biOMICS Mass Spectrometry Instruments

Capabilities

We apply various proteomics and metabolomics strategies to address and answer your research questions. We also host state-of-the-art infrastructure for identification, characterisation and quantitation of small to large biological molecules using advanced mass spectrometry based approaches.

Proteomics

• Protein identification, quantification and characterisation

  • Gel-based proteomics
  • Shot-gun proteomics
  • Proteome profiling
  • Protein-protein interactions
  • Post-translational modifications
  • Biomarker discovery
  • Relative and absolute quantitation
  • Multiplexing quantitation

Metabolomics

• Metabolite identification, quantification and characterisation

  • Plant metabolism studies
  • Drug metabolism studies
  • Metabolic disorders
  • Biomarker discovery
  • Cell development stage

• Mass spec imaging

  • MALDI/DESI imaging

• Isotope ratio analysis

  • Stable carbon and nitrogen isotope ratio measurement
  • Isotopic labelling tracer studies

Instruments

Mass Spectrometry (MS)

> Bruker tims TOF fleX MALDI-2 

	MALDI-MS-imaging down to 5 µm spatial resolution UPLC-MS targeted analysis Non-targeted metabolic profiling MS/MS and TIMS analysis of ions for mass identification Rapid DDA and DIA-PASEF (ion mobility enabled) proteomic analysis

> Thermo Orbitrap Exploris 480 

Shotgun proteomics/peptide characterisation High resolution reporter ion quantification (TMT) SureQuant (internal standard triggered targeted quantification of peptides/proteins Absolute or relative quantitation through methodologies such as AQUA, SILAC, LFQ (label-free quantification), isobaric tags (TMT, iTRAQ) Post-translational modifications of proteins such as phosphorylation, acetylation, methylation and ubiquitination

> Waters G2/G2Si Synapt

	Non-targeted metabolic profiling UPLC-MS targeted analysis MS/MS and ion mobility for analysis of ions for mass identification MALDI-MS/DESI-MS- analysis and imaging

> Perkin Elmer GC-MS

	Volatile Analysis Thermal desorption GC-MS

> Sercon IRMS 

	Carbon and nitrogen isotope ratios Solids, liquids, gases

HPLC

• Agilent 11290 Infinity II
• Evosep (high throughput nanoflow chromatography)
• Nanoelute (nanoflow chromatography)
• Vanquish Neo UHPLC (nanoflow-microflow chromatography)
• Dionex U3000 BioRS (offline peptide fractionation)
• Waters Acquity iClass UPLC
• Waters Alliance HPLC
• Waters UPC2

Portable mass specs

• The Torion® T-9 GC-MS
• Advion Expression^s CMS

Sample Prep

• Beat Box (High-throughput sample homogenisation)
• Tecan A200 Positive Pressure System (solid phase extraction)
• HTX M3+ Sprayer

Contact

• General requests massspectrometry@sheffield.ac.uk
• Metabolomics h.j.walker@sheffield.ac.uk
• Proteomics khoa.pham@sheffield.ac.uk