Dr Nicola Green
PhD FRMS
School of Chemical, Materials and Biological Engineering
School of Chemical, Materials and Biological Engineering
Senior Lecturer in Bioengineering
Biomedical engineering programme lead
n.h.green@sheffield.ac.uk
+44 114 222 3932
+44 114 222 3932
North Campus
Full contact details
Dr Nicola Green
School of Chemical, Materials and Biological Engineering
North Campus
Broad Lane
Sheffield
S3 7HQ
School of Chemical, Materials and Biological Engineering
North Campus
Broad Lane
Sheffield
S3 7HQ
- Profile
-
I am a Senior Lecturer in Bioengineering. I obtained my first degree In Biochemistry and Genetics from the University of Nottingham and my PhD in Biotechnology from the University of Kent. After my PhD I trained and worked as a science teacher before returning to the academic research environment. I worked as a Research Fellow at the University of Nottingham, in the Laboratory of Biophysics and Surface Analysis, and as a Senior Scientist at Scancell, a Nottingham University biomedical spin-off company.
I joined the University of Sheffield 2006 as a postdoctoral researcher in biomaterials and tissue engineering and took charge of the Kroto Imaging Facility as Experimental Officer in 2010. I was appointed as Lecturer in Bioengineering in 2019 and Senior Lecturer in 2022.
- Qualifications
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1998 PhD – Biotechnology, University of Kent
1992 – BSc Hons Biochemistry and Genetics, Nottingham University
- Research interests
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My research aims to understand the interactions between biomaterials, cells and the environment that they are being cultured in. This allows me to use cells in the manufacturing process to create novel biomaterials containing both synthetic and biological components and leads to a better understanding of how materials will behave once they are implanted into the body to repair and replace damaged tissue. We also use this understanding to create better, more biologically relevant 3D models of specific tissues and diseases that can be used in the laboratory to improve the drug discovery pipeline and replace animal experiments.
Key research interests:
- Biohybrid scaffolds for tissue engineering: Scaffolds which incorporate biological components into synthetic scaffolds combine benefits from both and have significant clinical potential. This research seeks to optimise the composition and manufacture of these scaffolds to create tuneable scaffolds with enhanced cellular responses and reduced immunogenicity.
- Improved in vitro models: The development and use of in vitro 3D models for improved drug discovery and to more reliably model disease progression including cancer invasion and metastasis, wound healing, and the musculoskeletal system.
- Imaging of biomaterials and tissues: Development and application of non-invasive imagine techniques to better characterise biomaterials and the cellular response.
- Publications
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Journal articles
- Highly porous polycaprolactone microspheres for skeletal repair promote a mature bone cell phenotype in vitro.. J Mater Chem B.
- Inhibition and reversal of a TGF-β1 induced myofibroblast phenotype by adipose tissue-derived paracrine factors.. Stem Cell Res Ther, 15(1), 166.
- The use of microphysiological systems to model metastatic cancer. Biofabrication, 16(3). View this article in WRRO
- Surfactant-free gelatin-stabilised biodegradable polymerised high internal phase emulsions with macroporous structures. Frontiers in Chemistry, 11.
- Synthesis and characterisation of photocurable poly(glycerol sebacate)-co-poly(ethylene glycol) methacrylates. Materials Today Advances, 19, 100410-100410.
- Porous biomaterials for tissue engineering: a review. Journal of Materials Chemistry B.
- Electrospun fiber alignment guides osteogenesis and matrix organization differentially in two different osteogenic cell types. Frontiers in Bioengineering and Biotechnology, 9. View this article in WRRO
- In vitro low-fluence photodynamic therapy parameter screening using 3D tumor spheroids shows that fractionated light treatments enhance phototoxicity. ACS Biomaterials Science & Engineering. View this article in WRRO
- Identifying and mapping chemical bonding within phenolic resin using Secondary Electron Hyperspectral Imaging. Polymer Chemistry. View this article in WRRO
