Dr Kang Lan Tee

Books

• Wong TS & Tee KL (2023) Ein praktischer Leitfaden für Protein Engineering. Springer International Publishing.
• Wong TS & Tee KL (2020) A Practical Guide to Protein Engineering. Springer International Publishing.

Journal articles

• Sitompul SN, Diaz Garcia LA, Price J, Tee KL & Wong TS (2024) Fast‐track adaptive laboratory evolution of Cupriavidus necator H16 with divalent metal cations. Biotechnology Journal, 19(7).
• Gonzalez-Perez D, Ratcliffe J, Tan SK, Wong MCM, Yee YP, Nyabadza N, Xu J-H, Wong TS & Tee KL (2021) Random and combinatorial mutagenesis for improved total production of secretory target protein in Escherichia coli. Scientific Reports, 11(1).
• Unrean P, Napathorn SC, Tee KL, Wong TS & Champreda V (2021) Lignin to polyhydroxyalkanoate bioprocessing by novel strain of Pseudomonas monteilii. Biomass Conversion and Biorefinery.
• Johnson AO, Gonzalez-Villanueva M, Wong L, Steinbüchel A, Tee KL, Xu P & Wong TS (2019) Author correction to ‘Design and application of genetically-encoded malonyl-CoA biosensors for metabolic engineering of microbial cell factories’ [Metab. Eng. 44 (2017) 253–264]. Metabolic Engineering.
• Alessa AHA, Tee KL, Gonzalez-Perez D, Omar Ali HEM, Evans CA, Trevaskis A, Xu J-H & Wong TS (2019) Accelerated directed evolution of dye-decolorizing peroxidase using a bacterial extracellular protein secretion system (BENNY). Bioresources and Bioprocessing, 6(1). View this article in WRRO
• Unrean P, Tee KL & Wong TS (2019) Metabolic pathway analysis for in silico design of efficient autotrophic production of advanced biofuels. Bioresources and Bioprocessing, 6(1). View this article in WRRO
• González-Villanueva M, Galaiya H, Staniland P, Staniland J, Savill I, Wong TS & Tee KL (2019) Adaptive laboratory evolution of cupriavidus necator H16 for carbon co-utilization with glycerol. International Journal of Molecular Sciences, 20(22). View this article in WRRO
• Jajesniak P, Tee KL & Wong TS (2019) PTO-QuickStep : a fast and efficient method for cloning random mutagenesis libraries. International Journal of Molecular Sciences, 20(16). View this article in WRRO
• Tee KL, Xu J-H & Wong TS (2019) Protein engineering for bioreduction of carboxylic acids. Journal of Biotechnology, 303, 53-64.
• Johnson AO, Gonzalez-Villanueva M, Tee KL & Wong TS (2018) An Engineered Constitutive Promoter Set with Broad Activity Range for Cupriavidus necator H16. ACS Synthetic Biology, 7(8), 1918-1928. View this article in WRRO
• Tee KL, Grinham J, Othusitse AM, Gonzalez-Villanueva M, Johnson AO & Wong TS (2017) An Efficient Transformation Method for the Bioplastic‐Producing “Knallgas” Bacterium Ralstonia eutropha H16. Biotechnology Journal, 12(11). View this article in WRRO
• Johnson AO, Gonzalez-Villanueva M, Wong L, Steinbüchel A, Tee KL, Xu P & Wong TS (2017) Design and application of genetically-encoded malonyl-CoA biosensors for metabolic engineering of microbial cell factories. Metabolic Engineering, 44, 253-264. View this article in WRRO
• Matthews S, Belcher JD, Tee KL, Girvan HM, McLean KJ, Rigby SE, Levy CW, Leys D, Parker DA, Blankley RT & Munro AW (2017) Catalytic Determinants of Alkene Production by the Cytochrome P450 Peroxygenase OleTJE. The Journal of Biological Chemistry, 292, 5128-5143. View this article in WRRO
• Matthews S, Tee KL, Rattray NJ, McLean KJ, Leys D, Parker DA, Blankley RT & Munro AW (2017) Production of alkenes and novel secondary products by P450 OleT JE using novel H2O2-generating fusion protein systems. FEBS Letters. View this article in WRRO
