Dr Roy Chaudhuri
School of Biosciences
Lecturer in Bioinformatics
r.chaudhuri@sheffield.ac.uk
+44 114 222 2837
+44 114 222 2837
Firth Court
Full contact details
Dr Roy Chaudhuri
School of Biosciences
Firth Court
Western Bank
Sheffield
S10 2TN
School of Biosciences
Firth Court
Western Bank
Sheffield
S10 2TN
- Research interests
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My research interests are primarily in the area of bacterial genomics.
Current research topics include:
- Comparative genomics and phylogenetics of bacterial pathogens, particularly E. coli and Salmonella
- Use of transposon insertion sequencing methods (TraDIS/TnSeq/HITS/InSeq) to identify essential bacterial genes and genes important for survival in particular environments such as during infection of a model system
- Development of user-friendly software tools and online resources for exploring data from -omics technologies. Examples include coliBASE, Xbase and the recently-funded MicrobesNG.
- Publications
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Show: Featured publications All publications
Featured publications
Journal articles
- Genes Required for the Fitness of Salmonella enterica Serovar Typhimurium during Infection of Immunodeficient gp91 −/− phox Mice. Infection and Immunity, 84(4), 989-997. View this article in WRRO
- Genomic signatures of human and animal disease in the zoonotic pathogen Streptococcus suis. Nature Communications, 6(1). View this article in WRRO
- The use of genome wide association methods to investigate pathogenicity, population structure and serovar in Haemophilus parasuis. BMC Genomics, 15(1), 1179-1179. View this article in WRRO
- Generation of a Tn5 transposon library in Haemophilus parasuis and analysis by transposon-directed insertion-site sequencing (TraDIS).. Vet Microbiol, 166(3-4), 558-566.
- Gene content and diversity of the loci encoding biosynthesis of capsular polysaccharides of the 15 serovar reference strains of Haemophilus parasuis.. J Bacteriol, 195(18), 4264-4273.
- Comprehensive assignment of roles for Salmonella typhimurium genes in intestinal colonization of food-producing animals.. PLoS Genet, 9(4), e1003456. View this article in WRRO
- The evolution of the Escherichia coli phylogeny.. Infect Genet Evol, 12(2), 214-226.
- Quantitative RNA-seq analysis of the Campylobacter jejuni transcriptome.. Microbiology, 157(Pt 10), 2922-2932.
- Retrospective application of transposon-directed insertion site sequencing to a library of signature-tagged mini-Tn5Km2 mutants of Escherichia coli O157:H7 screened in cattle. Journal of Bacteriology, 193(7), 1771-1776.
- A commensal gone bad: complete genome sequence of the prototypical enterotoxigenic Escherichia coli strain H10407.. J Bacteriol, 192(21), 5822-5831.
- Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042.. PLoS One, 5(1), e8801.
- Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).. BMC Genomics, 10, 291. View this article in WRRO
- Comprehensive identification of Salmonella enterica serovar typhimurium genes required for infection of BALB/c mice. PLoS Pathogens, 5(7). View this article in WRRO
- The pangenome structure of Escherichia coli: Comparative genomic analysis of E. coli commensal and pathogenic isolates. Journal of Bacteriology, 190(20), 6881-6893.
- xBASE, a collection of online databases for bacterial comparative genomics.. Nucleic Acids Res, 34(Database issue), D335-D337.
- Escherichia coli K-12: A cooperatively developed annotation snapshot - 2005. Nucleic Acids Research, 34(1), 1-9.
All publications
Journal articles
- Identification of pathways to high-level vancomycin resistance in Clostridioides difficile that incur high fitness costs in key pathogenicity traits. PLOS Biology, 22(8). View this article in WRRO
- Molecular characterization of Streptococcus pyogenes (StrepA) non-invasive isolates during the 2022–2023 UK upsurge. Microbial Genomics, 10(8).
- DUF916 and DUF3324 in the WxL protein cluster bind to WxL and link bacterial and host surfaces. Protein Science, 32(11). View this article in WRRO
- An intact S-layer is advantageous to Clostridioides difficile within the host. PLOS Pathogens, 19(6).
