Professor Mike Williamson
School of Biosciences
Personal Chair
m.williamson@sheffield.ac.uk
+44 114 222 4224
+44 114 222 4224
Firth Court
Full contact details
Professor Mike Williamson
School of Biosciences
Firth Court
Western Bank
Sheffield
S10 2TN
School of Biosciences
Firth Court
Western Bank
Sheffield
S10 2TN
- Profile
-
My research concentrates on protein structure and function, mainly by means of NMR, and is described in more detail below.
I also teach NMR and protein structure, signalling, membranes and molecular motors, as well as numerical and statistical methods. I was a reviewer for the HEFCE QAA Molecular Biosciences reviews in 1998-2000, and coincidentally led the MBB submission, in which we got 24/24.
I also headed up the departmental Independent Evaluation of Teaching in 2008, which was also highly complimentary of our teaching. I have been involved in a number of University committees, mostly on admissions, finance and personnel.
I was (2009-2011) Chair of the UK NMR discussion group; also (2009-2012) Chair of the Biochemical Society theme panel II (Molecular structure and function); (2009-2013) a member of BBSRC Committee D (Molecules, Cells and Industrial Biotechnology); (2005-2009) secretary of Euromar; and (2018-) a member of Council of the International Conference on Magnetic Resonance in Biological Systems (ICMRBS).
I was on sabbatical in Osaka, Japan from September 2008 until September 2009, mainly to write a book, entitled How Proteins Work, published by Garland Press in July 2011 and available online and in all good bookshops. Also available in Italian and Japanese translations.
Career history
- 1975-1978 Natural Sciences, Clare College, Cambridge University (I)
- 1978-1981 PhD "Structural Studies on Some Antibiotics", supervised by Dudley Williams
- 1981-1984 Junior Research Fellow, Churchill College, Cambridge
- 1992-1983 SERC/NATO overseas research fellow, ETH Zürich, with Kurt Wüthrich
- 1984-1990 Team leader, Bio-NMR, Roche Products Ltd, Welwyn Garden City
- 1990-current University of Sheffield (appointed Professor in 2001)
- 2017-2021 Head of Department of Molecular Biology and Biotechnology
- 1995 Fellow of the Royal Society of Chemistry and chartered chemist
- 1997 Japan Society for the Promotion of Science (JSPS) invitation fellow
- 2001 ScD, University of Cambridge
- 2008-9 Visiting professor, Kinki University, Japan
- 2009 Visiting professor, Osaka University, Japan
- 2015 Special invited professor, Kyoto University, Japan
- Research interests
-
During my PhD I used NMR to look at the structure and interactions of antibiotics mainly related to vancomycin, still a vital drug in the constant battle against bacterial drug resistance. This led to an interest in the NOE, where I worked first on 1D NOEs, showing that by using a viscous solvent you can make small molecules behave like bigger ones, and determined the definitive structure of vancomycin.
Around this time, Wüthrich was developing 2D NMR as a way of studying proteins, so after my PhD I got a research fellowship to work in his lab, where I was lucky enough to work on the first NMR structure of a globular protein (see his 2002 Nobel Prize lecture).
Since then, I have worked both on NMR methodology and on determination of protein structures by NMR. In methodology, I have worked in four main areas:
- The nuclear Overhauser effect (NOE)
- Chemical shifts in proteins
- Relaxation
- Study of proteins using high pressure
- Publications
-
Show: Featured publications All publications
Featured publications
Journal articles
- Autocatalytic selection as a driver for the origin of life. Life, 14(5). View this article in WRRO
- Protein binding: a fuzzy concept. Life, 13(4).
- The measurement of binding affinities by NMR chemical shift perturbation. Journal of Biomolecular NMR, 76(4), 153-163. View this article in WRRO
- The accuracy of protein structures in solution determined by AlphaFold and NMR. Structure, 30(7), 925-933.e2.
- The accuracy of NMR protein structures in the Protein Data Bank. Structure, 29(12), 1430-1439.e2.
