Dr
Roy
Chaudhuri
School of Biosciences
Lecturer in Bioinformatics
r.chaudhuri@sheffield.ac.uk
+44 114 222 2837
+44 114 222 2837
Firth Court
Full contact details
Dr Roy Chaudhuri
School of Biosciences
Firth Court
School of Biosciences
Firth Court
Western Bank
Sheffield
S10 2TN
- Research interests
-
My research interests are primarily in the area of bacterial genomics.
Current research topics include:
- Comparative genomics and phylogenetics of bacterial pathogens, particularly E. coli and Salmonella
- Use of transposon insertion sequencing methods (TraDIS/TnSeq/HITS/InSeq) to identify essential bacterial genes and genes important for survival in particular environments such as during infection of a model system
- Development of user-friendly software tools and online resources for exploring data from -omics technologies. Examples include coliBASE, Xbase and the recently-funded MicrobesNG.
- Publications
-
Show: Featured publications All publications
Featured publications
Journal articles
- Genes Required for the Fitness of Salmonella enterica Serovar Typhimurium during Infection of Immunodeficient gp91 −/− phox Mice. Infection and Immunity, 84(4), 989-997.
- The use of genome wide association methods to investigate pathogenicity, population structure and serovar in Haemophilus parasuis. BMC Genomics, 15. View this article in WRRO
- Generation of a Tn5 transposon library in Haemophilus parasuis and analysis by transposon-directed insertion-site sequencing (TraDIS).. Vet Microbiol, 166(3-4), 558-566.
- Gene content and diversity of the loci encoding biosynthesis of capsular polysaccharides of the 15 serovar reference strains of Haemophilus parasuis.. J Bacteriol, 195(18), 4264-4273.
- Comprehensive assignment of roles for Salmonella typhimurium genes in intestinal colonization of food-producing animals.. PLoS Genet, 9(4), e1003456.
- The evolution of the Escherichia coli phylogeny.. Infect Genet Evol, 12(2), 214-226.
- Quantitative RNA-seq analysis of the Campylobacter jejuni transcriptome.. Microbiology (Reading), 157(Pt 10), 2922-2932.
- Retrospective application of transposon-directed insertion site sequencing to a library of signature-tagged mini-Tn5Km2 mutants of Escherichia coli O157:H7 screened in cattle. Journal of Bacteriology, 193(7), 1771-1776.
- A commensal gone bad: complete genome sequence of the prototypical enterotoxigenic Escherichia coli strain H10407.. J Bacteriol, 192(21), 5822-5831.
- Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042.. PLoS One, 5(1), e8801.
- Comprehensive identification of Salmonella enterica serovar typhimurium genes required for infection of BALB/c mice. Plos Pathogens, 5(7).
- Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).. BMC Genomics, 10, 291.
- The pangenome structure of Escherichia coli: Comparative genomic analysis of E. coli commensal and pathogenic isolates. Journal of Bacteriology, 190(20), 6881-6893.
- Escherichia coli K-12: A cooperatively developed annotation snapshot - 2005. Nucleic Acids Research, 34(1), 1-9.
- xBASE, a collection of online databases for bacterial comparative genomics.. Nucleic Acids Res, 34(Database issue), D335-D337.
- Genomic signatures of human and animal disease in the zoonotic pathogen Streptococcus suis. Nature Communications, 6. View this article in WRRO
All publications
Journal articles
- Molecular analysis of Lancefield group C/G streptococci causing human infections in Sheffield, UK. Microbial Genomics, 12(5).
- A set of genetic tools for use in Clostridioides difficile and related species. Microbiology, 172(2).
- Identification of pathways to high-level vancomycin resistance in Clostridioides difficile that incur high fitness costs in key pathogenicity traits. PLOS Biology, 22(8). View this article in WRRO
- Molecular characterization of Streptococcus pyogenes (StrepA) non-invasive isolates during the 2022–2023 UK upsurge. Microbial Genomics, 10(8). View this article in WRRO
- DUF916 and DUF3324 in the WxL protein cluster bind to WxL and link bacterial and host surfaces. Protein Science, 32(11). View this article in WRRO
- An intact S-layer is advantageous to Clostridioides difficile within the host. PLOS Pathogens, 19(6). View this article in WRRO
- Genomic characterization of skin and soft tissue Streptococcus pyogenes isolates from a low-income and a high-income setting. mSphere, 8(1). View this article in WRRO
- Genome-enabled discovery of candidate virulence loci in Striga hermonthica, a devastating parasite of African cereal crops. New Phytologist, 236(2), 622-638. View this article in WRRO
- Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site. Microbiology, 167(12). View this article in WRRO
- Rationally designed mariner vectors for functional genomic analysis of Actinobacillus pleuropneumoniae and other Pasteurellaceae species by transposon-directed insertion-site sequencing (TraDIS). Animal Diseases, 1(1).
