Dr Anne-Gaelle Borycki
School of Biosciences
Senior Lecturer
a.g.borycki@sheffield.ac.uk
D19, Firth Court
Full contact details
Dr Anne-Gaelle Borycki
School of Biosciences
D19
Firth Court
Western Bank
Sheffield
S10 2TN
School of Biosciences
D19
Firth Court
Western Bank
Sheffield
S10 2TN
- Profile
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- 2011-present: Senior Lecturer, School of Biosciences
- 2000-2010: Lecturer, Department of Biomedical Science
- 1994-2000: Post-doctoral Fellow at the University of Pennsylvania Medical School in Philadelphia - USA (Advisor: Prof: Charles P. Emerson, Jr)
- 1992-1994: Post-doctoral Fellow at Gustave Roussy Institute for Cancer Research in Villejuif - France (Advisor: Dr. Serge A. Leibovitch)
- 1992: Ph.D in Molecular Biology from University of Science, Paris - France (Supervisor: Dr. Serge A. Leibovitch)
- 1987: M.Sc in Biochemistry from University of Science, Paris - France
- 1986: B.Sc. in Biochemistry from University of Science, Paris - France
- Research interests
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The Borycki lab uses primarily mouse genetics to study how the extra-cellular matrix, Sonic hedgehog signaling and cellular structures such as the primary cilum contribute to the regulation of satellite cell activity and to skeletal muscle regeneration.
Satellite cell biology and skeletal muscle regeneration
Satellite cells are skeletal muscle-specific stem cells that reside in a sub-laminal position at the periphery of myofibres. Satellite cells are normally quiescent, but become activated and expand rapidly to produce new muscle progenitor cells that differentiate and fuse to damaged fibres to repair diseased or injured muscles. Therefore, satellite cells hold the promise to be harnessed for the treatment of muscular dystrophies or age-related sarcopenia. However, the use of satellite cells for therapeutic purpose requires prior knowledge of their biology.
Research in our lab focuses on several aspects of satellite cell biology:
- The satellite cell niche, and in particular the role played by the extra-cellular matrix and its receptors in the control of adult myogenesis. We have recently demonstrated a remodelling of the extra-cellular matrix at the satellite cell niche during muscle regeneration. Current research aims at translating these findings to develop better in vitro conditions for the expansion of satellite cells.
- The signalling events that regulate satellite cell behaviour; We have uncovered a role for Sonic hedgehog signalling in the control of satellite cell progression through the cell cycle during muscle regeneration. Future work will focus on the implications of these findings in muscle tumours.
- The requirement for primary cilia for cell signalling and for mechanotransduction in injured muscles.
To investigate these issues, we use a combination of ex-vivo and in-vivo approaches in the mouse, as well as mouse genetic tools such as conditional knockout, transgenic reporter, and gain-of-function mutant lines.
Our current external collaborations include:
Peter Zammit (UCL), Jennifer Morgan (UCL), Paolo de Coppi (UCL), James Briscoe (Crick Institute), Olivier Lefebvre (Strasbourg).
- Publications
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Show: Featured publications All publications
Featured publications
Journal articles
- Basal lamina remodeling at the skeletal muscle stem cell niche mediates stem cell self-renewal. Nature Communications, 9. View this article in WRRO
- Hedgehog signalling acts upstream of Laminin alpha1 transcription in the zebrafish paraxial mesoderm. Matrix Biology, 62, 58-74. View this article in WRRO
- Asymmetric Distribution of Primary Cilia Allocates Satellite Cells for Self-Renewal. Stem Cell Reports, 6(6), 788-805. View this article in WRRO
- The myotomal basement membrane: Insight into laminin-111 function and its control by Sonic hedgehog signaling. Cell Adhesion and Migration, 7(1), 72-81.
- Sonic hedgehog acts cell-autonomously on muscle precursor cells to generate limb muscle diversity. Genes and Development, 26(18), 2103-2117.
- Sonic hedgehog-dependent synthesis of laminin α1 controls basement membrane assembly in the myotome. Development, 136(20), 3495-3504.
All publications
Journal articles
- Basal lamina remodeling at the skeletal muscle stem cell niche mediates stem cell self-renewal. Nature Communications, 9. View this article in WRRO
- Hedgehog signalling acts upstream of Laminin alpha1 transcription in the zebrafish paraxial mesoderm. Matrix Biology, 62, 58-74. View this article in WRRO
- Asymmetric Distribution of Primary Cilia Allocates Satellite Cells for Self-Renewal. Stem Cell Reports, 6(6), 788-805. View this article in WRRO
- The myotomal basement membrane: Insight into laminin-111 function and its control by Sonic hedgehog signaling. Cell Adhesion and Migration, 7(1), 72-81.
- Sonic hedgehog acts cell-autonomously on muscle precursor cells to generate limb muscle diversity. Genes and Development, 26(18), 2103-2117.
- Distinct spatiotemporal roles of hedgehog signalling during chick and mouse cranial base and axial skeleton development. Developmental Biology.
- FatJ acts via the Hippo mediator Yap1 to restrict the size of neural progenitor cell pools.. Development, 138(10), 1893-1902.
- A novel Gli3 enhancer controls the Gli3 spatiotemporal expression pattern through a TALE homeodomain protein binding site. Molecular and Cellular Biology, 31(7), 1432-1443.
