Dr Janet Chamberlain

BSc PhD SFHEA

Clinical Medicine, School of Medicine and Population Health

Senior University Teacher

J.Chamberlain@sheffield.ac.uk
+44 114 215 9566

+44 114 271 1863 (Fax)

The Medical School

Full contact details

Dr Janet Chamberlain
Clinical Medicine, School of Medicine and Population Health
The Medical School
Beech Hill Road
Sheffield
S10 2RX

Profile

For enquiries please contact - ClinMed-Operational@sheffield.ac.uk

After completing an MRC-funded PhD studentship at the University of Manchester, I spent a year working as an immunohistochemist at Zeneca Pharmaceuticals, Alderley Park. I joined the University of Sheffield in 1995, and spent 3 years as a Postdoctoral Research Associate in the Department of Cardiovascular Research (with Professor David Crossman as PI).

Following this, I wrote my own funding applications, although remained as a Postdoctoral Research Associate, then an Experimental Officer; holding several Project Grants as Principal Investigator, working closely with Professors Sheila Francis and Julian Gunn. In 2016, I became a University Teacher and now concentrate mainly on teaching, although still have an interest in research.

Current PhD Opportunities

Research interests

My research career has focused on the understanding and prevention of restenosis and atherosclerosis, using several different experimental models, including research to develop a miniature stenting device for use in small calibre vessels.

My current areas of research relate to the mechanisms by which IL-1 is released from endothelial and smooth muscle cells, the role of IL-1 in the development of atherosclerosis, and the use of IL-1 antagonists to inhibit disease progression.

In addition, I am currently involved in investigating the role of carbon monoxide as a possible therapeutic for restenosis and myocardial infarction.

Current Projects

Release of IL-1 from endothelial and smooth muscle cells (with Professor Francis).
The use of carbon-monoxide releasing molecules in Restenosis/atherosclerosis (BHF, with Francis/Gunn/Mann).
The use of carbon-monoxide releasing molecules in MI (BHF with Gunn/Iqbal/Francis/Mann).
Cholesterol crystal formation in atherosclerosis (with Francis).

