Professor Chris Toseland

All publications

Journal articles

• Gawor M, Lehka L, Lambert D & Toseland CP (2025) Actin from within – how nuclear myosins and actin regulate nuclear architecture and mechanics. Journal of Cell Science, 138(3). View this article in WRRO
• dos Santos A, Rollins DE, Hari-Gupta Y, Reed HCW, Du M, Ru SYZ, Pidlisna K, Stranger A, Lorgat F, Lambert D , Brown I et al (2023) Autophagy receptor NDP52 alters DNA conformation to modulate RNA polymerase II transcription. Nature Communications, 14. View this article in WRRO
• Shahid-Fuente IW & Toseland CP (2023) Myosin in chromosome organisation and gene expression. Biochemical Society Transactions, 51(3), 1023-1034. View this article in WRRO
• dos Santos Á, Fili N, Hari-Gupta Y, Gough RE, Wang L, Martin-Fernandez M, Aaron J, Wait E, Chew T-L & Toseland CP (2022) Binding partners regulate unfolding of myosin VI to activate the molecular motor. Biochemical Journal, 479(13), 1409-1428.
• dos Santos Á, Rehfeldt F & Toseland CP (2022) Measuring Nuclear Mechanics with Atomic Force Microscopy, 171-181.
• dos Santos Á, Gough RE, Wang L & Toseland CP (2022) Measuring Nuclear Organization of Proteins with STORM Imaging and Cluster Analysis, 293-309.
• Hari-Gupta Y, Fili N, dos Santos Á, Cook AW, Gough RE, Reed HCW, Wang L, Aaron J, Venit T, Wait E , Grosse-Berkenbusch A et al (2022) Myosin VI regulates the spatial organisation of mammalian transcription initiation. Nature Communications, 13(1).
• dos Santos A & Toseland C (2022) Magnetic tweezers in a microplate format. Journal of Visualized Experiments, 180. View this article in WRRO
• dos Santos Á & Toseland CP (2021) Regulation of nuclear mechanics and the impact on DNA damage. International Journal of Molecular Sciences, 22(6).
• Cook AW & Toseland CP (2021) The roles of nuclear myosin in the DNA damage response. The Journal of Biochemistry, 169(3), 265-271.
• Santos ÁD, Fili N, Pearson DS, Hari-Gupta Y & Toseland CP (2021) High throughput mechanobiology : force modulation of ensemble biochemical and cell-based assays. Biophysical Journal, 120(4), 631-641.
• Emmanouilidis I, Fili N, Cook AW, Hari-Gupta Y, dos Santos Á, Wang L, Martin-Fernandez ML, Ellis PJI & Toseland CP (2021) A targeted and tuneable DNA damage tool using CRISPR/Cas9. Biomolecules, 11(2).
• dos Santos A, Cook A, Gough R, Schilling M, Aleida Olszok N, Brown I, Wang L, Aaron J, Martin-Fernandez M, Rehfeldt F & Toseland C (2021) DNA damage alters nuclear mechanics through chromatin reorganization. Nucleic Acids Research, 49(1), 340-353. View this article in WRRO
• Cook AW, Gough RE & Toseland CP (2020) Nuclear myosins – roles for molecular transporters and anchors. Journal of Cell Science, 133(11).
• Fili N, Hari-Gupta Y, Aston B, dos Santos Á, Gough RE, Alamad B, Wang L, Martin-Fernandez ML & Toseland CP (2020) Competition between two high- and low-affinity protein-binding sites in myosin VI controls its cellular function.. Journal of Biological Chemistry, 295(2), 337-347.
• Acton SE, Bell AJD, Toseland CP & Twelvetrees A (2019) Research culture : a survey of new PIs in the UK. eLife, 2019(8). View this article in WRRO
• Fili N, Hari-Gupta Y, Aston B, dos Santos Á, Gough RE, Alamad B, Wang L, Martin-Fernandez M & Toseland CP (2019) Binding partner regulation of Myosin VI: Loss of tumour-suppressor Dab2 leads to enhanced activity of nuclear myosin.