- Corrigendum to: Probabilistic identification of bacterial essential genes via insertion density using TraDIS data with Tn5 libraries. Bioinformatics, 37(19), 3387-3387.
- Probabilistic identification of bacterial essential genes via insertion density using TraDIS data with Tn5 libraries. Bioinformatics, 37(23), 4343-4349. View this article in WRRO
- Extensive microbial diversity within the chicken gut microbiome revealed by metagenomics and culture. PeerJ, 9.
- Architecture and self-assembly of clostridium sporogenes and clostridium botulinum spore surfaces illustrate a general protective strategy across spore formers. mSphere, 5(4). View this article in WRRO
- Increasing prevalence of a fluoroquinolone resistance mutation amongst Campylobacter jejuni isolates from four human infectious intestinal disease studies in the United Kingdom. PLoS ONE, 15(1).
- Draft Genome Sequences of the Type Strains of Actinobacillus indolicus (46K2C) and Actinobacillus porcinus (NM319), Two NAD-Dependent Bacterial Species Found in the Respiratory Tract of Pigs. Microbiology Resource Announcements, 9(1).
- Publisher Correction: Genomic signatures of human and animal disease in the zoonotic pathogen Streptococcus suis. Nature Communications, 10(1).
- The fitness landscape of the African salmonella typhimurium ST313 strain D23580 reveals unique properties of the pBT1 plasmid. PLOS Pathogens, 15(9).
- Draft whole-genome sequences of 10 Aeromonas strains from clinical and environmental sources. Microbiology Resource Announcements, 8(30). View this article in WRRO
- The fitness landscape of the AfricanSalmonellaTyphimurium ST313 strain D23580 reveals unique properties of the pBT1 plasmid.
- Proteomic profiling, transcription factor modeling, and genomics of evolved tolerant strains elucidate mechanisms of vanillin toxicity in Escherichia coli.. mSystems, 4(4). View this article in WRRO
- Applying transposon-directed insertion site sequencing to industrially important, solventogenic species Clostridium saccharoperbutylacetonicum. Access Microbiology, 1(1A).
- Adding function to the genome of African Salmonella Typhimurium ST313 strain D23580.. PLoS Biology, 17(1). View this article in WRRO
- Retrospective application of transposon-directed insertion-site sequencing to investigate niche-specific virulence of Salmonella Typhimurium in cattle.. BMC Genomics, 20. View this article in WRRO
- Use of Proteins Identified through a Functional Genomic Screen To Develop a Protein Subunit Vaccine That Provides Significant Protection against Virulent Streptococcus suis in Pigs. Infection and Immunity, 86(3). View this article in WRRO
- "Pathotyping" Multiplex PCR Assay for Haemophilus parasuis: a Tool for Prediction of Virulence. Journal of Clinical Microbiology, 55(9), 2617-2628. View this article in WRRO
- Transcriptome and proteome analysis of Salmonella enterica serovar Typhimurium systemic infection of wild type and immune-deficient mice. PLoS ONE, 12(8). View this article in WRRO
- Sequencing a piece of history: complete genome sequence of the original Escherichia coli strain. Microbial Genomics, 3. View this article in WRRO
- Whole Genome Sequencing for Surveillance of Antimicrobial Resistance in Actinobacillus pleuropneumoniae. Frontiers in Microbiology, 8. View this article in WRRO
- Draft Genome Sequences of Three Clinical Isolates of Tannerella forsythia Isolated from Subgingival Plaque from Periodontitis Patients in the United States.. Genome Announcements, 4(6). View this article in WRRO
- Actinobacillus pleuropneumoniae serovar 8 predominatesin England and Wales. Veterinary Record, 179(11). View this article in WRRO
- ICEApl1, an integrative conjugative element related to ICEHin1056, identified in the pig pathogen Actinobacillus pleuropneumoniae. Frontiers in Microbiology, 7:810.