• McLean KJ, Hans M, Meijrink B, van Scheppingen WB, Vollebregt A, Tee KL, van der Laan J-M, Leys D, Munro AW & van den Berg MA (2015) Single-step fermentative production of the cholesterol-lowering drug pravastatin via reprogramming of Penicillium chrysogenum. Proceedings of the National Academy of Sciences, 112(9), 2847-2852.
• Tee KL & Wong TS (2013) Polishing the craft of genetic diversity creation in directed evolution. Biotechnology Advances, 31(8), 1707-1721. View this article in WRRO
• Kuper J, Tee KL, Wilmanns M, Roccatano D, Schwaneberg U & Wong TS (2012) The role of active-site Phe87 in modulating the organic co-solvent tolerance of cytochrome P450 BM3 monooxygenase. Acta Crystallographica Section F Structural Biology and Crystallization Communications, 68(9), 1013-1017.
• Zhu L, Tee KL, Roccatano D, Sonmez B, Ni Y, Sun Z-H & Schwaneberg U (2010) Directed Evolution of an Antitumor Drug (Arginine Deiminase PpADI) for Increased Activity at Physiological pH. ChemBioChem, 11(5), 691-697.
• Tee KL (2009) Exploring genetic diversity in enzyme engineering using Sequence Saturation Mutagenesis (SeSaM). New Biotechnology, 25, S123-S123.
• Tee KL, Dmytrenko O, Otto K, Schmid A & Schwaneberg U (2008) A p-nitrothiophenolate screening system for the directed evolution of a two-component epoxygenase (StyAB). Journal of Molecular Catalysis B: Enzymatic, 50(2-4), 121-127.
• Seng Wong T, Roccatano D, Loakes D, Tee KL, Schenk A, Hauer B & Schwaneberg U (2008) Transversion-enriched sequence saturation mutagenesis (SeSaM-Tv+): A random mutagenesis method with consecutive nucleotide exchanges that complements the bias of error-prone PCR. Biotechnology Journal, 3(1), 74-82.
• Lan Tee K & Schwaneberg U (2007) Directed Evolution of Oxygenases: Screening Systems, Success Stories and Challenges. Combinatorial Chemistry & High Throughput Screening, 10(3), 197-217.
• Tee KL & Schwaneberg U (2006) A Screening System for the Directed Evolution of Epoxygenases: Importance of Position 184 in P450 BM3 for Stereoselective Styrene Epoxidation. Angewandte Chemie International Edition, 45(32), 5380-5383.
• Schenk A, Wong TS, Roccatano D, Hauer B & Schwaneberg U (2006) Sequence saturation mutagenesis (SeSam) of a gene. BioSpektrum, 12(3), 277-279.
• Wong TS, Tee KL, Hauer B & Schwaneberg U (2005) Sequence saturation mutagenesis with tunable mutation frequencies. Analytical Biochemistry, 341(1), 187-189.
• Wong TS (2004) Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution. Nucleic Acids Research, 32(3), 26e-26.
• Gali VK, Tee KL & Wong TS () Crafting Genetic Diversity: Unlocking the Potential of Protein Evolution. SynBio, 2(2), 142-173.
• Keita VM, Lee YQ, Lakshmanan M, Ow DS-W, Staniland P, Staniland J, Savill I, Tee KL, Wong TS & Lee D-Y () Evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock. Microbial Cell Factories, 23(1).
• Gonzalez-Perez D, Ratcliffe J, Tan SK, Wong MCM, Yee YP, Nyabadza N, Xu J-H, Wong TS & Tee KL () Directed evolution for improved total secretory protein production in Escherichia coli.
• Johari YB, Jaffé SRP, Scarrott JM, Johnson AO, Mozzanino T, Pohle TH, Maisuria S, Bhayat‐Cammack A, Lambiase G, Brown AJ , Tee KL et al () Production of Trimeric SARS‐CoV‐2 Spike Protein by CHO Cells for Serological COVID‐19 Testing. Biotechnology and Bioengineering.
• Tee KL, Roccatano D, Stolte S, Arning J, Jastorff B & Schwaneberg U () Ionic liquid effects on the activity of monooxygenase P450 BM-3. Green Chem., 10(1), 117-123.