- ‘Carbon-monoxide-releasing molecule-2 (CORM-2)’ is a misnomer: ruthenium toxicity, not CO release, accounts for Its antimicrobial effects. Antioxidants, 10(6). View this article in WRRO
- A method for validating the accuracy of NMR protein structures. Nature Communications, 11(-), ---. View this article in WRRO
- Two-site recognition of Staphylococcus aureus peptidoglycan by lysostaphin SH3b. Nature Chemical Biology. View this article in WRRO
- A thiol-reactive Ru(II) ion, not CO release, underlies the potent antimicrobial and cytotoxic properties of CO-releasing molecule-3. Redox Biology, 18, 114-123. View this article in WRRO
- Molecular Mechanism for the Hofmeister Effect Derived from NMR and DSC Measurements on Barnase. ACS Omega, 1(4), 669-679. View this article in WRRO
- Molecular basis for bacterial peptidoglycan recognition by LysM domains.. Nat Commun, 5, 4269. View this article in WRRO
- Detection of salt bridges to lysines in solution in barnase.. Chem Commun (Camb), 49(84), 9824-9826.
- Using chemical shift perturbation to characterise ligand binding.. Prog Nucl Magn Reson Spectrosc, 73, 1-16.
- Pressure-induced chemical shifts as probes for conformational fluctuations in proteins. Progress in Nuclear Magnetic Resonance Spectroscopy, 71, 35-58.
- Pressure-dependent structure changes in barnase on ligand binding reveal intermediate rate fluctuations.. Biophys J, 97(5), 1482-1490.
- Molecular model for astringency produced by polyphenol/protein interactions.. Biomacromolecules, 5(3), 942-949.
- Polyphenol/peptide binding and precipitation.. J Agric Food Chem, 50(6), 1593-1601.
- The importance of being proline: the interaction of proline-rich motifs in signaling proteins with their cognate domains. FASEB J, 14(2), 231-241.
- Temperature dependence of 1H chemical shifts in proteins.. J Biomol NMR, 9(4), 359-369.
- The structure and function of proline-rich regions in proteins.. Biochem J, 297 ( Pt 2), 249-260.
- SOLUTION CONFORMATION OF PROTEINASE INHIBITOR-IIA FROM BULL SEMINAL PLASMA BY H-1 NUCLEAR MAGNETIC-RESONANCE AND DISTANCE GEOMETRY. J MOL BIOL, 182(2), 295-315.
- STRUCTURE REVISION OF THE ANTIBIOTIC VANCOMYCIN - THE USE OF NUCLEAR OVERHAUSER EFFECT DIFFERENCE SPECTROSCOPY. J AM CHEM SOC, 103(22), 6580-6585.
All publications
Books
- How Proteins Work. Garland Science.
- Chapter 3 Applications of the NOE in Molecular Biology.
- The Nuclear Overhauser Effect in Structural and Conformational Analysis. Wiley.
Journal articles
- Dissecting the Enterococcal Polysaccharide Antigen (EPA) structure to explore innate immune evasion and phage specificity. Carbohydrate Polymers, 347, 122686-122686.
- Autocatalytic selection as a driver for the origin of life. Life, 14(5). View this article in WRRO
- Clostridioides difficile canonical L,D-transpeptidases catalyze a novel type of peptidoglycan cross-links and are not required for beta-lactam resistance. Journal of Biological Chemistry, 300(1), 105529-105529.
- A review on the structure of Bombyx mori silk fibroin fiber studied using solid-state NMR: An antipolar lamella with an 8-residue repeat. International Journal of Biological Macromolecules, 245, 125537-125537.
- Improved methodology for protein NMR structure calculation using hydrogen bond restraints and ANSURR validation: The SH2 domain of SH2B1. Structure, 31(8), 975-986.e3. View this article in WRRO
- Protein binding: a fuzzy concept. Life, 13(4).
- Bioinformatic analysis of WxL domain proteins. Saudi Journal of Biological Sciences, 30(2).
- The measurement of binding affinities by NMR chemical shift perturbation. Journal of Biomolecular NMR, 76(4), 153-163. View this article in WRRO
- 1H, 13C, and 15N resonance assignments of a conserved putative cell wall binding domain from Enterococcus faecalis. Biomolecular NMR Assignments, 1-5.
- Utilization and accumulation of compatible solutes in Halomonas pacifica: a species of moderately halophilic bacteria isolated from a saline lake in South Libya. Access Microbiology, 4(5). View this article in WRRO
- The accuracy of protein structures in solution determined by AlphaFold and NMR. Structure, 30(7), 925-933.e2.