- Two Unrelated 8-Vinyl Reductases Ensure Production of Mature Chlorophylls in Acaryochloris marina. JOURNAL OF BACTERIOLOGY, 198(9), 1393-1400. View this article in WRRO
- A barrier to homologous recombination between sympatric strains of the cooperative soil bacterium Myxococcus xanthus. The ISME Journal, 10, 2468-2477.
- Genes Required for the Fitness of Salmonella enterica Serovar Typhimurium during Infection of Immunodeficient gp91 −/− phox Mice. Infection and Immunity, 84(4), 989-997.
- Complete Genome Sequence of MIDG2331, a Genetically Tractable Serovar 8 Clinical Isolate of Actinobacillus pleuropneumoniae. Genome Announcements, 4(1), e01667-15-e01667-15.
- Supramolecular structure in the membrane of Staphylococcus aureus. Proceedings of the National Academy of Sciences, 112(51), 15725-15730.
- Development of a Multiplex PCR Assay for Rapid Molecular Serotyping of Haemophilus parasuis. Journal of Clinical Microbiology, 53(12), 3812-3821.
- Whole genome investigation of a divergent clade of the pathogen Streptococcus suis. Frontiers in Microbiology , 6. View this article in WRRO
- Corrigendum to ‘Characterisation of a mobilisable plasmid conferring florfenicol and chloramphenicol resistance in Actinobacillus pleuropneumoniae’ [Veterinary Microbiology 178 (2015) 279–282]. Veterinary Microbiology, 179(3-4), 341-341.
- Characterisation of a mobilisable plasmid conferring florfenicol and chloramphenicol resistance in Actinobacillus pleuropneumoniae. Veterinary Microbiology, 178(3-4), 279-282.
- Identification of dfrA14 in two distinct plasmids conferring trimethoprim resistance in Actinobacillus pleuropneumoniae. Journal of Antimicrobial Chemotherapy, 70(8), 2217-2222.
- Genome-Wide DNA Methylation Patterns in Wild Samples of Two Morphotypes of Threespine Stickleback (Gasterosteus aculeatus). Molecular Biology and Evolution, 32(4), 888-895.
- Phylogenomics of the killer whale indicates ecotype divergence in sympatry. Heredity, 114(1), 48-55.
- The use of genome wide association methods to investigate pathogenicity, population structure and serovar in Haemophilus parasuis. BMC Genomics, 15. View this article in WRRO
- Population genomics of the killer whale indicates ecotype evolution in sympatry involving both selection and drift. Molecular Ecology, 23(21), 5179-5192.
- Multiplex PCR Assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10, and 12. Journal of Clinical Microbiology, 52(7), 2380-2385.
- Single-Step Selection of Bivalent Aptamers Validated by Comparison with SELEX Using High-Throughput Sequencing. PLoS ONE, 9(6). View this article in WRRO
- Genome-Wide High-Throughput Screening to Investigate Essential Genes Involved in Methicillin-Resistant Staphylococcus aureus Sequence Type 398 Survival. PLoS ONE, 9(2). View this article in WRRO
- Transposon mutagenesis in Mycoplasma hyopneumoniae using a novel mariner-based system for generating random mutations. Veterinary Research, 44(1).
- Activity of Bdellovibrio Hit Locus Proteins, Bd0108 and Bd0109, Links Type IVa Pilus Extrusion/Retraction Status to Prey-Independent Growth Signalling. PLOS ONE, 8(11).
- Development of a self-replicating plasmid system for Mycoplasma hyopneumoniae. Veterinary Research, 44(1).
- Generation of a Tn5 transposon library in Haemophilus parasuis and analysis by transposon-directed insertion-site sequencing (TraDIS).. Vet Microbiol, 166(3-4), 558-566.
- Gene content and diversity of the loci encoding biosynthesis of capsular polysaccharides of the 15 serovar reference strains of Haemophilus parasuis.. J Bacteriol, 195(18), 4264-4273.
- Comprehensive assignment of roles for Salmonella typhimurium genes in intestinal colonization of food-producing animals.. PLoS Genet, 9(4), e1003456.
- Sequencing and functional annotation of avian pathogenic escherichia coli serogroup O78 strains reveal the evolution of E. coli lineages pathogenic for poultry via distinct mechanisms. Infection and Immunity, 81(3), 838-849.
- The evolution of the Escherichia coli phylogeny.. Infect Genet Evol, 12(2), 214-226.
- Quantitative RNA-seq analysis of the Campylobacter jejuni transcriptome.. Microbiology (Reading), 157(Pt 10), 2922-2932.
- Complete genome sequence of the crohn's disease-associated adherent-invasive Escherichia coli strain HM605. Journal of Bacteriology, 193(17), 4540-4540.
- Retrospective application of transposon-directed insertion site sequencing to a library of signature-tagged mini-Tn5Km2 mutants of Escherichia coli O157:H7 screened in cattle. Journal of Bacteriology, 193(7), 1771-1776.
- Structure and function of BamE within the outer membrane and the β-barrel assembly machine.. EMBO Rep, 12(2), 123-128.
- A commensal gone bad: complete genome sequence of the prototypical enterotoxigenic Escherichia coli strain H10407.. J Bacteriol, 192(21), 5822-5831.
- Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042.. PLoS One, 5(1), e8801.
- Characterization of ISBth4, a functional new IS231 variant from Bacillus thuringiensis MEX312.. Plasmid, 63(1), 46-52.
- MycoDB: an online database for comparative genomics of the mycobacteria and related organisms.. Methods Mol Biol, 465, 419-431.
- Comprehensive identification of Salmonella enterica serovar typhimurium genes required for infection of BALB/c mice. Plos Pathogens, 5(7).
- Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).. BMC Genomics, 10, 291.
- The pangenome structure of Escherichia coli: Comparative genomic analysis of E. coli commensal and pathogenic isolates. Journal of Bacteriology, 190(20), 6881-6893.
- xBASE2: a comprehensive resource for comparative bacterial genomics.. Nucleic Acids Res, 36(Database issue), D543-D546.
- Genome sequencing shows that European isolates of Francisella tularensis subspecies tularensis are almost identical to US laboratory strain Schu S4.. PLoS One, 2(4), e352.
- Phylogenetic comparisons reveal multiple acquisitions of the toxin genes by enterotoxigenic Escherichia coli strains of different evolutionary lineages.. J Clin Microbiol, 44(12), 4528-4536.
- Down-regulation of key virulence factors makes the Salmonella enterica serovar typhimurium rfaH mutant a promising live-attenuated vaccine candidate. Infection and Immunity, 74(10), 5914-5925.
- A novel strategy for the identification of genomic islands by comparative analysis of the contents and contexts of tRNA sites in closely related bacteria. Nucleic Acids Research, 34(1), e3.
- Escherichia coli K-12: A cooperatively developed annotation snapshot - 2005. Nucleic Acids Research, 34(1), 1-9.
- xBASE, a collection of online databases for bacterial comparative genomics.. Nucleic Acids Res, 34(Database issue), D335-D337.
- A degenerate type III secretion system from septicemic Escherichia coli contributes to pathogenesis. Journal of Bacteriology, 187(23), 8164-8171.
- Genomic analysis of the protein secretion systems in Clostridium acetobutylicum ATCC 824.. Biochim Biophys Acta, 1745(2), 223-253.
- Prevalence of pathogenicity island IICFT073 genes among extraintestinal clinical isolates of Escherichia coli.. J Clin Microbiol, 43(5), 2425-2434.
- ArrayOme: a program for estimating the sizes of microarray-visualized bacterial genomes. NUCLEIC ACIDS RESEARCH, 33(1).
- Bacterial flagellar diversity in the post-genomic era.. Trends Microbiol, 13(4), 143-149.
- Regulators encoded in the Escherichia coli type III secretion system 2 gene cluster influence expression of genes within the locus for enterocyte effacement in enterohemorrhagic E. coli O157:H7.. Infect Immun, 72(12), 7282-7293.
- The ETT2 gene cluster, encoding a second type III secretion system from Escherichia coli, is present in the majority of strains but has undergone widespread mutational attrition.. J Bacteriol, 186(11), 3547-3560.
- coliBASE: An online database for Escherichia coli, Shigella and Salmonella comparative genomics. Nucleic Acids Research, 32(DATABASE ISS.), D296-D299.