- Genomic characterization of skin and soft tissue Streptococcus pyogenes isolates from a low-income and a high-income setting. mSphere, 8(1).
- Identification of candidate virulence loci in Striga hermonthica, a devastating parasite of African cereal crops.
- Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site. Microbiology, 167(12).
- Rationally designed mariner vectors for functional genomic analysis of Actinobacillus pleuropneumoniae and other Pasteurellaceae species by transposon-directed insertion-site sequencing (TraDIS). Animal Diseases, 1(1).
- Probabilistic identification of bacterial essential genes via insertion density using TraDIS data with Tn5 libraries. Bioinformatics. View this article in WRRO
- Extensive microbial diversity within the chicken gut microbiome revealed by metagenomics and culture. PeerJ, 9. View this article in WRRO
- Architecture and self-assembly of clostridium sporogenes and clostridium botulinum spore surfaces illustrate a general protective strategy across spore formers. mSphere, 5(4). View this article in WRRO
- Increasing prevalence of a fluoroquinolone resistance mutation amongst Campylobacter jejuni isolates from four human infectious intestinal disease studies in the United Kingdom. PLoS ONE, 15(1). View this article in WRRO
- Draft Genome Sequences of the Type Strains of Actinobacillus indolicus (46K2C) and Actinobacillus porcinus (NM319), Two NAD-Dependent Bacterial Species Found in the Respiratory Tract of Pigs. Microbiology Resource Announcements, 9(1).
- Publisher Correction: Genomic signatures of human and animal disease in the zoonotic pathogen Streptococcus suis. Nature Communications, 10(1).
- The fitness landscape of the African salmonella typhimurium ST313 strain D23580 reveals unique properties of the pBT1 plasmid. PLOS Pathogens, 15(9). View this article in WRRO
- Draft Whole-Genome Sequences of 10 Aeromonas Strains from Clinical and Environmental Sources. Microbiology Resource Announcements, 8(30). View this article in WRRO
- Proteomic profiling, transcription factor modeling, and genomics of evolved tolerant strains elucidate mechanisms of vanillin toxicity in Escherichia coli.. mSystems, 4(4). View this article in WRRO
- Applying transposon-directed insertion site sequencing to industrially important, solventogenic species Clostridium saccharoperbutylacetonicum. Access Microbiology, 1(1A).
- Adding function to the genome of African Salmonella Typhimurium ST313 strain D23580. PLoS Biology, 17(1). View this article in WRRO
- Retrospective application of transposon-directed insertion-site sequencing to investigate niche-specific virulence of Salmonella Typhimurium in cattle. BMC Genomics, 20. View this article in WRRO
- Use of proteins identified through a functional genomic screen to develop a protein subunit vaccine that provides significant protection against virulent Streptococcus suis in pigs. Infection and Immunity, 86(3). View this article in WRRO
- “Pathotyping” Multiplex PCR Assay for Haemophilus parasuis: a Tool for Prediction of Virulence. Journal of Clinical Microbiology, 55(9), 2617-2628. View this article in WRRO
- Sequencing a piece of history: complete genome sequence of the original Escherichia coli strain. Microbial Genomics, 3. View this article in WRRO
- Whole Genome Sequencing for Surveillance of Antimicrobial Resistance in Actinobacillus pleuropneumoniae. Frontiers in Microbiology, 8. View this article in WRRO
- Draft Genome Sequences of Three Clinical Isolates of Tannerella forsythia Isolated from Subgingival Plaque from Periodontitis Patients in the United States: TABLE 1. Genome Announcements, 4(6). View this article in WRRO
- Actinobacillus pleuropneumoniae serovar 8 predominates in England and Wales: TABLE 1:. Veterinary Record, 179, 276-276. View this article in WRRO
- ICEApl1, an integrative conjugative element related to ICEHin1056, identified in the pig pathogen Actinobacillus pleuropneumoniae. Frontiers in Microbiology, 7:810. View this article in WRRO
- Two Unrelated 8-Vinyl Reductases Ensure Production of Mature Chlorophylls in Acaryochloris marina. Journal of Bacteriology, 198(9), 1393-1400. View this article in WRRO
- A barrier to homologous recombination between sympatric strains of the cooperative soil bacterium Myxococcus xanthus. The ISME Journal, 10, 2468-2477. View this article in WRRO
- Genes Required for the Fitness of Salmonella enterica Serovar Typhimurium during Infection of Immunodeficient gp91 −/− phox Mice. Infection and Immunity, 84(4), 989-997. View this article in WRRO
- Complete Genome Sequence of MIDG2331, a Genetically Tractable Serovar 8 Clinical Isolate of Actinobacillus pleuropneumoniae. Genome Announcements, 4(1), e01667-15-e01667-15. View this article in WRRO
- Supramolecular structure in the membrane of Staphylococcus aureus. Proceedings of the National Academy of Sciences, 112(51), 15725-15730.