- The accuracy of NMR protein structures in the Protein Data Bank. Structure, 29(12), 1430-1439.e2.
- ‘Carbon-monoxide-releasing molecule-2 (CORM-2)’ is a misnomer: ruthenium toxicity, not CO release, accounts for Its antimicrobial effects. Antioxidants, 10(6). View this article in WRRO
- Probiotic properties of Lactobacillus helveticus and Lactobacillus plantarum isolated from traditional Pakistani yoghurt. BioMed Research International, 2020, 1-17. View this article in WRRO
- A method for validating the accuracy of NMR protein structures. Nature Communications, 11(-), ---. View this article in WRRO
- Chain-folded lamellar structure and dynamics of the crystalline fraction of Bombyx mori silk fibroin and of (Ala-Gly-Ser-Gly-Ala-Gly)n model peptides. International Journal of Biological Macromolecules, 164, 3974-3983.
- Characterisation of bacteriocins produced by Lactobacillus spp. isolated from the traditional Pakistani yoghurt and their antimicrobial activity against common foodborne pathogens. BioMed Research International, 2020. View this article in WRRO
- Conformational exchange in the potassium channel blocker ShK. Scientific Reports, 9(1). View this article in WRRO
- Two-site recognition of Staphylococcus aureus peptidoglycan by lysostaphin SH3b. Nature Chemical Biology. View this article in WRRO
- Paramagnetic relaxation enhancement‐assisted structural characterization of a partially disordered conformation of ubiquitin. Protein Science, 28(11), 1993-2003. View this article in WRRO
- Decoration of the enterococcal polysaccharide antigen EPA is essential for virulence, cell surface charge and interaction with effectors of the innate immune system. PLoS Pathogens , 15(5). View this article in WRRO
- Drawing single NMR spins and understanding relaxation. Natural Product Communications, 14(5). View this article in WRRO
- Structural investigation into the threading intercalation of a chiral dinuclear ruthenium(II) polypyridyl complex through a B-DNA oligonucleotide. Journal of the American Chemical Society, 141(11), 4644-4652. View this article in WRRO
- Why the energy landscape of barnase is hierarchical. Frontiers in Molecular Biosciences, 5. View this article in WRRO
- Characterization of low-lying excited states of proteins by high-pressure NMR. Biochimica et Biophysica Acta - Proteins and Proteomics. View this article in WRRO
- A thiol-reactive Ru(II) ion, not CO release, underlies the potent antimicrobial and cytotoxic properties of CO-releasing molecule-3. Redox Biology, 18, 114-123. View this article in WRRO
- Structure of a Wbl protein and implications for NO sensing by M. tuberculosis. Nature Communications, 8(1). View this article in WRRO
- Pressure-dependent chemical shifts in the R3 domain of Talin show that It Is thermodynamically poised for binding to either vinculin or RIAM. Structure, 25(12), 1856-1866. View this article in WRRO
- Conserved asymmetry underpins homodimerization of Dicer-associated double-stranded RNA-binding proteins. Nucleic Acids Research, 45(21), 12577-12584. View this article in WRRO
- MYT1L mutations cause intellectual disability and variable obesity by dysregulating gene expression and development of the neuroendocrine hypothalamus. PLoS Genetics, 13(8). View this article in WRRO
- Multi-site binding of epigallocatechin gallate to human serum albumin measured by NMR and isothermal titration calorimetry. Bioscience Reports, 37(3). View this article in WRRO
- Molecular Mechanism for the Hofmeister Effect Derived from NMR and DSC Measurements on Barnase. ACS Omega, 1(4), 669-679. View this article in WRRO
- Homeostasis of metabolites in Escherichia coli on transition from anaerobic to aerobic conditions and the transient secretion of pyruvate. Royal Society Open Science, 3(8), 160187-160187. View this article in WRRO
- Ferrous Iron Binding Key to Mms6 Magnetite Biomineralisation: A Mechanistic Study to Understand Magnetite Formation Using pH Titration and NMR Spectroscopy. Chemistry - A European Journal, 22(23), 7885-7894. View this article in WRRO
- The Polycystin-1, Lipoxygenase, and α-Toxin Domain Regulates Polycystin-1 Trafficking. Journal of the American Society of Nephrology, 27(4), 1159-1173.
