recombinant proteinN-glycan processing in NS0 and CHO cells. Biotechnology and Bioengineering, 73(3), 188-202.
- Evaluation of protein modification during anti-viral heat bioprocessing by electrospray ionization mass spectrometry. Rapid Communications in Mass Spectrometry, 15(5), 351-356.
- Mechanisms of protein modification during model anti-viral heat-treatment bioprocessing of β-lactoglobulin variant A in the presence of sucrose. Biotechnology and Applied Biochemistry, 32(2), 109-109.
- Metabolic control of recombinant monoclonal antibody N-glycosylation in GS-NS0 cells. GLYCOBIOLOGY, 10(10), 1108-1108.
- Protein modification during antiviral heat bioprocessing. Biotechnology and Bioengineering, 67(2), 177-188.
- Metabolic Engineering of Recombinant Protein Sialylation. Biochemical Society Transactions, 28(5), A259-A259.
- Constraints on the transport and glycosylation of recombinant IFN-? in Chinese hamster ovary and insect cells. Biotechnology and Bioengineering, 63(5), 559-572.
- Characterisation of glycated albumin using electrospray ionisation mass spectrometry. Clinical Chemistry and Laboratory Medicine, 37(SPEC. SUPPL.), S520-S520.
- Efficient Human IFN-gamma Expression in the Mammary Gland of Transgenic Mice. Journal of Interferon & Cytokine Research, 19(2), 137-144.
- Monitoring recombinant human interferon-gamma N-glycosylation during perfused fluidized-bed and stirred-tank batch culture of CHO cells. Biotechnology and Bioengineering, 60(5), 596-607.
- Review: The Glycosylation Heterogeneity of Recombinant Human IFN-γ. Journal of Interferon & Cytokine Research, 18(5), 287-295.
- Real-time monitoring of recombinant protein concentration in animal cell cultures using an optical biosensor. Genetic Engineer and Biotechnologist, 17(2-3), 69-74.
- . Cytotechnology, 23(1/3), 103-111.
- Getting the glycosylation right: Implications for the biotechnology industry. Nature Biotechnology, 14(8), 975-981.
- Posttranslational processing of recombinant human interferon-γ in animal expression systems. Protein Science, 5(2), 331-340.
- Analysis of recombinat glycoproteins by mass spectrometry. Cytotechnology, 22(1-3), 17-24.
- N-glycans of recombinant human interferon-? change during batch culture of chinese hamster ovary cells. Biotechnology and Bioengineering, 48(6), 639-648.
- N-Glycosylation of Recombinant Human Interferon-γ Produced in Different Animal Expression Systems. Nature Biotechnology, 13(6), 592-596.
- High-Resolution Separation of Recombinant Human Interferon-γ Glycoforms by Micellar Electrokinetic Capillary Chromatography. Analytical Biochemistry, 222(2), 315-322.
- PURIFICATION AND CHARACTERIZATION OF A NITRILASE FROM BRASSICA-NAPUS. PHYSIOLOGIA PLANTARUM, 89(4), 811-816.
- DEVELOPMENT AND CHARACTERISTICS OF MYROSINASE IN BRASSICA-NAPUS DURING EARLY SEEDLING GROWTH. PHYSIOLOGIA PLANTARUM, 82(2), 163-170.
- Biosynthesis of (R)-2-hydroxybut-3-enylglucosinolate (progoitrin) from [3,4-3H]but-3-enylglucosinolate in Brassica napus. Journal of the Chemical Society, Perkin Transactions 1(7), 1909-1909.
- BIOSYNTHESIS OF R-2 HYDROXYBUT-3-ENYLGLUCOSINOLATE PROGOITRIN FROM 3 4 TRITIATED BUT-3-ENYLGLUCOSINOLATE IN BRASSICA-NAPUS. Journal of the Chemical Society Perkin Transactions I(7), 1909-1914.