- Development of a Multiplex PCR Assay for Rapid Molecular Serotyping of Haemophilus parasuis. Journal of Clinical Microbiology, 53(12), 3812-3821.
- Genomic signatures of human and animal disease in the zoonotic pathogen Streptococcus suis. Nature Communications, 6(1). View this article in WRRO
- Characterisation of a mobilisable plasmid conferring florfenicol and chloramphenicol resistance in Actinobacillus pleuropneumoniae. Veterinary Microbiology, 178(3-4), 279-282.
- Erratum: Genomic signatures of human and animal disease in the zoonotic pathogen Streptococcus suis. Nature Communications, 6(1).
- Identification ofdfrA14in two distinct plasmids conferring trimethoprim resistance inActinobacillus pleuropneumoniae. Journal of Antimicrobial Chemotherapy, 70(8), 2217-2222.
- Genome-Wide DNA Methylation Patterns in Wild Samples of Two Morphotypes of Threespine Stickleback (Gasterosteus aculeatus). Molecular Biology and Evolution, 32(4), 888-895.
- Corrigendum to ‘Characterisation of a mobilisable plasmid conferring florfenicol and chloramphenicol resistance in Actinobacillus pleuropneumoniae’ [Veterinary Microbiology 178 (2015) 279–282]. Veterinary Microbiology, 179(3-4), 341-341.
- Phylogenomics of the killer whale indicates ecotype divergence in sympatry. Heredity, 114(1), 48-55.
- The use of genome wide association methods to investigate pathogenicity, population structure and serovar in Haemophilus parasuis. BMC Genomics, 15(1), 1179-1179. View this article in WRRO
- Population genomics of the killer whale indicates ecotype evolution in sympatry involving both selection and drift. Molecular Ecology, 23(21), 5179-5192.
- Multiplex PCR Assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10, and 12. Journal of Clinical Microbiology, 52(7), 2380-2385.
- Activity of Bdellovibrio Hit Locus Proteins, Bd0108 and Bd0109, Links Type IVa Pilus Extrusion/Retraction Status to Prey-Independent Growth Signalling. PLOS ONE, 8(11). View this article in WRRO
- Generation of a Tn5 transposon library in Haemophilus parasuis and analysis by transposon-directed insertion-site sequencing (TraDIS).. Vet Microbiol, 166(3-4), 558-566.
- Gene content and diversity of the loci encoding biosynthesis of capsular polysaccharides of the 15 serovar reference strains of Haemophilus parasuis.. J Bacteriol, 195(18), 4264-4273.
- Comprehensive assignment of roles for Salmonella typhimurium genes in intestinal colonization of food-producing animals.. PLoS Genet, 9(4), e1003456. View this article in WRRO
- Sequencing and functional annotation of avian pathogenic escherichia coli serogroup O78 strains reveal the evolution of E. coli lineages pathogenic for poultry via distinct mechanisms. Infection and Immunity, 81(3), 838-849.
- Transposon mutagenesis in Mycoplasma hyopneumoniae using a novel mariner-based system for generating random mutations. Veterinary Research, 44(1), 124-124.