• Cook A, Hari-Gupta Y & Toseland CP (2018) Application of the SSB biosensor to study in vitro transcription. Biochemical and Biophysical Research Communications, 496(3), 820-825.
• Fili N, Hari-Gupta Y, dos Santos Á, Cook A, Poland S, Ameer-Beg SM, Parsons M & Toseland CP (2017) NDP52 activates nuclear myosin VI to enhance RNA polymerase II transcription. Nature Communications, 8(-), ---.
• Toseland C (2017) Function of Nuclear Myosin Motors: A Biochemical and Single Molecule Characterization - MRC. Impact, 2017(10), 49-51.
• Lherbette M, dos Santos Á, Hari-Gupta Y, Fili N, Toseland CP & Schaap IAT (2017) Atomic force microscopy micro-rheology reveals large structural inhomogeneities in single cell-nuclei. Scientific Reports, 7.
• Cook A, Hari-Gupta Y & Toseland CP (2017) A reagentless biosensor for mRNA: a new tool to study transcription.
• Yao M, Goult BT, Klapholz B, Hu X, Toseland CP, Guo Y, Cong P, Sheetz MP & Yan J (2016) The mechanical response of talin. Nature Communications, 7.
• Wilhelm L, Buermann F, Minnen A, Shin H-C, Toseland CP, Oh B-H & Gruber S (2015) SMC condensin entraps chromosomal DNA by an ATP hydrolysis dependent loading mechanism in Bacillus subtilis. eLife, 4. View this article in WRRO
• Kang H-A, Shin H-C, Kalantzi A-S, Toseland CP, Kim H-M, Gruber S, Dal Peraro M & Oh B-H (2015) Crystal structure of Hop2-Mnd1 and mechanistic insights into its role in meiotic recombination. Nucleic Acids Research, 43(7), 3841-3856. View this article in WRRO
• Soh Y-M, Buermann F, Shin H-C, Oda T, Jin KS, Toseland CP, Kim C, Lee H, Kim SJ, Kong M-S , Durand-Diebold M-L et al (2015) Molecular basis for SMC rod formation and its dissolution upon DNA binding. Molecular Cell, 57(2), 290-303. View this article in WRRO
• Toseland CP & Geeves MA (2014) Rapid Reaction Kinetic Techniques, 49-65.
• Fili N & Toseland CP (2014) Fluorescence and Labelling: How to Choose and What to Do, 1-24.
• Toseland CP (2014) Fluorescence to Study the ATPase Mechanism of Motor Proteins, 67-86.
• Toseland CP & Webb MR (2013) ATPase Mechanism of the 5′-3′ DNA Helicase, RecD2. Journal of Biological Chemistry, 288(35), 25183-25193.
• Toseland CP (2013) Fluorescent labeling and modification of proteins. Journal of Chemical Biology, 6(3), 85-95.
• Chisty LT, Toseland CP, Fili N, Mashanov GI, Dillingham MS, Molloy JE & Webb MR (2013) Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD. Nucleic Acids Research, 41(9), 5010-5023.
• Batters C, Woodall KA, Toseland CP, Hundschell C & Veigel C (2012) Cloning, Expression, and Characterization of a Novel Molecular Motor, Leishmania Myosin-XXI. Journal of Biological Chemistry, 287(33), 27556-27566.
• Batters C, Woodall K, Toseland C & Veigel C (2012) Parasite Motility: Mechanisms of a Novel Molecular Motor, Myosin XXI. Biophysical Journal, 102(3), 570a-570a.
• Toseland CP & Webb MR (2011) Fluorescent Nucleoside Triphosphates for Single-Molecule Enzymology, 161-174.
• Fili N, Toseland CP, Dillingham MS, Webb MR & Molloy JE (2011) A Single-Molecule Approach to Visualize the Unwinding Activity of DNA Helicases, 193-214.
• Fili N, Mashanov GI, Toseland CP, Batters C, Wallace MI, Yeeles JTP, Dillingham MS, Webb MR & Molloy JE (2010) Visualizing helicases unwinding DNA at the single molecule level. Nucleic Acids Research, 38(13), 4448-4457.