- Expression analysis of TALE family transcription factors during avian development. Developmental Dynamics, 239(4), 1234-1245.
- Sonic hedgehog-dependent synthesis of laminin α1 controls basement membrane assembly in the myotome. Development, 136(20), 3495-3504.
- Dystroglycan protein distribution coincides with basement membranes and muscle differentiation during mouse embryogenesis.. Dev Dyn, 236(9), 2627-2635.
- Expression of avian C-terminal binding proteins (Ctbp1 and Ctbp2) during embryonic development. Developmental Dynamics, 235(2), 490-495.
- Gli2 and Gli3 have redundant and context-dependent function in skeletal muscle formation. Development, 132(2), 345-357.
- Expression of avian Groucho-related genes (Grgs) during embryonic development. Gene Expression Patterns, 5(6), 817-823.
- Shh and Wnt signaling pathways converge to control Gli gene activation in avian somites.. Development, 127(10), 2075-2087.
- Study of skeletal myogenesis in cultures of unsegmented paraxial mesoderm.. Methods in molecular biology (Clifton, N.J.), 137, 351-357.
- 5 Multiple Tissue Interactions and Signal Transduction Pathways Control Somite Myogenesis. Current Topics in Developmental Biology, 48(C), 165-224.
- Pax3 functions in cell survival and in pax7 regulation. Development, 126(8), 1665-1674.
- Sonic hedgehog controls epaxial muscle determination through Myf5 activation. Development, 126(18), 4053-4063.
- Differentiation of avian craniofacial muscles: I. Patterns of early regulatory gene expression and myosin heavy chain synthesis. Developmental Dynamics, 216(2), 96-112.
- Control of somite patterning by Sonic hedgehog and its downstream signal response genes. Development, 125(4), 777-790.
- Distinct signal/response mechanisms regulate pax1 and QmyoD activation in sclerotomal and myotomal lineages of quail somites. Developmental Biology, 185(2), 185-200.
- Muscle determination: Another key player in myogenesis?. Current Biology, 7(10).
- Repression of the CSF-1 receptor (c-fms proto-oncogene product) by antisense transfection induces G1-growth arrest in L6α1 rat myoblasts. Oncogene, 10(9), 1799-1811.
- Colony-Stimulating Factor 1 (CSF-1)Is Involved in an Autocrine Growth Control of Rat Myogenic Cells. Experimental Cell Research, 218(1), 213-222.
- Isolation and characterization of a cDNA clone encoding for rat CSF-1 gene. Post-transcriptional repression occurs in myogenic differentiation.. Biochim Biophys Acta, 1174(2), 143-152.
- Molecular Cloning of CSF-1 Receptor from Rat Myoblasts. Sequence Analysis and Regulation During Myogenesis. Growth Factors, 6(4), 209-218.
- Molecular cloning of CSF-1 receptor from rat myoblasts. Sequence analysis and regulation during myogenesis. Growth Factors, 6(3), 209-218.
- Proto‐oncogenes and Differentiation versus Transformation of Striated Muscle Cells. Annals of the New York Academy of Sciences, 567(1), 187-207.
- Expression of CSF-1-receptor-related proteins in muscular stem cells. Oncogene Research, 4(2), 157-162.
Chapters
- Light-Sheet Microscopy Enables Three-Dimensional Fluorescence Imaging and Live Imaging of Satellite Cells on Skeletal Muscle Fibers, Methods in Molecular Biology Springer US
- Sequence analyses to study the evolutionary history and cis-regulatory elements of hedgehog genes (pp. 231-250).
- Hedgehog Signalling John Wiley & Sons, Ltd
Conference proceedings papers
- The role of a novel Sonic hedgehog target gene in the formation of motor neurons during zebrafish embryonic development. MECHANISMS OF DEVELOPMENT, Vol. 126 (pp S315-S315)
- Sonic hedgehog-dependent control of basement membrane assembly in the myotome. MECHANISMS OF DEVELOPMENT, Vol. 126 (pp S196-S197)
Preprints
- Basal lamina remodeling at the skeletal muscle stem cell niche mediates stem cell self-renewal. Nature Communications, 9. View this article in WRRO
- Grants
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- Funding
- Wellcome Trust
- BBSRC
- MRC
- European Union
- AFM (Association Francaise contre les Myopathies)
- Teaching activities
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Undergraduate and postgraduate taught modules
Undergraduate:
- BMS109-108 Physiology with Pharmacology
- BMS110 Research Topics in Biomedicine
- BMS242/243 Principles of Developmental Biology (Co-ordinator)
- Level 3 Practical and Dissertation Modules
Postgraduate:
- BMS6053 Critical Analysis of Current Science (Co-ordinator)
- Level 4 Tutor
- Professional activities and memberships
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- Member of the EU-funded Network of Excellence "Cells into Organs"
- Member and Research Program coordinator of the EU-funded Network of Excellence "MYORES"
- Panel member since 2007, Muscle Biology Committee, AFM (Association Francaise contre les Myopathies)
- PhD Studentship Opportunities
We advertise PhD opportunities (Funded or Self-Funded) on FindAPhD.com
For further information and details of other projects on offer, please see the department PhD Opportunities page: