Dr Daniel Stevens

BSc, MSc, PhD

School of Medicine and Population Health

Research Technician

daniel.stevens@sheffield.ac.uk
+44 114 215 9579

Room LU108, L Floor, Royal Hallamshire Hospital

Full contact details

Dr Daniel Stevens
School of Medicine and Population Health
Room LU108, L Floor
Royal Hallamshire Hospital
Glossop Road
Sheffield
S10 2JF

Profile

I received my PhD in Cell and Molecular Biology from Newcastle University in 2020, following which I worked in the same laboratory as a postdoctoral researcher in the group of Professor Heath Murray. During this time, I worked to further our understanding of the key process of bacterial DNA replication initiation in the model organism Bacillus subtilis and the pathogen Staphylococcus aureus.

In 2024 I joined the group of Dr Thomas Darton within the Clinical Infection Research Group (CIRG) to provide microbiological experience for the ongoing work to develop models to investigate host-pathogen interactions during staphylococcus aureus carriage and infection.

Qualifications

BSc in Biological Sciences (Genetics)

Msc in Human and Molecular Genetics

PhD in Cell and Molecular Biology

Research interests: My interests are in the bacterial pathogen Staphylococcus aureus. My current work includes characterising clinically relevant strains of S. aureus, involvement in a Staph human challenge model and supporting clinical trials aimed at improving our understanding of the human immune response to staph infection.

Publications

Journal articles

Winterhalter C, Pelliciari S, Stevens D, Fenyk S, Marchand E, Cronin NB, Soultanas P, Costa TRD, Ilangovan A & Murray H (2023) The DNA replication initiation protein DnaD recognises a specific strand of the Bacillus subtilis chromosome origin. Nucleic Acids Research, 51(9), 4322-4340.
Winterhalter C, Stevens D, Fenyk S, Pelliciari S, Marchand E, Soultanas P, Ilangovan A & Murray H (2023) SirA inhibits the essential DnaA:DnaD interaction to block helicase recruitment duringBacillus subtilissporulation. Nucleic Acids Research, 51(9), 4302-4321.
Richardson TT, Stevens D, Pelliciari S, Harran O, Sperlea T & Murray H (2019) Identification of a basal system for unwinding a bacterial chromosome origin. The EMBO Journal, 38(15).
Martin E, Williams HEL, Pitoulias M, Stevens D, Winterhalter C, Craggs TD, Murray H, Searle MS & Soultanas P (2019) DNA replication initiation in Bacillus subtilis: structural and functional characterization of the essential DnaA–DnaD interaction. Nucleic Acids Research, 47(4), 2101-2112.
Pelliciari S, Bodet-Lefèvre S, Fenyk S, Stevens D, Winterhalter C, Schramm FD, Pintar S, Burnham DR, Merces G, Richardson TT , Tashiro Y et al () The bacterial replication origin BUS promotes nucleobase capture. Nature Communications, 14(1).

Preprints

Gaimster H, Stevens D, Hubbard J, Mickiewicz K, Murray H & Winterhalter C (2024) Conflicts between the DNA replication and repair machineries promote cell death in Gram-positive bacteria, Cold Spring Harbor Laboratory.