Microsatellite libraries
NEOF has supported the isolation of markers from 125 species:
Off
| 39 birds | 28 insects | 22 mammals |
| 7 flowering plants | 7 fishes | 6 amphibians |
| 6 reptiles | 2 crustaceans | 2 molluscs |
| 4 other invertebrates | 1 cephalopod | 1 algae |
Microsatellite libraries created
Mammals
- Snow leopard, Panthera uncial (Miseq)
- African golden wolf, Canis anthus (Miseq)
- Spotted hyena, Crocuta crocuta
- Eurasian badger, Meles meles
- Pine marten, Martes martes (Miseq)
- American mink, Neovison vison (Miseq)
- Rabbit, Oryctolagus cuniculus
- Royal pika, Ochotona roylei (Miseq)
- Milne-Edwards´ sportive lemur, Lepilemur edwardsi
- American red squirrel, Tamiasciurus hudsonicus
- Indochinese flying squirrel, Hylopetes phayrei
- Berdmore's ground squirrel, Menetes berdmorei
- Gray-cheeked flying squirrel, Hylopetes lepidus
- Greater horseshoe bat, Rhinolophus ferrumequinum
- Papillose woolly bat, Kerivoula papillosa
- Fawn leaf-nosed bat, Hipposideros cervinus
- Grey long-eared bat, Plecotus austriacus (454)
- Conserved markers for Myotis bats
- Water shrew, Neomys fodiens
- Pyreanean desman, Galemys pyrenaicus (Miseq)
- Egyptian spiny mouse, Acomys dimiditus (454)
- Hazel dormouse, Muscardinus avellanarius (454)
Birds
Passerines:
- House sparrow, Passer domesticus
- Zebra finch, Taeniopygia guttata (isolated via genome sequence)
- Gouldian finch, Chloebia gouldiae
- Blue tit, Parus caeruleus
- Seychelles warbler, Acrochephalus sechellensis
- Great reed warbler, Acrocephalus arundinaceus
- Black-billed magpie, Pica pica
- Azure-winged magpie, Cyanopica cyanus
- Superb fairy-wren, Malurus cyaneus
- Reed bunting, Emberiza schoeniclus
- Hihi (Stitchbird), Notiomystis cincta
- Seychelles flycatcher, Terpsiphone corvina
- Rifleman, Acanthisitta chloris
- European stonechat, Saxicola rubicola (MiSeq)
- European bee-eater, Merops apiaster (Miseq)
Non-passerines:
- Great spotted cuckoo, Clamator glandarius
- Australian brush-turkey, Alectura lathami
- Chicken, Gallus gallus
- Light bellied Brent goose, Branta bernicla hrota
- Ostrich, Struthio camelus
- Mauritius pink pigeon Columba mayeri
- Ruff, Philomachus pugnax
- Whiskered auklet, Aethia pygmaea
- Kentish plover, Charadrius alexandrinus
- Leach's storm petrel, Oceanodroma leucorhoa
- Round Island petrel, hybrid Pterodroma arminjoniana spp (454)
- Herald petrel, Pterodroma heraldica (454)
- Macaroni penguin, Eudyptes chrysolophus
- Sooty tern, Onychoprion fuscatus (Miseq)
- Great bustard, Otis tarda
- Stone curlew, Burhinus oedicnemus
- Common pheasant, Phasianus colchicus (Miseq)
- Monk parakeet, Myiopsitta monachus (Miseq)
Birds of prey:
- Osprey, Pandion haliaetus
- Turkey vulture, Cathartes aura
- Black vulture, Coragyps atratus
- Reunion harrier, Circus maillardi
- Eagle owl, Bubo bubo
- Pygmy falcon, Polihierax semitorquatus (Miseq)
Fishes
- Bitterling, Rhodeus sericeus
- Icefish (Mackerel icefish), Champsocephalus gunnari
- Notothenia rossi (no common name)
- Basking shark, Cetorhinus maximus
- Flapper skate, Dipturus intermedia (Miseq)
- Blue skate, Dipturus flossada (Miseq)
- Porbeagle shark, Lamna nasus (Miseq)
Reptiles
- Skink, Gongylomorphus (4 species)
- Bojer's skink, Gongylomorphus bojerii
- Orange tailed skink, Gongylomorphus spp
- Wall lizard, Podarcis muralis (454)
- Mauritius lowland day gecko, Phelsuma guimbeaui guimbeau
Amphibians
- Crested newt, Triturus cristatus
- Smooth newt, Triturus vulgaris
- Caecilian (Gymnophiona), Boulengerula uluguruenis
- Guatemala spike-thumb frog, Plectrohyla guatemalensis (Miseq)
- Honduras spike-thumb frog, Plectrohyla dasypus (Miseq)
- Copan brook frog, Duellmanohyla soralia (Miseq)
Molluscs
- Common mudsnail, Hydrobia ulvae
- Shining ramshorn snail, Segmentina nitida (Miseq)
Insects
- Tree weta, Hemideina maori
- Field cricket, Gryllus bimaculatus
- Bark beetle sp., Coccotrypes dactyliperda
- Bark beetle sp., Coccotrypes carpophagus
- Net-spinning caddisfly sp., Polycentropus flavomaculatus
- Stalk-eyed fly, Diasemopsis meigenii
- Common bedbug, Cimex lectularius
- Garden tiger moth, Arctia caja
- Apollo butterfly, Parnassius apollo nevadensis
- Parasitic wasp, Microstigmus nigrophthalmus
- Chalcid parasitic oak gallwasp (3 species)
- Heathland solitary wasp, Ammophila pubescens
- Tropical hover wasp, Liostenogaster flavolineata
- Sweat bee, Lasioglossum malachurum
- Red mason bee, Osmia bicornis (isolated via genome sequence)
Mosquito, Aedes taeniorhynchus
Mosquito sp., Sabethes sp.
Dance flies (multiple species)
12 species of dance fly (Diptera:Empididae) across three genera (Rhamphomyia (n=5), Empis(n=4) and Hilara (n=3)): Rhamphomyia longicauda, R. crassirostris, R. tibiella, R. longipes, R. nigripennis, Empis aestiva, E. tessellata, E. nigripes, E. stercorea, Hilara maura, H. chorica, H. interstincta
Crustaceans
- Ostracod, Eucypris virens
- White-clawed crayfish, Austropotamobius pallipes
Cephalopods
- Nautilus, Nautilus pompilius (MiSeq)
Other Invertebrates
- Scab mite, Psoroptes
- Ascidian, Diplosoma listerianum
- Pink sea fan, Eunicella verrucosa
- Dead man's fingers, Alcyonium digitatum
Flowering plants
- Elder-flowered orchid, Dactylorhiza sambucina
- Green-winged orchid, Orchis morio
- African fig, Ficus sycomorus
- Stemless thistle, Cirsium acaule
- Greater water parsnip, Sium latifolium (MiSeq)
- Quaking-grass, Briza media
- Blue sedge, Carex flacca
Algae
- Red alga sp., Gracilariopsis longissima
Sequencing methods
454 - indicates the clones were sequenced using 454 sequencing at the University of Liverpool.
MiSeq - indicates an Illumina paired-end library was created using 1µg of repeat-enriched genomic DNA and by following the standard protocol of the SureSelect Library Prep Kit, ILM (Agilent Technologies Inc. Santa Clara, California) and DNA sequencing was conducted using a MiSeq Benchtop Sequencer (Illumina Inc., San Diego, California) by the Sheffield Diagnostics Genetics Service, Sheffield Children's Hospital.
Other libraries were created using Sanger sequencing and a consensus sequence created from the forward and reverse sequences.