• Toseland CP & Webb MR (2010) Fluorescence tools to measure helicase activity in real time. Methods, 51(3), 259-268.
• Toseland CP, Martinez-Senac MM, Slatter AF & Webb MR (2009) The ATPase Cycle of PcrA Helicase and Its Coupling to Translocation on DNA. Journal of Molecular Biology, 392(4), 1020-1032.
• Davies MN, Guan P, Blythe MJ, Salomon J, Toseland CP, Hattotuwagama C, Walshe V, Doytchinova IA & Flower DR (2007) Using databases and data mining in vaccinology. Expert Opinion on Drug Discovery, 2(1), 19-35.
• Taylor PD, Toseland CP, Attwood TK & Flower DR (2006) Alpha helical trans-membrane proteins: Enhanced prediction using a Bayesian approach.. Bioinformation, 1(6), 234-236.
• Taylor PD, Toseland CP, Attwood TK & Flower DR (2006) A predictor of membrane class: Discriminating alpha-helical and beta-barrel membrane proteins from non-membranous proteins.. Bioinformation, 1(6), 208-213.
• Taylor PD, Toseland CP, Attwood TK & Flower DR (2006) Beta barrel trans-membrane proteins: Enhanced prediction using a Bayesian approach.. Bioinformation, 1(6), 231-233.
• Hattotuwagama CK, Toseland CP, Guan P, Taylor DJ, Hemsley SL, Doytchinova IA & Flower DR (2006) Toward Prediction of Class II Mouse Major Histocompatibility Complex Peptide Binding Affinity: In Silico Bioinformatic Evaluation Using Partial Least Squares, a Robust Multivariate Statistical Technique.. ChemInform, 37(31).
• Davies MN, Toseland CP, Moss DS & Flower DR (2006) Benchmarking pKa prediction. BMC Biochemistry, 7(1).
• Toseland CP (2006) PPD v1.0--an integrated, web-accessible database of experimentally determined protein pKa values. Nucleic Acids Research, 34(90001), D199-D203.
• Hattotuwagama CK, Toseland CP, Guan P, Taylor DJ, Hemsley SL, Doytchinova IA & Flower DR (2006) Toward Prediction of Class II Mouse Major Histocompatibility Complex Peptide Binding Affinity:  in Silico Bioinformatic Evaluation Using Partial Least Squares, a Robust Multivariate Statistical Technique. Journal of Chemical Information and Modeling, 46(3), 1491-1502.
• Toseland CP, Clayton DJ, McSparron H, Hemsley SL, Blythe MJ, Paine K, Doytchinova IA, Guan P, Hattotuwagama CK & Flower DR (2005) . Immunome Research, 1(1), 4-4.
• Guan P, Davies M, Taylor D, Wan S, McSparron H, Hemsley S, Toseland C, Blythe M, Taylor P, Walshe V , Hattotuwagama C et al (2005) Computational Chemistry, Informatics, and the Discovery of Vaccines. Current Computer Aided-Drug Design, 1(4), 377-395.
• Fili N & Toseland CP () Unconventional Myosins: How Regulation Meets Function. International Journal of Molecular Sciences, 21(1), 67-67.
• Toseland CP, Powell B & Webb MR () ATPase Cycle and DNA Unwinding Kinetics of RecG Helicase. PLoS ONE, 7(6), e38270-e38270.
• Taylor PD, Toseland CP, Attwood TK & Flower DR () TATPred: a Bayesian method for the identification of twin arginine translocation pathway signal sequences. Bioinformation, 1(5), 184-187.
• Taylor PD, Toseland CP, Attwood TK & Flower DR () LIPPRED: A web server for accurate prediction of lipoprotein signal sequences and cleavage sites. Bioinformation, 1(5), 176-179.
• Toseland CP, McSparron HM & Flower DR () DSD – An integrated, web-accessible database of Dehydrogenase Enzyme Stereospecificities. BMC Bioinformatics, 6(1).