Publications

Show: Featured publications All publications

Featured publications

Journal articles

Alfaidi MA, Chamberlain J, Rothman A, Crossman D, Villa‐Uriol MC, Hadoke P, Wu J, Schenkel T, Evans PC & Francis SE (2018) Dietary Docosahexaenoic Acid Reduces Oscillatory Wall Shear Stress, Atherosclerosis, and Hypertension, Most Likely Mediated via an IL‐1–Mediated Mechanism. Journal of the American Heart Association, 7(13). View this article in WRRO
Renshall L, Arnold N, West L, Braithwaite A, Pickworth J, Walker R, Alfaidi M, Chamberlain J, Casbolt H, Thompson AAR , Holt C et al (2018) Selective improvement of pulmonary arterial hypertension with a dual ET A /ET B receptors antagonist in the apolipoprotein E −/− model of PAH and atherosclerosis. Pulmonary Circulation, 8(1), 1-11. View this article in WRRO
Iqbal J, Chamberlain J, Francis SE & Gunn J (2016) Role of Animal Models in Coronary Stenting. Annals of Biomedical Engineering, 44(2), 453-465.
Alfaidi M, Wilson H, Daigneault M, Burnett A, Ridger V, Chamberlain J & Francis SE (2015) Neutrophil Elastase promotes Interleukin-1β secretion from Human Coronary Endothelium. Journal of Biological Chemistry, 290, 24067-24078. View this article in WRRO
Dénes Á, Pradillo JM, Drake C, Sharp A, Warn P, Murray KN, Rohit B, Dockrell DH, Chamberlain J, Casbolt H , Francis S et al (2014) Streptococcus pneumoniae worsens cerebral ischemia via interleukin 1 and platelet glycoprotein Ibα.. Ann Neurol, 75(5), 670-683. View this article in WRRO
Hameed AG, Arnold ND, Chamberlain J, Pickworth JA, Paiva C, Dawson S, Cross S, Long L, Zhao L, Morrell NW , Crossman DC et al (2012) Inhibition of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) reverses experimental pulmonary hypertension.. J Exp Med, 209(11), 1919-1935. View this article in WRRO
Denes A, Drake C, Stordy J, Chamberlain J, McColl BW, Gram H, Crossman D, Francis S, Allan SM & Rothwell NJ (2012) Interleukin-1 Mediates Neuroinflammatory Changes Associated With Diet-Induced Atherosclerosis. JOURNAL OF THE AMERICAN HEART ASSOCIATION, 1(3). View this article in WRRO
Ellam T, Wilkie M, Chamberlain J, Crossman D, Eastell R, Francis S & Chico TJA (2011) Dietary phosphate modulates atherogenesis and insulin resistance in apolipoprotein E knockout mice--brief report.. Arterioscler Thromb Vasc Biol, 31(9), 1988-1990.
Watt V, Chamberlain J, Steiner T, Francis S & Crossman D (2011) TRAIL attenuates the development of atherosclerosis in apolipoprotein E deficient mice.. Atherosclerosis, 215(2), 348-354.
Lawrie A, Hameed AG, Chamberlain J, Arnold N, Kennerley A, Hopkinson K, Pickworth J, Kiely DG, Crossman DC & Francis SE (2011) Paigen diet-fed apolipoprotein E knockout mice develop severe pulmonary hypertension in an interleukin-1-dependent manner.. Am J Pathol, 179(4), 1693-1705. View this article in WRRO
Chamberlain J, Wheatcroft M, Arnold N, Lupton H, Crossman DC, Gunn J & Francis S (2010) A novel mouse model of in situ stenting.. Cardiovasc Res, 85(1), 38-44. View this article in WRRO
Evans DJW, Jackman LE, Chamberlain J, Crosdale DJ, Judge HM, Jetha K, Norman KE, Francis SE & Storey RF (2009) Platelet P2Y(12) receptor influences the vessel wall response to arterial injury and thrombosis.. Circulation, 119(1), 116-122.
Chamberlain J, Francis S, Brookes Z, Shaw G, Graham D, Alp NJ, Dower S & Crossman DC (2009) Interleukin-1 regulates multiple atherogenic mechanisms in response to fat feeding.. PLoS One, 4(4), e5073. View this article in WRRO
Chamberlain J, Evans D, King A, Dewberry R, Dower S, Crossman D & Francis S (2006) Interleukin-1beta and signaling of interleukin-1 in vascular wall and circulating cells modulates the extent of neointima formation in mice.. Am J Pathol, 168(4), 1396-1403.