- Synthesis of Chlorophyll-Binding Proteins in a Fully Segregated Δycf54 Strain of the Cyanobacterium Synechocystis PCC 6803. Frontiers in Plant Science, 7. View this article in WRRO
- Backbone assignment and secondary structure of the PLAT domain of human polycystin-1. Biomolecular NMR Assignments, 9(2), 369-373.
- Analysis of the Structure of Bombyx mori Silk Fibroin by NMR. Macromolecules, 48(8), 2345-2357.
- Intermolecular Packing in B. mori Silk Fibroin: Multinuclear NMR Study of the Model Peptide (Ala-Gly)15 Defines a Heterogeneous Antiparallel Antipolar Mode of Assembly in the Silk II Form. Macromolecules, 48(1), 28-36. View this article in WRRO
- Pressure-Dependent Conformation and Fluctuation in Folded Protein Molecules, 109-127.
- Molecular basis for bacterial peptidoglycan recognition by LysM domains.. Nat Commun, 5, 4269. View this article in WRRO
- Local Structure and Dynamics of Serine in the Heterogeneous Structure of the Crystalline Domain of Bombyx mori Silk Fibroin in Silk II Form Studied by 2D 13C–13C Homonuclear Correlation NMR and Relaxation Time Observation. Macromolecules, 47(13), 4308-4316.
- Close Identity between Alternatively Folded State N-2 of Ubiquitin and the Conformation of the Protein Bound to the Ubiquitin-Activating Enzyme. BIOCHEMISTRY, 53(3), 447-449.
- Corrigendum to "Using chemical shift perturbation to characterise ligand binding" [Prog. Nucl. Magn. Reson. Spectrosc. 73C (2013) 1-16 (DOI:10.1016/j.pnmrs.2013.02.001). Progress in Nuclear Magnetic Resonance Spectroscopy.
- Structural studies on dinuclear ruthenium(II) complexes that bind diastereoselectively to an antiparallel folded human telomere sequence.. J Med Chem, 56(21), 8674-8683.
- Detection of salt bridges to lysines in solution in barnase.. Chem Commun (Camb), 49(84), 9824-9826.
- Using chemical shift perturbation to characterise ligand binding.. Prog Nucl Magn Reson Spectrosc, 73, 1-16.
- Pressure-induced chemical shifts as probes for conformational fluctuations in proteins. Progress in Nuclear Magnetic Resonance Spectroscopy, 71, 35-58.
- Solution structure of the Q41N variant of ubiquitin as a model for the alternatively folded N
2 state of ubiquitin. Biochemistry, 52(11), 1874-1885.
- Structure and function of a bacterial Fasciclin I Domain Protein elucidates function of related cell adhesion proteins such as TGFBIp and periostin. FEBS Open Bio, 3, 71-77. View this article in WRRO
- 2P008 Solution structure of the "pure" high-energy state of ubiquitin: Q41N at 2.5 kbar(01A. Protein: Structure,Poster). Seibutsu Butsuri, 53(supplement1-2), s160.
- Intrinsically disordered proteins: administration not executive.. Biochem Soc Trans, 40(5), 945-949.
- 1PT112 Solution stucture of the alternatively folded state of ubiquitin(The 50th Annual Meeting of the Biophysical Society of Japan). Seibutsu Butsuri, 52(supplement), S87-S87.
- Two Different Packing Arrangements of Antiparallel Polyalanine. Angewandte Chemie, 124(5), 1238-1241.
- In support of the BMRB. Nature Structural & Molecular Biology, 19(9), 854-860.
- Two Different Packing Arrangements of Antiparallel Polyalanine. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 51(5), 1212-1215.
- Amide temperature coefficients in the protein G B1 domain.. J Biomol NMR, 52(1), 57-64.
- Reprogramming of escherichia coli K-12 metabolism during the initial phase of transition from an anaerobic to a micro-aerobic environment. PLoS ONE, 6(9). View this article in WRRO
- ChemInform Abstract: NOESY. ChemInform, 42(18), no-no.
- Ab Initio Calculations of Deuterium Isotope Effects on Chemical Shifts of Salt-Bridged Lysines. J PHYS CHEM B, 115(12), 3208-3215.