- Biosynthesis of 2-hydroxy-3-butenylglucosinolate and 3-butenylglucosinolate in Brassica napus. Phytochemistry, 29(8), 2509-2512.
- Glycoprofile Comparison of the SARS-CoV-2 Spike Proteins Expressed in CHO and HEK Cell Lines. Molecular Biotechnology.
- Risk factors for SARS-CoV-2 seroprevalence following the first pandemic wave in UK healthcare workers in a large NHS Foundation Trust. Wellcome Open Research, 6, 220-220.
- ACE2-Independent Interaction of SARS-CoV-2 Spike Protein with Human Epithelial Cells Is Inhibited by Unfractionated Heparin. Cells, 10(6), 1419-1419.
- Production of Trimeric SARS‐CoV‐2 Spike Protein by CHO Cells for Serological COVID‐19 Testing. Biotechnology and Bioengineering.
- The use of catechins in Chinese hamster ovary cell media for the improvement of monoclonal antibody yields and a reduction of acidic species. Biotechnology Progress.
- On the Effect of Transient Expression of Mutated eIF2α and eIF4E Eukaryotic Translation Initiation Factors on Reporter Gene Expression in Mammalian Cells Upon Cold-Shock. Molecular Biotechnology, 34(2), 141-150.
- On the Optimal Ratio of Heavy to Light Chain Genes for Efficient Recombinant Antibody Production by CHO Cells. Biotechnology Progress, 21(1), 122-133.
- Analysis of Glycoprotein Heterogeneity by Capillary Electrophoresis and Mass Spectrometry. Molecular Biotechnology, 14(3), 241-249.
- Monitoring Recombinant Glycoprotein Heterogeneity, 277-288.
Chapters
- Engineering protein folding and secretion in eukaryotic cell factories, Comprehensive Biotechnology (pp. 405-412).
- Protein Therapeutics Wiley-VCH Verlag GmbH & Co. KGaA
- Engineering Protein Folding and Secretion in Eukaryotic Cell Factories, Comprehensive Biotechnology (pp. 419-426). Elsevier
- 1.29 Engineering Protein Folding and Secretion in Eukaryotic Cell Factories, Comprehensive Biotechnology (pp. 405-412).
- Analysis of Glycoprotein Heterogeneity by Capillary Electrophoresis and Mass Spectrometry, Springer Protocols Handbooks (pp. 1335-1346). Humana Press
- Glycosylation of Recombinant Proteins, Encyclopedia of Bioprocess Technology
- High Resolution Glycoform Analysis of Recombinant Human Interferon-Gamma During Batch Cultures of Chinese Hamster Ovary Cells, Animal Cell Technology: Basic & Applied Aspects (pp. 315-321). Springer Netherlands
- Expression Systems for Recombinant Biopharmaceutical Production by Mammalian Cells in Culture, Methods and Principles in Medicinal Chemistry (pp. 423-467). Wiley-VCH Verlag GmbH & Co. KGaA
- Characterization of Therapeutic Proteins by Membrane and In-Gel Tryptic Digestion, Therapeutic Proteins (pp. 375-380). Humana Press
- Sample Preparation Procedures for High-Resolution Nuclear Magnetic Resonance Studies of Aqueous and Stabilized Solutions of Therapeutic Peptides, Therapeutic Proteins (pp. 461-470). Humana Press
- The impact of cooking on protein antigenicity, Food Allergy and Intolerance (pp. 150-161). Royal Society of Chemistry
Conference proceedings papers
- Small molecule chemical additives for enhanced recombinant AAV production in HEK293 cells. HUMAN GENE THERAPY, Vol. 33(23-24) (pp A105-A105)
- Proteomic Profiling Of Two-Dimensional Gel Electrophoresis Protein Expression Data. AIP Conference Proceedings
- How can thermal processing modify the antigenicity of proteins?. Allergy, Vol. 56(s67) (pp 56-60)
- Control of therapeutic monoclonal antibody glycosylation. ANIMAL CELL TECHNOLOGY: PRODUCTS FROM CELLS, CELLS AS PRODUCTS (pp 255-257)
- Resolution glycoform analysis of recombinant human interferon-gamma during batch cultures of Chinese hamster ovary cells. ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS, VOL 8 (pp 315-321)
- Control of Interferon-Gamma Glycosylation by the Addition of Defined Lipid Supplements to Batch Cultures of Recombinant Chinese Hamster Ovary Cells (pp 339-345)
- Epitope Determination for Antibodies Raised Against Recombinant Human Interferon-Gamma (pp 277-282)
- A COMPARISON OF 2 ELECTROPHORETIC METHODS FOR ANALYSING RECOMBINANT PROTEIN GLYCOFORMS. 1995 ICHEME RESEARCH EVENT - FIRST EUROPEAN CONFERENCE FOR YOUNG RESEARCHERS IN CHEMICAL ENGINEERING, VOLS 1 AND 2 (pp 896-898)
- The Functional Competence of Animal Cells: Anal Ysis of the Secretory Pathway (pp 71-74)
- Optimisation of PEI-Mediated Transient Expression in Chinese Hamster Ovary Cells (pp 655-657)
- Metabolic Engineering of Recombinant Plasminogen Sialylation in Animal Cells by Tetracycline-Regulated Expression of Murine CMP-NeuAc Synthetase and Hamster CMP-NeuAc Transporter Genes (pp 263-265)
Patents
- In silico design of mammalian promoters with user-defined functionality. WO 2018/193072 A1 Appl. 25 Oct 2018.
- Synthetic promoters for CHO cells, methods of producing synthetic promoters using transcription factor binding site modules. WO2015079053-A2; WO2015079053-A3 Appl. 08 Oct 2015.
- Regulating recombinant gene expression in vitro comprises providing a host cell encoding recombinant genes for expression, and contacting the cell with an exonuclease resistant block decoy. WO2014202640-A1 Appl. 24 Dec 2014.
- Predicting relative production titer of a panel of clonal producer cells in fed batch culture by simultaneously incubating each producer cell with a chemical cell stressor and determining the production titer response of each cell. EP2902493-A1; WO2015113704-A1 Appl. 01 Jan 1970.
- Method for determining e.g. cell identity of mammalian producer of user, involves comparing query cell-specific growth response fingerprint with reference cell-specific growth response fingerprints corresponding to cells. WO2014207166-A1 Appl. 01 Jan 1970.
Theses / Dissertations
- Screening and development of natural compounds as tools to improve IgG production and quality in recombinant CHO cell culture.
Preprints
- Large-Scale Comparative Transcriptomic Analysis of CHO Cell Functional Adaptation to Recombinant Monoclonal Antibody Production, Cold Spring Harbor Laboratory.
- Protein-specific signal peptides for mammalian vector engineering, Cold Spring Harbor Laboratory.
- Bayesian Optimization for Synthetic Gene Design, arXiv.
- Risk factors for SARS-CoV-2 seroprevalence following the first pandemic wave in UK healthcare workers in a large NHS Foundation Trust.
- Production of Trimeric SARS-CoV-2 Spike Protein by CHO Cells for Serological COVID-19 Testing, Cold Spring Harbor Laboratory.
- Production of Trimeric SARS-CoV-2 Spike Protein by CHO Cells for Serological COVID-19 Testing, Authorea, Inc..
- Purification of recombinant SARS-CoV-2 spike, its receptor binding domain, and CR3022 mAb for serological assay.
- ACE2-independent interaction of SARS-CoV-2 spike protein to human epithelial cells can be inhibited by unfractionated heparin.
- Increased recombinant AAV production by HEK293 cells using small molecule chemical additives, Authorea, Inc..
- Engineering Protein Folding and Secretion in Eukaryotic Cell Factories.
- Teaching activities
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CPE412 – Molecular Biotechnology