All publications

Journal articles

Bazaz R, Marriott HM, Wright C, Chamberlain J, West LE, Gelsthorpe C, Heath PR, Maleki-Dizaji A, Francis SE & Dockrell DH (2023) Transient increase in atherosclerotic plaque macrophage content following Streptococcus pneumoniae pneumonia in ApoE-deficient mice. Frontiers in Cellular and Infection Microbiology, 13. View this article in WRRO
Al Mansouri M, Patel PA, Chamberlain J & Francis S (2022) OxLDL induces IL-1β release from human EC and VSMC via different caspase-1 dependent mechanisms. Vascular Biology.
Iqbal J, Chamberlain J, Alfaidi M, Hughes M, Alizadeh T, Casbolt H, Evans P, Mann B, Motterlini R, Francis S & Gunn J (2021) Carbon monoxide releasing molecule A1 reduces myocardial damage after acute myocardial infarction in a porcine model. Journal of Cardiovascular Pharmacology, Publish Ahead of Print.
Alfaidi MA, Chamberlain J, Rothman A, Crossman D, Villa‐Uriol MC, Hadoke P, Wu J, Schenkel T, Evans PC & Francis SE (2018) Dietary Docosahexaenoic Acid Reduces Oscillatory Wall Shear Stress, Atherosclerosis, and Hypertension, Most Likely Mediated via an IL‐1–Mediated Mechanism. Journal of the American Heart Association, 7(13). View this article in WRRO
Renshall L, Arnold N, West L, Braithwaite A, Pickworth J, Walker R, Alfaidi M, Chamberlain J, Casbolt H, Thompson AAR , Holt C et al (2018) Selective improvement of pulmonary arterial hypertension with a dual ET A /ET B receptors antagonist in the apolipoprotein E −/− model of PAH and atherosclerosis. Pulmonary Circulation, 8(1), 1-11. View this article in WRRO
Iqbal J, Chamberlain J, Francis SE & Gunn J (2016) Role of Animal Models in Coronary Stenting. Annals of Biomedical Engineering, 44(2), 453-465.
Alfaidi M, Wilson H, Daigneault M, Burnett A, Ridger V, Chamberlain J & Francis SE (2015) Neutrophil Elastase promotes Interleukin-1β secretion from Human Coronary Endothelium. Journal of Biological Chemistry, 290, 24067-24078. View this article in WRRO
Alfaidi M, Wilson H, Ridger V, Chamberlain J & Francis S (2014) Neutrophil elastase enhances IL-1 beta secretion from coronary endothelium via an endolysosomal mediated mechanism, without caspase-1 activation. ANGIOGENESIS, 17(4), 941-941.
Alfaidi M, Chamberlain J & Francis S (2014) 212 Docosahexaenoic Acid but not Alpha-linolenic Acid Ameliorates High Fat Diet Induced Atherosclerosis and Hypertension in APOE-/- MICE Via IL-1 Mechanism.. Heart, 100 Suppl 3, A116-A117.
Dénes Á, Pradillo JM, Drake C, Sharp A, Warn P, Murray KN, Rohit B, Dockrell DH, Chamberlain J, Casbolt H , Francis S et al (2014) Streptococcus pneumoniae worsens cerebral ischemia via interleukin 1 and platelet glycoprotein Ibα.. Ann Neurol, 75(5), 670-683. View this article in WRRO
Alfaidi M, Wilson HL, Burnett A, Abdul-Aema BH, Ridger V, Chamberlain J & Francis SE (2014) 2 IL-1B Secretion in Coronary Vascular Endothelium is Mediated by Neutrophil Serine Protease and is Independent of caspase-1.. Heart, 100 Suppl 1, A2.
Hameed AG, Arnold ND, Chamberlain J, Pickworth JA, Paiva C, Dawson S, Cross S, Long L, Zhao L, Morrell NW , Crossman DC et al (2012) Inhibition of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) reverses experimental pulmonary hypertension.. J Exp Med, 209(11), 1919-1935. View this article in WRRO
Denes A, Drake C, Stordy J, Chamberlain J, McColl BW, Gram H, Crossman D, Francis S, Allan SM & Rothwell NJ (2012) Interleukin-1 Mediates Neuroinflammatory Changes Associated With Diet-Induced Atherosclerosis. JOURNAL OF THE AMERICAN HEART ASSOCIATION, 1(3). View this article in WRRO