- Development of a self-replicating plasmid system for Mycoplasma hyopneumoniae. Veterinary Research, 44(1), 63-63.
- The evolution of the Escherichia coli phylogeny.. Infect Genet Evol, 12(2), 214-226.
- Quantitative RNA-seq analysis of the Campylobacter jejuni transcriptome.. Microbiology, 157(Pt 10), 2922-2932.
- Complete genome sequence of the crohn's disease-associated adherent-invasive Escherichia coli strain HM605. Journal of Bacteriology, 193(17), 4540-4540.
- Retrospective application of transposon-directed insertion site sequencing to a library of signature-tagged mini-Tn5Km2 mutants of Escherichia coli O157:H7 screened in cattle. Journal of Bacteriology, 193(7), 1771-1776.
- Structure and function of BamE within the outer membrane and the β-barrel assembly machine.. EMBO Rep, 12(2), 123-128.
- A commensal gone bad: complete genome sequence of the prototypical enterotoxigenic Escherichia coli strain H10407.. J Bacteriol, 192(21), 5822-5831.
- Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042.. PLoS One, 5(1), e8801.
- Characterization of ISBth4, a functional new IS231 variant from Bacillus thuringiensis MEX312.. Plasmid, 63(1), 46-52.
- MycoDB: an online database for comparative genomics of the mycobacteria and related organisms.. Methods Mol Biol, 465, 419-431.
- Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).. BMC Genomics, 10, 291. View this article in WRRO
- Comprehensive identification of Salmonella enterica serovar typhimurium genes required for infection of BALB/c mice. PLoS Pathogens, 5(7). View this article in WRRO
- The pangenome structure of Escherichia coli: Comparative genomic analysis of E. coli commensal and pathogenic isolates. Journal of Bacteriology, 190(20), 6881-6893.
- xBASE2: a comprehensive resource for comparative bacterial genomics.. Nucleic Acids Res, 36(Database issue), D543-D546.
- Genome sequencing shows that European isolates of Francisella tularensis subspecies tularensis are almost identical to US laboratory strain Schu S4.. PLoS One, 2(4), e352.
- Phylogenetic comparisons reveal multiple acquisitions of the toxin genes by enterotoxigenic Escherichia coli strains of different evolutionary lineages.. J Clin Microbiol, 44(12), 4528-4536.
- Down-regulation of key virulence factors makes the Salmonella enterica serovar typhimurium rfaH mutant a promising live-attenuated vaccine candidate. Infection and Immunity, 74(10), 5914-5925.
- xBASE, a collection of online databases for bacterial comparative genomics.. Nucleic Acids Res, 34(Database issue), D335-D337.
- Escherichia coli K-12: A cooperatively developed annotation snapshot - 2005. Nucleic Acids Research, 34(1), 1-9.
- A novel strategy for the identification of genomic islands by comparative analysis of the contents and contexts of tRNA sites in closely related bacteria. Nucleic Acids Research, 34(1).
- A degenerate type III secretion system from septicemic Escherichia coli contributes to pathogenesis. Journal of Bacteriology, 187(23), 8164-8171.
- Genomic analysis of the protein secretion systems in Clostridium acetobutylicum ATCC 824.. Biochim Biophys Acta, 1745(2), 223-253.
- Prevalence of pathogenicity island IICFT073 genes among extraintestinal clinical isolates of Escherichia coli.. J Clin Microbiol, 43(5), 2425-2434.
- Bacterial flagellar diversity in the post-genomic era.. Trends Microbiol, 13(4), 143-149.
- ArrayOme: a program for estimating the sizes of microarray-visualized bacterial genomes. NUCLEIC ACIDS RESEARCH, 33(1).
- Regulators encoded in the Escherichia coli type III secretion system 2 gene cluster influence expression of genes within the locus for enterocyte effacement in enterohemorrhagic E. coli O157:H7.. Infect Immun, 72(12), 7282-7293.
- An analysis of type-III secretion gene clusters in Chromobacterium violaceum.. Trends Microbiol, 12(11), 476-482.