- An analysis of type-III secretion gene clusters in Chromobacterium violaceum.. Trends Microbiol, 12(11), 476-482.
- coliBASE: an online database for Escherichia coli, Shigella and Salmonella comparative genomics.. Nucleic Acids Res, 32(Database issue), D296-D299.
- Genomic analysis of secretion systems.. Curr Opin Microbiol, 6(5), 519-527.
- Bacterial FHA domains: neglected players in the phospho-threonine signalling game?. Trends Microbiol, 10(12), 556-563.
- The origins of acquired immune deficiency syndrome viruses: Where and when?. Philosophical Transactions of the Royal Society B Biological Sciences, 356(1410), 867-876.
- Genomic signatures of human and animal disease in the zoonotic pathogen Streptococcus suis. Nature Communications, 6. View this article in WRRO
- Erratum: Genomic signatures of human and animal disease in the zoonotic pathogen Streptococcus suis. Nature Communications, 6(1).
Book chapters
- The Evolution of Primate Lentiviruses and the Origins of AIDS, The Molecular Epidemiology of Human Viruses (pp. 65-96). Springer US
- Understanding the Model and the Menace: a Postgenomic View ofEscherichia Coli, Bacterial Pathogenomics (pp. 21-48). ASM Press
Conference proceedings
- Validation Study of the Parkinson's disease (PD) Stigma Questionnaire (PDStigmaQuest). MOVEMENT DISORDERS, Vol. 39 (pp S239-S239)
- Pain in People with Multiple System Atrophy. MOVEMENT DISORDERS, Vol. 38 (pp S65-S66)
- Safety and tolerability of apomorphine sublingual film and subcutaneous apomorphine for the treatment of OFF episodes in Parkinson's disease. MOVEMENT DISORDERS, Vol. 37 (pp S348-S348)
- Efficacy of apomorphine sublingual film versus subcutaneous apomorphine for the treatment of OFF episodes in Parkinson's Disease. MOVEMENT DISORDERS, Vol. 37 (pp S356-S356)
- Challenges in Outcome Measure Selection in Multi-Arm Multi-Stage Clinical Trials in Parkinson Disease. MOVEMENT DISORDERS, Vol. 37 (pp S329-S329)
- Type 2 diabetes mellitus may worsen severity of motor symptoms in people with Parkinson's disease. MOVEMENT DISORDERS, Vol. 35 (pp S153-S153)
Preprints
- Molecular analysis of Lancefield group C/G streptococci causing human infections in Sheffield, UK, openRxiv.
- Molecular characterisation ofStreptococcus pyogenes(StrepA) non-invasive isolates during the 2022-23 UK upsurge, Cold Spring Harbor Laboratory.
- Comparative genomic analysis of skin and soft tissue Streptococcus pyogenes isolates from low- and high-income settings, Cold Spring Harbor Laboratory.
- A Genomic Census of the Chicken Gut Microbiome using Metagenomics and Culture, Springer Science and Business Media LLC.
- A Genomic Census of the Chicken Gut Microbiome using Metagenomics and Culture, Springer Science and Business Media LLC.
- A Genomic Blueprint of the Chicken Gut Microbiome, Springer Science and Business Media LLC.
- Architecture and self-assembly of theClostridium sporogenes/botulinumspore surface illustrate a general protective strategy across spore formers, Cold Spring Harbor Laboratory.
- Adding function to the genome of AfricanSalmonellaST313, Cold Spring Harbor Laboratory.
- Rationally designedmarinervectors to allow functional genomic analysis ofActinobacillus pleuropneumoniaeand other bacteria by transposon-directed insertion-site sequencing (TraDIS), Cold Spring Harbor Laboratory.
- Genes Required for the Fitness of Salmonella enterica Serovar Typhimurium during Infection of Immunodeficient gp91 −/− phox Mice. Infection and Immunity, 84(4), 989-997.
- Teaching activities
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Module Coordinator: MBB344 Genomic Science
Level 3 Modules
MBB339 Evolutionary Genetics
MBB344 Genomic Science
MBB360 Project
MBB363 Genetics Data HandlingLevel 2 Modules
MBB267 Genes, Genomes and Chromosomes
Level 1 Modules
MBB165 Practical Molecular Bioscience 1