Book chapters

• Webb MR & Toseland CP (2013) Helicases, Encyclopedia of Biophysics (pp. 950-958). Springer Berlin Heidelberg
• Fili N, Toseland CP, Dillingham MS, Webb MR & Molloy JE (2011) Erratum to: A Single-Molecule Approach to Visualize the Unwinding Activity of DNA Helicases, Methods in Molecular Biology (pp. E1-E1). Humana Press
• Hattotuwagama CK, Guan P, Davies M, Taylor DJ, Walshe V, Hemsley SL, Toseland C, Doytchinova IA, Borrow P & Flower DR () Empirical, AI, and QSAR Approaches to Peptide-MHC Binding Prediction, In Silico Immunology (pp. 139-175). Springer US

Conference proceedings

• Cook A, Hari Gupta Y, Venit T, Percipalle P & Toseland CP (2019) Myosin VI and its Role in the DNA Damage Response. Biophysical Journal, Vol. 116(3) (pp 77a-77a)
• dos Santos A, Wang L & Toseland CP (2019) Nuclear Ndp52 - a Putative Transcription Regulator. Biophysical Journal, Vol. 116(3) (pp 210a-210a)
• Fili N, Cook A, Hari Gupta Y & Toseland CP (2019) Novel Tales About the Myosin VI Tail. Biophysical Journal, Vol. 116(3) (pp 178a-178a)
• Gough RE, Fili N, Hari Gupta Y & Toseland CP (2019) Deciphering Load-Induced Anchoring by Myosin VI. Biophysical Journal, Vol. 116(3) (pp 260a-260a)
• Hari Gupta Y, Fili N, dos Santos A, Chew T-L, Aaron J, Wang L & Toseland CP (2019) Nuclear Myosin VI Stabilizes RNA Polymerase II in Transcription Factories. Biophysical Journal, Vol. 116(3) (pp 208a-209a)
• Hari Gupta Y, Fili N, dos Santos A, Chew T-L, Aaron J, Wang L & Toseland CP (2019) Nuclear Myosin VI Regulates Estrogen Receptor Driven Gene Expression. Biophysical Journal, Vol. 116(3) (pp 209a-209a)
• Fili N, dos Santos A, Hari-Gupta Y & Toseland CP (2016) Activation of nuclear myosin VI leads to enhanced gene expression.. MOLECULAR BIOLOGY OF THE CELL, Vol. 27
• Fili N, Hari-Gupta Y & Toseland CP (2016) Dissecting the role of Myosin VI in the nucleus.. MOLECULAR BIOLOGY OF THE CELL, Vol. 27
• Toseland CP, Ellrich H, Fili N, Batters C & Veigel C (2012) Myosin VI Comes Together - Structure and Motor Regulation by Binding Partners. Biophysical Journal, Vol. 102(3) (pp 569a-569a)
• Veigel C, Batters C, Toseland CP & Sellers JR (2011) Direct Observation of the Myosin Power Stroke and its Reversal. Biophysical Journal, Vol. 100(3) (pp 116a-116a)
• Toseland CP, Batters C & Veigel C (2011) Single Molecule Fluorescence and Force Measurements on PcrA Helicase. Biophysical Journal, Vol. 100(3) (pp 72a-73a)
• Toseland CP & Webb MR (2009) PcrA Helicase ATPase Mechanism: RepD Modulation During Unwinding. Biophysical Journal, Vol. 96(3) (pp 415a-415a)

Preprints

• González-Novo R, Zamora-Carreras H, Lope-Planelles AD, López-Menéndez H, Roda-Navarro P, Monroy F, Wang L, Toseland CP & Redondo Muñoz J (2023) 3D environment promotes persistent changes in lamin B1 distribution, the biomechanical signature of the nucleus, and adaptative survival and migratory functions, Cold Spring Harbor Laboratory.
• dos Santos Á, Rollins DE, Hari-Gupta Y, Reed HCW, Du M, Ru SYZ, Pidlisna K, Stranger A, Lorgat F, Brown I , Howland K et al (2022) Autophagy receptor NDP52 alters DNA conformation to modulate RNA Polymerase II transcription, Cold Spring Harbor Laboratory.
• Emmanouilidis I, Fili N, Cook AW, Hari-Gupta Y, dos Santos Á, Wang L, Martin-Fernandez M, Ellis PJI & Toseland CP (2021) A targeted and tuneable DNA damage tool using CRISPR/Cas9, Cold Spring Harbor Laboratory.
• dos Santos Á, Cook AW, Gough RE, Schilling M, Olszok NA, Brown I, Wang L, Aaron J, Martin-Fernandez ML, Rehfeldt F & Toseland CP (2020) DNA damage alters nuclear mechanics through chromatin reorganisation, Cold Spring Harbor Laboratory.
• dos Santos Á, Fili N, Hari-Gupta Y, Gough RE, Wang L, Martin-Fernandez M, Aaron J, Wait E, Chew T-L & Toseland CP (2020) Binding partners regulate unfolding of myosin VI to activate the molecular motor, Cold Spring Harbor Laboratory.
• Santos ÁD, Fili N, Pearson DS, Hari-Gupta Y & Toseland CP (2020) High throughput mechanobiology: Force modulation of ensemble biochemical and cell-based assays, Cold Spring Harbor Laboratory.
• Hari-Gupta Y, Fili N, dos Santos Á, Cook AW, Gough RE, Reed HCW, Wang L, Aaron J, Venit T, Wait E , Grosse-Berkenbusch A et al (2020) Nuclear myosin VI regulates the spatial organization of mammalian transcription initiation, Cold Spring Harbor Laboratory.
• Große-Berkenbusch A, Hettich J, Kuhn T, Fili N, Cook AW, Hari-Gupta Y, Palmer A, Streit L, Ellis PJI, Toseland CP & Gebhardt JCM (2020) Myosin VI moves on nuclear actin filaments and supports long-range chromatin rearrangements, Cold Spring Harbor Laboratory.