- Amide temperature coefficients in the protein G B1 domain. Journal of Biomolecular NMR, 1-8.
- Experimental evidence that the membrane-spanning helix of PufX adopts a bent conformation that facilitates dimerisation of the Rhodobacter sphaeroides RC-LH1 complex through N-terminal interactions.. Biochim Biophys Acta, 1807(1), 95-107.
- Protein-protein interactions.. Biochem Soc Trans, 38(4), 875-878.
- Signature Active Site Architectures Illuminate the Molecular Basis for Ligand Specificity in Family 35 Carbohydrate Binding Module. BIOCHEMISTRY-US, 49(29), 6193-6205.
- Structural origins of pH-dependent chemical shifts in the B1 domain of protein G.. Proteins, 78(14), 3000-3016.
- Differentiating quadruplexes: binding preferences of a luminescent dinuclear ruthenium(II) complex with four-stranded DNA structures.. Org Biomol Chem, 8(11), 2617-2621.
- Inside Cover: Structure of the Complex of [Ru(tpm)(dppz)py]2+with a B-DNA Oligonucleotide-A Single-Substituent Binding Switch for a Metallo-Intercalator (Chem. Eur. J. 8/2010). Chemistry - A European Journal, 16(8), 2324-2324.
- Structure of the complex of [Ru(tpm)(dppz)py](2+) with a B-DNA oligonucleotide - a single-substituent binding switch for a metallo-intercalator.. Chemistry, 16(8), 2407-2417.
- A polycystin-2 (TRPP2) dimerization domain essential for the function of heteromeric polycystin complexes. EMBO J, 29(7), 1176-1191.
- Structural analysis of the binding of the diquaternary pyridophenazine derivative dqdppn to B-DNA oligonucleotides.. Org Biomol Chem, 8(3), 648-654.
- Dimerization of protein G B1 domain at low pH: a conformational switch caused by loss of a single hydrogen bond.. Proteins, 78(7), 1652-1661.
- Dimerisation of the UBA Domain of p62 Inhibits Ubiquitin Binding and Regulates NF-kappa B Signalling. J MOL BIOL, 396(1), 178-194.
- Pressure-dependent structure changes in barnase on ligand binding reveal intermediate rate fluctuations.. Biophys J, 97(5), 1482-1490.
- Intra- and Intermolecular Effects on H-1 Chemical Shifts in a Silk Model Peptide Determined by High-Field Solid State H-1 NMR and Empirical Calculations. J PHYS CHEM B, 113(29), 9756-9761.
- Pressure-dependent 13C chemical shifts in proteins: origins and applications.. J Biomol NMR, 44(1), 25-33. View this article in WRRO
- Characterization of salt bridges to lysines in the protein G B1 domain.. J Am Chem Soc, 131(13), 4674-4684.
- The interaction of amyloid A beta(1-40) with lipid bilayers and ganglioside as studied by P-31 solid-state NMR. CHEM PHYS LIPIDS, 158(1), 54-60. View this article in WRRO
- Automated protein structure calculation from NMR data.. J Biomol NMR, 43(3), 131-143. View this article in WRRO
- Use of quantitative (1)H NMR chemical shift changes for ligand docking into barnase.. J Biomol NMR, 43(1), 11-19.
- Structural change in a B-DNA helix with hydrostatic pressure.. Nucleic Acids Res, 36(12), 4032-4037. View this article in WRRO
- Pressure-induced changes in the solution structure of the GB1 domain of protein G.. Proteins, 71(3), 1432-1440.
- Binding of topotecan to a nicked DNA oligomer in solution. CHEM-EUR J, 14(9), 2788-2794.
- Characterization of a double dockerin from the cellulosome of the anaerobic fungus Piromyces equi. J MOL BIOL, 373(3), 612-622.
- NMR assignment of the Rhodobacter sphaeroides fasciclin-1 domain protein (Fdp). BIOMOL NMR ASSIGM, 1(1), 11-12. View this article in WRRO
- Novel xylan-binding properties of an engineered family 4 carbohydrate-binding module. Biochemical Journal, 406(2), 209-214.
- The solution structure of the PufX polypeptide from Rhodobacter sphaeroides.. FEBS Lett, 580(30), 6967-6971.
- Epigallocatechin gallate, the main polyphenol in green tea, binds to the T-cell receptor, CD4: Potential for HIV-1 therapy.. J Allergy Clin Immunol, 118(6), 1369-1374.
- Binding of amyloid beta-peptide to ganglioside micelles is dependent on histidine-13.. Biochem J, 397(3), 483-490.
- Activation of the global gene regulator PrrA (RegA) from Rhodobacter sphaeroides.. Biochemistry, 45(25), 7872-7881.
- Noncovalent cross-linking of casein by epigallocatechin gallate characterized by single molecule force microscopy.. J Agric Food Chem, 54(12), 4077-4081.
- The Campylobacter jejuni PEB1a adhesin is an aspartate/glutamate-binding protein of an ABC transporter essential for microaerobic growth on dicarboxylic amino acids.. Mol Microbiol, 60(5), 1262-1275.
- Redox-responsive in vitro modulation of the signalling state of the isolated PrrB sensor kinase of Rhodobacter sphaeroides NCIB 8253. FEBS LETT, 580(13), 3206-3210.
- An experimental investigation of conformational fluctuations in proteins G and L.. Structure, 13(11), 1677-1684.
- Creaming in black tea.. J Agric Food Chem, 53(20), 7997-8002.
- Probing the mechanism of ligand recognition in family 29 carbohydrate-binding modules. J BIOL CHEM, 280(25), 23718-23726.
- Systems biology: will it work?. Biochem Soc Trans, 33(Pt 3), 503-506.
- Available carbon source influences the resistance of Neisseria meningitidis against complement. Journal of Experimental Medicine, 201(10), 1637-1645. View this article in WRRO
- Structure of a mannan-specific family 35 carbohydrate-binding module: evidence for significant conformational changes upon ligand binding.. J Mol Biol, 347(2), 287-296.
- Multiple binding modes of the camptothecin family to DNA oligomers. CHEM-EUR J, 10(22), 5776-5787.
- Molecular model for astringency produced by polyphenol/protein interactions.. Biomacromolecules, 5(3), 942-949.
- Green tea polyphenol epigallocatechin gallate binding to CD4 as a model for the inhibition of HIV-1-gp120 binding to CD4+ T cells. Journal of Allergy and Clinical Immunology, 113(2), S256-S256.
- Copper-mediated LDL oxidation by homocysteine and related compounds depends largely on copper ligation.. Biochim Biophys Acta, 1688(1), 33-42.
- Solution structure and DNA binding of the effector domain from the global regulator PrrA (RegA) from Rhodobacter sphaeroides: insights into DNA binding specificity.. Nucleic Acids Res, 31(23), 6778-6787.
- Many residues in cytochrome c populate alternative states under equilibrium conditions.. Proteins, 53(3), 731-739.
- The solution structure of bovine pancreatic trypsin inhibitor at high pressure.. Protein Sci, 12(9), 1971-1979.
- The modular organisation and stability of a thermostable family 10 xylanase. BIOCATAL BIOTRANSFOR, 21(4-5), 253-260.
- Pressure-dependent changes in the solution structure of hen egg-white lysozyme.. J Mol Biol, 327(4), 857-865.
- Orientation of 1,3-bisphosphoglycerate analogs bound to phosphoglycerate kinase.. J Biol Chem, 278(13), 10957-10962.
- The plasticity of proteins probed by NMR chemical shifts : effects of pressure and temperature. Seibutsu Butsuri, 43(supplement), S7-S7.
- LytG of Bacillus subtilis is a novel peptidoglycan hydrolase: the major active glucosaminidase.. Biochemistry, 42(2), 257-264.
- Importance of hydrophobic and polar residues in ligand binding in the family 15 carbohydrate-binding module from Cellvibrio japonicus Xyn10C. Biochemistry, 42(31), 9316-9323.
- Multiple conformations of the proline-rich protein/epigallocatechin gallate complex determined by time-averaged nuclear Overhauser effects.. J Am Chem Soc, 124(33), 9899-9905.
- Expression, purification and characterisation of full-length histidine protein kinase RegB from Rhodobacter sphaeroides. J MOL BIOL, 320(2), 201-213.