Ellam T, Wilkie M, Chamberlain J, Crossman D, Eastell R, Francis S & Chico TJA (2011) Dietary phosphate modulates atherogenesis and insulin resistance in apolipoprotein E knockout mice--brief report.. Arterioscler Thromb Vasc Biol, 31(9), 1988-1990.
Watt V, Chamberlain J, Steiner T, Francis S & Crossman D (2011) TRAIL attenuates the development of atherosclerosis in apolipoprotein E deficient mice.. Atherosclerosis, 215(2), 348-354.
Lawrie A, Hameed AG, Chamberlain J, Arnold N, Kennerley A, Hopkinson K, Pickworth J, Kiely DG, Crossman DC & Francis SE (2011) Paigen diet-fed apolipoprotein E knockout mice develop severe pulmonary hypertension in an interleukin-1-dependent manner.. Am J Pathol, 179(4), 1693-1705. View this article in WRRO
Watt V, Chamberlain J, Steiner T, Francis S & Crossman D (2011) TRAIL attenuates the development of atherosclerosis in apolipoprotein E deficient mice. Atherosclerosis, 215(2), 348-354.
Chamberlain J, Wheatcroft M, Arnold N, Lupton H, Crossman DC, Gunn J & Francis S (2010) A novel mouse model of in situ stenting.. Cardiovasc Res, 85(1), 38-44. View this article in WRRO
Francis S, Chamberlain J, Brookes Z, Shaw G, Alp N, Rogus J, Kornman K & Crossman D (2009) Abstract: 1509 IL-1 MODULATES BLOOD PRESSURE IN MICE AND MAN. Atherosclerosis Supplements, 10(2), e463-e463.
Evans DJW, Jackman LE, Chamberlain J, Crosdale DJ, Judge HM, Jetha K, Norman KE, Francis SE & Storey RF (2009) Platelet P2Y(12) receptor influences the vessel wall response to arterial injury and thrombosis.. Circulation, 119(1), 116-122.
Chamberlain J, Francis S, Brookes Z, Shaw G, Graham D, Alp NJ, Dower S & Crossman DC (2009) Interleukin-1 regulates multiple atherogenic mechanisms in response to fat feeding.. PLoS One, 4(4), e5073. View this article in WRRO
Chamberlain J, Evans D, King A, Dewberry R, Dower S, Crossman D & Francis S (2006) Interleukin-1beta and signaling of interleukin-1 in vascular wall and circulating cells modulates the extent of neointima formation in mice.. Am J Pathol, 168(4), 1396-1403.
Chamberlain J, Dower S, Crossman D & Francis S (2004) INHIBITION OF INTERLEUKIN-1 SIGNALLING REDUCES THE NEOINTIMAL RESPONSE TO ARTERIAL INJURY AND PROMOTES VESSEL REMODELLING. Cardiovascular Pathology, 13(3), 116-117.
Evans D, Crossman D, Francis S & Chamberlain J (2004) TGF-BETA ACTIVATION AND NEOINTIMAL FORMATION IN A MURINE MODEL OF VASCULAR INJURY. Cardiovascular Pathology, 13(3), 117-117.
Chico TJ, Chamberlain J, Gunn J, Arnold N, Bullens SL, Gadek TR, Francis SE, Bunting S, Horton M, Shepherd L , Lipari MT et al (2001) Effect of selective or combined inhibition of integrins alpha(IIb)beta(3) and alpha(v)beta(3) on thrombosis and neointima after oversized porcine coronary angioplasty.. Circulation, 103(8), 1135-1141.
Chamberlain J (2001) Transforming growth factor-beta: a promising target for anti-stenosis therapy.. Cardiovasc Drug Rev, 19(4), 329-344.
Lawrie A, Brisken AF, Francis SE, Tayler DI, Chamberlain J, Crossman DC, Cumberland DC & Newman CM (1999) Ultrasound enhances reporter gene expression after transfection of vascular cells in vitro.. Circulation, 99(20), 2617-2620.
Malik N, Francis SE, Holt CM, Gunn J, Thomas GL, Shepherd L, Chamberlain J, Newman CM, Cumberland DC & Crossman DC (1998) Apoptosis and cell proliferation after porcine coronary angioplasty.. Circulation, 98(16), 1657-1665.
Lawrie A, Brisken AF, Chamberlain JC, Francis SE, Tayler DI, Crossman DC, Cumberland DC & Newman CM (1998) 1MHz ultrasound enhances transfection of porcine aortic endothelial cells in vitro and oligonucleotide uptake in an organ culture model.. AMERICAN JOURNAL OF CARDIOLOGY, 82(7A), 29S-29S.

