Dr Adam Brown

Journal articles

• Curry E, Sedelnikova S, Rafferty J, Hulley M, Pohle M, Muir G & Brown A (2024) Expanding the RNA polymerase biocatalyst solution space for mRNA manufacture. Biotechnology Journal, 19(6). View this article in WRRO
• O’Neill P, Mistry RK, Brown AJ & James DC (2023) Protein-specific signal peptides for mammalian vector engineering. ACS Synthetic Biology, 12(8), 2339-2352. View this article in WRRO
• Johari YB, Scarrott JM, Pohle TH, Liu P, Mayer A, Brown AJ & James DC (2022) Engineering of the CMV promoter for controlled expression of recombinant genes in HEK293 cells. Biotechnology Journal. View this article in WRRO
• Johnson AO, Fowler SB, Webster CI, Brown AJ & James DC (2022) Bioinformatic design of dendritic cell-specific synthetic promoters. ACS Synthetic Biology, 11(4), 1613-1626.
• Patel YD, Brown AJ, Zhu J, Rosignoli G, Gibson SJ, Hatton D & James DC (2021) Control of Multigene Expression Stoichiometry in Mammalian Cells Using Synthetic Promoters.. ACS Synth Biol.
• Johari YB, Mercer AC, Liu Y, Brown AJ & James DC (2021) Design of synthetic promoters for controlled expression of therapeutic genes in retinal pigment epithelial cells.. Biotechnol Bioeng.
• Cartwright JF, Arnall CL, Patel YD, Barber NOW, Lovelady CS, Rosignoli G, Harris CL, Dunn S, Field RP, Dean G , Daramola O et al (2020) A platform for context-specific genetic engineering of recombinant protein production by CHO cells. Journal of Biotechnology, 312, 11-22.
• Johari YB, Brown AJ, Alves CS, Zhou Y, Wright CM, Estes SD, Kshirsagar R & James DC (2019) CHO Genome Mining for Synthetic Promoter Design. Journal of Biotechnology. View this article in WRRO
• Brown AJ, Gibson SJ, Hatton D, Arnall CL & James DC (2019) Whole synthetic pathway engineering of recombinant protein production. Biotechnology and Bioengineering, 116(2), 375-387. View this article in WRRO
• Brown AJ, Gibson SJ, Hatton D & James DC (2017) In silico design of context-responsive mammalian promoters with user-defined functionality. Nucleic Acids Research, 45(18), 10906-10919. View this article in WRRO
• Brown AJ, Gibson S, Hatton D & James DC (2017) Transcriptome-based identification of the optimal reference CHO genes for normalisation of qPCR data. Biotechnology Journal. View this article in WRRO
• Brown AJ & James DC (2017) Constructing Strong Cell Type-Specific Promoters Through Informed Design, 131-145.
• Brown AJ & James DC (2016) Precision control of recombinant gene transcription for CHO cell synthetic biology. Biotechnology Advances, 34(5), 492-503.
• Brown AJ, Sweeney B, Mainwaring DO & James DC (2015) NF-κB, CRE and YY1 elements are key functional regulators of CMV promoter-driven transient gene expression in CHO cells. Biotechnology Journal, 10(7), 1019-1028.
• Brown AJ, Sweeney B, Mainwaring DO & James DC (2014) Synthetic promoters for CHO cell engineering. Biotechnology and Bioengineering, 111(8), 1638-1647.
• Brown AJ, Mainwaring DO, Sweeney B & James DC (2013) Block decoys: Transcription-factor decoys designed for in vitro gene regulation studies. Analytical Biochemistry, 443(2), 205-210.
• Curry E, Muir G, Qu J, Kis Z, Hulley M, Brown A & Brown A () Engineering an Escherichia coli based in vivo mRNA manufacturing platform. Biotechnology and Bioengineering.
• Sou SN, Harris CL, Williams R, Kozub D, Zurlo F, Patel YD, Kallamvalli Illam Sankaran P, Daramola O, Brown A, James DC , Hatton D et al () CHO synthetic promoters improve expression and product quality of biotherapeutic proteins. Biotechnology Progress. View this article in WRRO
• Johari YB, Jaffé SRP, Scarrott JM, Johnson AO, Mozzanino T, Pohle TH, Maisuria S, Bhayat‐Cammack A, Lambiase G, Brown AJ , Tee KL et al () Production of Trimeric SARS‐CoV‐2 Spike Protein by CHO Cells for Serological COVID‐19 Testing. Biotechnology and Bioengineering.

Chapters

• (2017) Protein Therapeutics Wiley-VCH Verlag GmbH & Co. KGaA
• Brown AJ, Kalsi D, Fernandez-Martell A, Cartwright J, Barber NOW, Patel YD, Turner R, Bryant CL, Johari YB & James DC () Expression Systems for Recombinant Biopharmaceutical Production by Mammalian Cells in Culture, Methods and Principles in Medicinal Chemistry (pp. 423-467). Wiley-VCH Verlag GmbH & Co. KGaA

Patents

• Brown AJ, James DC, Gibson SJ & Hatton D (2018) In silico design of mammalian promoters with user-defined functionality. WO 2018/193072 A1 Appl. 25 Oct 2018.
• James DC & Brown AJ (2015) Synthetic promoters for CHO cells, methods of producing synthetic promoters using transcription factor binding site modules. WO2015079053-A2; WO2015079053-A3 Appl. 08 Oct 2015.
• James DC & Brown AJ (2014) Regulating recombinant gene expression in vitro comprises providing a host cell encoding recombinant genes for expression, and contacting the cell with an exonuclease resistant block decoy. WO2014202640-A1 Appl. 24 Dec 2014.

Preprints

• Alexandru-Crivac CN, Cartwright JF, Taylor R, Sweeney BM, Feary M, Chathoth KT, Fabian DK, Allsopp H, Brown AJ & James DC (2024) Large-Scale Comparative Transcriptomic Analysis of CHO Cell Functional Adaptation to Recombinant Monoclonal Antibody Production, Cold Spring Harbor Laboratory.
• Curry E, Muir G, Qu J, Kis Z, Hulley M & Brown A (2023) Engineering an E. coli -based in vivo mRNA manufacturing platform, Authorea, Inc..
• O’Neill P, Mistry RK, Brown AJ & James DC (2023) Protein-specific signal peptides for mammalian vector engineering, Cold Spring Harbor Laboratory.
• Johari YB, Jaffé SRP, Scarrott JM, Johnson AO, Mozzanino T, Pohle TH, Maisuria S, Bhayat-Cammack A, Brown AJ, Tee KL , Jackson PJ et al () Production of Trimeric SARS-CoV-2 Spike Protein by CHO Cells for Serological COVID-19 Testing, Cold Spring Harbor Laboratory.