- The ETT2 gene cluster, encoding a second type III secretion system from Escherichia coli, is present in the majority of strains but has undergone widespread mutational attrition.. J Bacteriol, 186(11), 3547-3560.
- coliBASE: An online database for Escherichia coli, Shigella and Salmonella comparative genomics. Nucleic Acids Research, 32(DATABASE ISS.).
- coliBASE: an online database for Escherichia coli, Shigella and Salmonella comparative genomics.. Nucleic Acids Res, 32(Database issue), D296-D299.
- Genomic analysis of secretion systems.. Curr Opin Microbiol, 6(5), 519-527.
- Bacterial FHA domains: neglected players in the phospho-threonine signalling game?. Trends Microbiol, 10(12), 556-563.
- The origins of acquired immune deficiency syndrome viruses: Where and when?. Philosophical Transactions of the Royal Society B: Biological Sciences, 356(1410), 867-876.
- Genome‐enabled discovery of candidate virulence loci in Striga hermonthica , a devastating parasite of African cereal crops. New Phytologist.
- Transcriptome and proteome analysis of Salmonella enterica serovar Typhimurium systemic infection of wild type and immune-deficient mice. PLOS ONE, 12(8), e0181365-e0181365. View this article in WRRO
- Single-Step Selection of Bivalent Aptamers Validated by Comparison with SELEX Using High-Throughput Sequencing. PLoS ONE, 9(6), e100572-e100572. View this article in WRRO
- Genome-Wide High-Throughput Screening to Investigate Essential Genes Involved in Methicillin-Resistant Staphylococcus aureus Sequence Type 398 Survival. PLoS ONE, 9(2), e89018-e89018. View this article in WRRO
- The fitness landscape of the African Salmonella Typhimurium ST313 strain D23580 reveals unique properties of the pBT1 plasmid.
- Whole genome investigation of a divergent clade of the pathogen Streptococcus suis. Frontiers in Microbiology, 6. View this article in WRRO
- Corrigendum to: Probabilistic identification of bacterial essential genes via insertion density using TraDIS data with Tn5 libraries. Bioinformatics.
Chapters
- The Evolution of Primate Lentiviruses and the Origins of AIDS, The Molecular Epidemiology of Human Viruses (pp. 65-96). Springer US
- Understanding the Model and the Menace: a Postgenomic View of Escherichia Coli, Bacterial Pathogenomics (pp. 21-48). ASM Press
Preprints
- Molecular characterisation ofStreptococcus pyogenes(StrepA) non-invasive isolates during the 2022-23 UK upsurge, Cold Spring Harbor Laboratory.
- Multiple evolutionary pathways lead to vancomycin resistance in Clostridioides difficile.
- An intact S-layer is advantageous toClostridioides difficilewithin the host, Cold Spring Harbor Laboratory.
- Comparative genomic analysis of skin and soft tissue Streptococcus pyogenes isolates from low- and high-income settings, Cold Spring Harbor Laboratory.
- A Genomic Census of the Chicken Gut Microbiome using Metagenomics and Culture, Research Square Platform LLC.
- A Genomic Census of the Chicken Gut Microbiome using Metagenomics and Culture, Research Square Platform LLC.
- A Genomic Blueprint of the Chicken Gut Microbiome, Research Square Platform LLC.
- Architecture and self-assembly of theClostridium sporogenes/botulinumspore surface illustrate a general protective strategy across spore formers, Cold Spring Harbor Laboratory.
- Rationally designedmarinervectors to allow functional genomic analysis ofActinobacillus pleuropneumoniaeand other bacteria by transposon-directed insertion-site sequencing (TraDIS), Cold Spring Harbor Laboratory.
- Adding function to the genome of AfricanSalmonellaST313, Cold Spring Harbor Laboratory.
- Teaching activities
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Module Coordinator: MBB344 Genomic Science
Level 3 Modules
MBB339 Evolutionary Genetics
MBB344 Genomic Science
MBB360 Project
MBB363 Genetics Data HandlingLevel 2 Modules
MBB267 Genes, Genomes and Chromosomes
Level 1 Modules
MBB165 Practical Molecular Bioscience 1