Chapters

• Jajesniak P, Tee KL & Wong TS (2022) Rapid Cloning of Random Mutagenesis Libraries Using PTO-QuickStep, Methods in Molecular Biology (pp. 123-135). Springer US
• Keita VM, Gonzalez-Villanueva M, Wong TS & Tee KL (2020) Microbial Utilization of Glycerol for Biomanufacturing, Engineering of Microbial Biosynthetic Pathways (pp. 245-302). Springer Singapore
• Tee KL & Wong TS (2017) Back to Basics: Creating Genetic Diversity, Directed Enzyme Evolution: Advances and Applications (pp. 201-227). Springer International Publishing
• McLean KJ, Luciakova D, Belcher J, Tee KL & Munro AW (2015) Biological Diversity of Cytochrome P450 Redox Partner Systems, Advances in Experimental Medicine and Biology (pp. 299-317). Springer International Publishing
• Tee KL & Wong TS (2014) Directed evolution: A powerful algorithm for advancing synthetic biology. In Singh V (Ed.), Biotechnology Volume 4: Applied Synthetic Biology (pp. 465-497). USA: Studium Press LLC.

Conference proceedings papers

• Munro A, McLean K, Belcher J, Matthews S, Girvan H, Tee K, Poddar H, Andrews A, Leys D, Hans M , van den Berg M et al (2018) Biotechnological applications of cytochrome P450 enzymes. FEBS OPEN BIO, Vol. 8 (pp 21-21)

Other

• Wong TS & Tee KL (2024) Building global health with lego vaccines. Open Access Government, 42(1), 32-33.
• Price J, Wong TS & Tee KL (2024) Microbe development for the biomanufacturing age. Open Access Government, 41(1), 378-379.
• Seng Wong T & Tee KL (2023) CO2 bioeconomy: Creating value from carbon dioxide. Open Access Government, 40(1), 422-423.

Preprints

• Li M, Doudin K, Wong TS & Tee KL (2024) Microbial Synthesis of Polyhydroxyalkanoate Blends with Engineered Pseudomonas Putida, Elsevier BV.
• Gali VK, Tee KL & Wong TS (2023) Crafting Genetic Diversity: Unlocking the Potential of Protein Evolution, MDPI AG.
• Sitompul SN, Price J, Tee KL & Wong TS (2023) Fast-track adaptive laboratory evolution ofCupriavidus necatorH16 with divalent metal cations, Cold Spring Harbor Laboratory.
• Malika Keita V, Lee YQ, Lakshmanan M, Ow DS-W, Staniland P, Staniland J, Savill I, Tee KL, Wong TS & Lee D-Y (2023) Sweetwater: an underrated crude glycerol for sustainable lipid production in non-conventional yeasts, Cold Spring Harbor Laboratory.
• Johari YB, Jaffé SRP, Scarrott JM, Johnson AO, Mozzanino T, Pohle TH, Maisuria S, Bhayat-Cammack A, Brown AJ, Tee KL , Jackson PJ et al () Production of Trimeric SARS-CoV-2 Spike Protein by CHO Cells for Serological COVID-19 Testing, Cold Spring Harbor Laboratory.
• Johari Y, Jaffe S, Scarrott J, Johnson A, Mozzanino TO, Pohle T, Maisuria S, Cammack AB, Brown A, Tee KL , Jackson P et al () Production of Trimeric SARS-CoV-2 Spike Protein by CHO Cells for Serological COVID-19 Testing, Authorea, Inc..
• Tee KL, Jackson PJ, Scarrott JM, Jaffe SRP, Johnson AO, Johari Y, Pohle TH, Mozzanino T, Price J, Grinham J , Brown A et al () Purification of recombinant SARS-CoV-2 spike, its receptor binding domain, and CR3022 mAb for serological assay.