Conference proceedings papers

Almansouri M, Francis S & Chamberlain J (2018) 140 Acetylated ldl induces IL-1β release from human coronary endothelial cells. Heart, Vol. 104(Suppl 6) (pp A102-A103), 4 June 2018 - 6 June 2018. View this article in WRRO
Alfaidi M, Hughes M, Alizadeh T, Casbolt H, Chamberlain J, Mann BE, Francis SE, Iqbal J & Gunn J (2016) 160 Modulation of Myocardial Ischaemic Injury by Carbon Monoxide Releasing Molecule-A1 in a Porcine Model of Acute Reperfusion Infarction. Heart, Vol. 102(Suppl 6) (pp A114.2-A114), 6 June 2016 - 8 August 2016.
Alfaidi M, Schenkel T, Evans P, Chamberlain J & Francis S (2015) 192 Dietary Docosahexaenoic Acid Reduced Experimental Atherosclerosis by Inducing Protective Haemodynamic Conditions. Heart, Vol. 101(Suppl 4) (pp A107.2-A107)
Alfaidi M, Wilson H, Burnett A, Chamberlain J & Francis S (2013) IL-1 BETA SECRETION IN CORONARY VASCULAR ENDOTHELIUM IS MEDIATED BY NEUTROPHIL SERINE PROTEASE AND IS INDEPENDENT OF CASPASE-1. HEART, Vol. 99 (pp A99-A99)
Hameed AG, Arnold ND, Chamberlain J, Pickworth JA, Paiva C, Dawson S, Cross S, Long L, Zhao L, Morrell NW , Crossman DC et al (2013) Tumour necrosis factor-related apoptosis-inducing ligand is a novel therapeutic target in pulmonary arterial hypertension. The Lancet, Vol. 381, Supplement 1 (pp S47 --S47 -)
Hameed AG, Arnold ND, Chamberlain J, Pickworth J, Newman CMH, Crossman DC, Francis SE & Lawrie A (2012) TISSUE TRAIL DRIVES PULMONARY VASCULAR REMODELING AND ITS INHIBITION REVERSES EXPERIMENTAL PULMONARY ARTERIAL HYPERTENSION. HEART, Vol. 98 (pp A1-A1)
Lawrie A, Hameed AG, Arnold N, Chamberlain J, Pickworth J, Dawson S, Newman CMH & Francis SE (2012) Osteoprotegerin Blockade Prevents And Reverses Experimental Pulmonary Arterial Hypertension. D97. PULMONARY HYPERTENSION: NEW INSIGHTS INTO CAUSES AND TREATMENTS?
Hameed AG, Arnold ND, Pickworth J, Chamberlain JC, Newman CMH, Crossman DC, Francis SE & Lawrie A (2011) TRAIL IS A POTENTIAL NOVEL THERAPEUTIC TARGET IN PULMONARY ARTERIAL HYPERTENSION. THORAX, Vol. 66 (pp A3-A3)
Lawrie A, Hameed AG, Chamberlain J, Arnold N, Crossman DC & Francis SE (2011) PAIGEN DIET-FED APOLIPOPROTEIN E KNOCK-OUT MICE DEVELOP SEVERE PULMONARY HYPERTENSION IN AN INTERLEUKIN-1 DEPENDENT MANNER. HEART, Vol. 97(20) (pp 10-10)
Hameed AG, Chamberlain J, Arnold N, Newman C, Francis S, Crossman D & Lawrie A (2011) Beneficial Effects Of Inhibiting Trail In Monocrotaline-Induced Experimental Pulmonary Hypertension. B61. PULMONARY HYPERTENSION: EXPERIMENTAL MODELS I
Hameed AG, Chamberlain J, Arnold ND, Francis SE, Newman CMH, Crossman DC & Lawrie A (2010) TRAIL DEFICIENCY IS PROTECTIVE IN EXPERIMENTAL PULMONARY ARTERIAL HYPERTENSION. THORAX, Vol. 65 (pp A68-A68)
Francis S, Chamberlain J, Brookes Z, Shaw G, Alp N, Rogus J, Kornman K & Crossman D (2009) IL-1 MODULATES BLOOD PRESSURE IN MICE AND MAN. ATHEROSCLEROSIS SUPP, Vol. 10(2)
Francis S, Chamberlain J, Brookes Z, Shaw G, Alp N, Rogus J, Kornman K & Crossman D (2009) IL-1 MODULATES BLOOD PRESSURE IN MICE AND MAN. ATHEROSCLEROSIS SUPPLEMENTS, Vol. 10(2). Boston, MA, 14 June 2009 - 18 June 2009.
Chamberlain J, Wheatcroft M, Arnold N, Francis S, Lupton H & Gunn J (2009) A NOVEL MURINE MODEL OF STENTING. HEART, Vol. 95 (pp A37-A38)
Evans DJW, Crosdale DJ, Judge HM, Chamberlain J, Jetha K, Norman KE, Francis SE & Storey RF (2008) The platelet P2Y12 receptor influences the vessel wall response to arterial injury and thrombosis. JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, Vol. 51(10) (pp A291-A291)
Townsend CF, Francis SE, Chamberlain J, Gunn J, Crossman DC & Newman CMH (2001) Modulation of MSS1, a component of the 26S proteasome, in vascular smooth muscle cells in vitro and following experimental PTCA. EUR HEART J, Vol. 22 (pp 3-3)
Chamberlain J, Gunn J, Francis SE, Holt CM, Cumberland DC & Crossman DC (1998) IL-1 beta synthesis and neovascularisation following porcine coronary arterial injury. J VASC RES, Vol. 35(5) (pp 389-389)
Gunn J, Francis S, Holt C, Chamberlain J, Lambert D, Newman C, Crossman D & Cumberland D (1997) Is local drug delivery truly a low efficiency procedure?. AM J CARDIOL, Vol. 80 (pp TC240-TC240)
Alfaidi MAA, Wilson H, Ridger, V, Chamberlain J & Francis S () Neutrophil elastase enhances IL-1 beta secretion from coronary endothelium via an endolysosomal mediated mechanism, without caspase-1 activation. ANGIOGENESIS, Vol. 17(4) (pp 941-941). California, USA, 18 October 2014 - 23 October 2014.