- Characterizing cross‐linking within polymeric biomaterials in the SEM by secondary electron hyperspectral imaging. Macromolecular Rapid Communications, 41(3). View this article in WRRO
- Two photon excitable graphene quantum dots for structured illumination microscopy and imaging applications: lysosome specificity and tissue-dependent imaging. Chemical Communications. View this article in WRRO
- Porous microspheres support mesenchymal progenitor cell ingrowth and stimulate angiogenesis. APL Bioengineering. View this article in WRRO
- Measurement of friction-induced changes in pig aorta fibre organization by non-invasive imaging as a model for detecting the tissue response to endovascular catheters. Biotribology, 12, 24-32. View this article in WRRO
- Imaging cellular trafficking processes in real time using lysosome targeted up-conversion nanoparticles.. Chemical Communications, 53, 12672-12675. View this article in WRRO
- A new mode of contrast in biological second harmonic generation microscopy. Scientific Reports, 7. View this article in WRRO
- Oxygen Mapping of Melanoma Spheroids using Small Molecule Platinum Probe and Phosphorescence Lifetime Imaging Microscopy.. Scientific Reports, 7. View this article in WRRO
- Poly(n-butyl Methacrylate) with Primary Amine End Groups for Supporting Cell Adhesion and Proliferation of Renal Epithelial Cells. International Journal of Polymeric Materials and Polymeric Biomaterials. View this article in WRRO
- Second Harmonic Generation microscopy reveals collagen fibres are more organised in the cervix of postmenopausal women. Reproductive Biology and Endocrinology, 14. View this article in WRRO
- Creating a model of diseased artery damage and failure from healthy porcine aorta. Journal of the Mechanical Behavior of Biomedical Materials, 60, 378-393. View this article in WRRO
- Production and Characterization of a Novel,
Electrospun, Tri-Layer Polycaprolactone Membrane
for the Segregated Co-Culture of Bone and Soft Tissue. Polymers, 8(6), 221-229. View this article in WRRO
- Raman spectroscopy detects melanoma and the tissue surrounding melanoma using tissue-engineered melanoma models. Applied Spectroscopy Reviews, 51(4), 243-257. View this article in WRRO
- Developing repair materials for stress urinary incontinence to withstand dynamic distension. PLoS One, 11(3). View this article in WRRO
- Emulsion templated scaffolds with tunable mechanical properties for bone tissue engineering. Journal of the Mechanical Behavior of Biomedical Materials, 54, 159-172. View this article in WRRO
- Thermoresponsive, stretchable, biodegradable and biocompatible poly(glycerol sebacate)-based polyurethane hydrogels. Polymer Chemistry, 6, 7974-7987. View this article in WRRO
- Rocking Media Over Ex Vivo Corneas Improves This Model and Allows the Study of the Effect of Proinflammatory Cytokines on Wound Healing. Investigative Ophthalmology & Visual Science, 56(3), 1553-1561. View this article in WRRO
- Characterisation of the Physical Composition and Microbial Community Structure of Biofilms within a Model Full-Scale Drinking Water Distribution System. PLOS ONE, 10(2), e0115824-e0115824. View this article in WRRO
- Pulsatile exposure to simulated reflux leads to changes in gene expression in a 3D model of oesophageal mucosa. International Journal of Experimental Pathology, 95(3), 216-228. View this article in WRRO
- Dinuclear ruthenium(II) complexes as two-photon, time-resolved emission microscopy probes for cellular DNA.. Angew Chem Int Ed Engl, 53(13), 3367-3371. View this article in WRRO
- Monitoring fibrous scaffold guidance of three-dimensional collagen organisation using minimally-invasive second harmonic generation.. PLoS One, 9(2), e89761. View this article in WRRO
- NF-κB is activated in oesophageal fibroblasts in response to a paracrine signal generated by acid-exposed primary oesophageal squamous cells.. Int J Exp Pathol, 92(5), 345-356. View this article in WRRO
- The development and characterization of an organotypic tissue-engineered human esophageal mucosal model.. Tissue Eng Part A, 16(3), 1053-1064. View this article in WRRO
- The glycosylation of recombinant human interferon-gamma in Chinese hamster ovary cells..
Chapters
- Fluorescence Microscopy, Optical Techniques in Regenerative Medicine (pp. 29-59).
- Fluorescence Microscopy In Morgan S, Rose FR & Matcher SJ (Ed.), Optical Techniques in Regenerative Medicine (pp. 29-59). Boca Raton: Taylor & Francis.