- Calcium binding and thermostability of carbohydrate binding module CBM4-2 of Xyn10A from Rhodothermus marinus. BIOCHEMISTRY-US, 41(18), 5720-5729.
- The solution structure of the CBM4-2 carbohydrate binding module from a thermostable Rhodothermus marinus xylanase.. Biochemistry, 41(18), 5712-5719.
- Polyphenol/peptide binding and precipitation.. J Agric Food Chem, 50(6), 1593-1601.
- Letter to the Editor: Virtually complete H-1, C-13 and N-15 resonance assignments of the second family 4 xylan binding module of Rhodothermus marinus xylanase 10A. J BIOMOL NMR, 22(2), 187-188.
- Chemical dreams. CHEM BRIT, 38(1), 19-19.
- Characterization of a cellulosome dockerin domain from the anaerobic fungus Piromyces equi.. Nat Struct Biol, 8(9), 775-778.
- Clostridium thermocellum Xyn10B carbohydrate-binding module 22-2: The role of conserved amino acids in ligand binding. BIOCHEMISTRY-US, 40(31), 9167-9176.
- An NMR and enzyme study of the carbon metabolism of Neisseria meningitidis.. Microbiology, 147(Pt 6), 1473-1482.
- Role of hydrogen bonding in the interaction between a xylan binding module and xylan. BIOCHEMISTRY-US, 40(19), 5700-5707.
- A nicked duplex decamer DNA with a PEG(6) tether. NUCLEIC ACIDS RES, 29(5), 1132-1143.
- Evidence for synergy between family 2b carbohydrate binding modules in Cellulomonas fimi xylanase 11A. BIOCHEMISTRY-US, 40(8), 2468-2477.
- Pressure-dependent changes in the structure of the melittin alpha-helix determined by NMR. J BIOMOL NMR, 19(2), 115-124.
- The structural basis for the ligand specificity of family 2 carbohydrate-binding modules.. J Biol Chem, 275(52), 41137-41142.
- Structure determination of [Arg8]vasopressin methylenedithioether in dimethylsulfoxide using NMR. EUR J BIOCHEM, 267(14), 4504-4510.
- The solution structure of Rhodobacter sphaeroides LH1beta reveals two helical domains separated by a more flexible region: structural consequences for the LH1 complex.. J Mol Biol, 298(1), 83-94.
- Trp22, Trp24, and Tyr8 play a pivotal role in the binding of the family 10 cellulose-binding module from Pseudomonas xylanase A to insoluble ligands. BIOCHEMISTRY-US, 39(5), 985-991.
- Solution structure of the CBM10 cellulose binding module from Pseudomonas xylanase A.. Biochemistry, 39(5), 978-984.
- The importance of being proline: the interaction of proline-rich motifs in signaling proteins with their cognate domains. FASEB J, 14(2), 231-241.
- An advantage for use of isotope labeling and NMR chemical shifts to analyze the structure of four homologous IgG-binding domains of staphylococcal protein A. J BIOCHEM BIOPH METH, 42(1-2), 35-47.
- Carbohydrate-binding modules from a thermostable Rhodothermus marinus xylanase: cloning, expression and binding studies. BIOCHEM J, 345, 53-60.
- Structure and dynamics of photosynthetic membrane-bound proteins in Rhodobacter Sphaeroides, studied with solid-state NMR spectroscopy. PHOTOSYNTH RES, 63(3), 259-267.
- The self-association of the black tea polyphenol theaflavin and its complexation with caffeine. J CHEM SOC PERK T 2(2), 317-322.
- Carbohydrate-binding modules from a thermostable Rhodothermus marinus xylanase: cloning, expression and binding studies. Biochemical Journal, 345(1), 53-53.
- The type II and X cellulose-binding domains of Pseudomonas xylanase A potentiate catalytic activity against complex substrates by a common mechanism. BIOCHEM J, 342, 473-480.
- The type II and X cellulose-binding domains of Pseudomonas xylanase A potentiate catalytic activity against complex substrates by a common mechanism. Biochemical Journal, 342(2), 473-473.
- A family IIb xylan-binding domain has a similar secondary structure to a homologous family IIa cellulose-binding domain but different ligand specificity.. Structure, 7(7), 853-864.
- Solid phase synthesis and biological activities of [Arg(8)]-vasopressin methylenedithioether. BIOORG MED CHEM LETT, 9(13), 1767-1772.