Preprints

Bazaz R, Marriott H, Wright C, Chamberlain J, West L, Gelsthorpe C, Heath P, Maleki-Dizaji A, Francis S & Dockrell D () Transient increase in atherosclerotic plaque macrophage content following Streptococcus pneumoniae pneumonia in ApoE-deficient mice, Research Square Platform LLC.

Teaching interests

I teach on several undergraduate and postgraduate taught modules, including MBB334 Biochemical Basis of Human Disease, CDL301 Cardiovascular Pharmacology: Personalising Medicine, several modules within both the MSc in Molecular Medicine, and within the CDLT01 MRes Cardiovascular Medicine: From Molecules to Man.

I am the module co-ordinator for CDL301/6301, module lead CDL601 and CDL602 (Research Skills), deputy module lead for CDL601 (Literature Review) and CDL401 (Vascular Cell Biology), and occasionally deputise for the overall programme of CDLT01 MRes Cardiovacular Medicine:From Molecules to Man. In addition, I also am responsible for the Blackboard site, for CDL302 (Pharmacology of Respiratory Disease).

I am Deputy Director of Learning & Teaching for the Department. I am Chair of the Department’s Undergraduate Teaching Committee, and am a member of the MRes Committee. I have a particular interest in supporting students with an SpLD, and actively engage in delivering CPD to staff with respect to the use of new teaching technologies and ensuring their teaching is inclusive to all students.