Conference proceedings papers
- Production of porous polycaprolactone polyHIPEs as substrates for in vitro 3D breast cancer cell culture. TISSUE ENGINEERING PART A, Vol. 29(13-14)
- OPTIMISING THE FABRICATION, SURFACE TREATMENT AND MECHANICAL STIMULATION TO IMPROVE THE CELL PROLIFERATION AND COLLAGEN PRODUCTION FROM PRIMARY DERMAL FIBROBLASTS IN VITRO. TISSUE ENGINEERING PART A, Vol. 29(11-12) (pp 1150-1151)
- MCSS SUPPORT A LAMELLA-LIKE TWISTING ORIENTATION OF COLLAGEN WHEN CULTURED ON ALIGNED ELECTROSPUN POLYCAPROLACTONE FIBRES. TISSUE ENGINEERING PART A, Vol. 29(11-12) (pp 1586-1587)
- ELUCIDATION OF COLLAGEN FIBRE STRUCTURE IN OSTEOGENESIS IMPERFECTA USING SECOND HARMONIC GENERATION IMAGING ON POLYCAPROLACTONE FIBRES. TISSUE ENGINEERING PART A, Vol. 29(11-12) (pp 953-953)
- Development of an in vitro musculoskeletal model using poly (glycerol sebacate methacrylate) (PGSM) polyHIPE-based 3D microporous scaffold encompassing regionally controlled mechanical properties. TISSUE ENGINEERING PART A, Vol. 29(13-14)
- Developing Biodegradable Polymer Resins for Additive Manufacturing via a Novel Synthesis Method. TISSUE ENGINEERING PART A, Vol. 29(13-14)
- A digital reconstruction of the dermal-epidermal junction (DEJ) of the skin in three dimensions. TISSUE ENGINEERING PART A, Vol. 29(13-14)
- Development of an in vitro musculoskeletal model using poly (glycerol sebacate methacrylate) (PGSM) polyHIPE-based 3D microporous scaffold encompassing regionally controlled mechanical properties. TISSUE ENGINEERING PART A, Vol. 29(13-14)
- Development and characterisation of new bioelastomers based on poly(glycerol sebacate)-copolyethylene glycol for biomedical applications. TISSUE ENGINEERING PART A, Vol. 29(13-14)
- A digital reconstruction of the dermal-epidermal junction (DEJ) of the skin in three dimensions. TISSUE ENGINEERING PART A, Vol. 29(13-14)
- Abstract 2122: Visualization of collagen fibre structure in osteogenesis imperfecta using second harmonic generation imaging on polycaprolactone scaffold. Tissue Engineering Part A, Vol. 28(S1) (pp S601-S602). Maastricht, Netherlands, 15 November 2021 - 15 November 2021. View this article in WRRO
- THERE'S MORE TO FAT THAN STEM CELLS, CHARACTERISING FAT GRAFT MATERIALS TO UNDERSTAND THE REGENERATIVE PROPERTIES OF ADIPOSE TISSUES. TISSUE ENGINEERING PART A, Vol. 28 (pp S544-S544)
- Enhanced Collagen Production from Human Dermal Fibroblasts on Poly(glycerol sebacate)-methacrylate Scaffolds. 2018 11th Biomedical Engineering International Conference (BMEiCON), 21 November 2018 - 24 November 2018. View this article in WRRO
- The Use of Second-Harmonic-Generation (SHG) to Assess the Impact of Menopause on the Orientation of Collagen Fibres in the Human Cervix.. REPRODUCTIVE SCIENCES, Vol. 23 (pp 214A-214A)
- Collagen and Mineral Deposition by MLO-A5 Late-Stage Osteoblasts, is Guided by the Fibre Alignment of Electrospun Scaffolds. Proceedings of the 22nd Congress of the European Society of Biomechanics, 10 July 2016 - 13 July 2016.
- Optical measurement of oxygen concentration in melanoma spheroid and skin engineered models. European Cells and Materials (ECM), Vol. Collection 5 (pp 8-8)
- Highly Porous Particles for Cell Recruitment and Delivery in Bone Tissue Engineering. TISSUE ENGINEERING PART A, Vol. 21 (pp S287-S288)
- Barrett's Metaplasia: Exploiting a Tissue-engineered Model to Understand the Response of the Oesophageal Squamous Mucosa to Bile and Acid Exposure. JOURNAL OF PATHOLOGY, Vol. 228 (pp S3-S3)
- Visualising and quantifying the matrix of drinking water biofilms. IWA Biofilms 2011. Shanghai, China, 27 October 2011 - 30 October 2011.
- Invasive behaviour of the OE33 oesophageal adenocarcinoma cell line is dependent on co-operation with fibroblasts.. JOURNAL OF PATHOLOGY, Vol. 222 (pp S43-S43)
- Taurochenodeoxycholic acid activates NF-KB in oesophageal squamous cells without accompanying loss in cell viability. Journal of Pathology, Vol. 220(S1) (pp S11-S11)
- Highly porous polycaprolactone microspheres for skeletal repair promote a mature bone cell phenotype in vitro.. J Mater Chem B.
- Research group
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PhD students
Main Supervisor
- Jeerawan Thanarak
- Meghna Suvarna
Co-supervisor
- Samuel Higginbotham
- Tugba Cebe
- Alice Hann
- Boyang Liu
- Abigail Smith
- Mina Aleemardani
- Jonathan Hinchliffe
- Caitlin Jackson
- Grants
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- Multi-materials additive manufacturing for musculoskeletal applications (principal investigator, Royal Society International Exchanges, £12,000)
- Reversing skin contraction in paediatric burns patients (co-investigator, Sheffield Hospitals Charity, £426,288)
- Transforming metalorganic frameworks promoted bone regeneration through secondary electron hyperspectral imaging (co-investigator, Newton Mobility Grant, £11,930)
- Teaching activities
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I am the programme lead for Biomedical Engineering.
I contribute to learning and teaching within the Department of Materials Science and Engineering and the Faculty of Engineering, teaching on modules based on my research interests (Biology and Chemistry of Living Systems II (MAT2530), Bioimaging (MAT408/6311, Module lead) - fourth year/MSc module.)
I am also the module lead for BIE103 and BIE201, two courses taken by Biomedical engineering students which demonstrate the breadth of the Biomedical engineering field and introduce students to key technical, practical and professional skills within Biomedical engineering