- Analysis of peptidoglycan structure from vegetative cells of Bacillus subtilis 168 and role of PBP 5 in peptidoglycan maturation.. J Bacteriol, 181(13), 3956-3966.
- A light-harvesting antenna protein retains its folded conformation in the absence of protein-lipid and protein-pigment interactions. BIOPOLYMERS, 49(5), 361-372.
- The starch-binding domain from glucoamylase disrupts the structure of starch.. FEBS Lett, 447(1), 58-60.
- C-alpha and C-beta carbon-13 chemical shifts in proteins from an empirical database. J BIOMOL NMR, 13(3), 199-211.
- Stereochemical model of [2+2]cycloaddition of chlorosulfonyl isocyanate to chiral vinyl ethers. J CHEM SOC PERK T 2(2), 217-224.
- Highly potent bisphosphonate ligands for phosphoglycerate kinase and protein binding studies. PHOSPHORUS SULFUR AND SILICON AND THE RELATED ELEMENTS, 146, 533-536.
- Characterisation of low free-energy excited states of folded proteins.. J Mol Biol, 284(5), 1625-1639.
- Highly potent bisphosphonate ligands for phosphoglycerate kinase.. J Med Chem, 41(23), 4439-4452.
- The structure of the melittin tetramer at different temperatures - An NOE-based calculation with chemical shift refinement. EUR J BIOCHEM, 257(2), 479-487.
- Synthesis and binding of stable bisubstrate ligands for phosphoglycerate kinase.. Bioorg Med Chem Lett, 8(18), 2603-2608.
- Peptidoglycan structural dynamics during germination of Bacillus subtilis 168 endospores.. J Bacteriol, 180(17), 4603-4612.
- All three surface tryptophans in Type IIa cellulose binding domains play a pivotal role in binding both soluble and insoluble ligands. FEBS LETT, 429(3), 312-316.
- Pseudomonas cellulose-binding domains mediate their effects by increasing enzyme substrate proximity. BIOCHEM J, 331, 775-781.
- GENFOLD: a genetic algorithm for folding protein structures using NMR restraints.. Protein Sci, 7(2), 491-499.
- Structure and conformation of the procyanidin B-2 dimer. MAGN RESON CHEM, 35(12), 854-858.
- Towards stereochemical and conformational assignment in flexible molecules using NOEs and molecular modelling. J CHEM SOC PERK T 2(9), 1811-1818.
- Function of conserved tryptophans in the Aspergillus niger glucoamylase 1 starch binding domain.. Biochemistry, 36(24), 7535-7539.
- Temperature dependence of 1H chemical shifts in proteins.. J Biomol NMR, 9(4), 359-369.
- The effect of ring currents on carbon chemical shifts in cytochromes.. J Biomol NMR, 9(4), 389-395.
- Solution structure of the granular starch binding domain of Aspergillus niger glucoamylase bound to beta-cyclodextrin.. Structure, 5(5), 647-661.
- Multiple interactions between polyphenols and a salivary proline-rich protein repeat result in complexation and precipitation.. Biochemistry, 36(18), 5566-5577.
- Inhibition of growth of Dictyostelium discoideum amoebae by bisphosphonate drugs is dependent on cellular uptake.. Pharm Res, 14(5), 625-630.
- Protein chemical shifts., 60, 53-69.
- NMR study of silk I structure of Bombyx mori silk fibroin with N-15- and C-13-NMR chemical shift contour plots. BIOPOLYMERS, 41(2), 193-203.
- Protein chemical shifts.. Methods Mol Biol, 60, 53-69.
- Human cytochrome P450 3A4 is involved in the biotransformation of the herbicide 2,4-dichlorophenoxyacetic acid. ENVIRON TOXICOL PHAR, 2(4), 397-401.
- Metabolism of organochlorine pesticides: the role of human cytochrome P450 3A4.. Chemosphere, 33(4), 759-769.
- Solution structure of the granular starch binding domain of glucoamylase from Aspergillus niger by nuclear magnetic resonance spectroscopy.. J Mol Biol, 259(5), 970-987.
- Tannin interactions with a full-length human salivary proline-rich protein display a stronger affinity than with single proline-rich repeats.. FEBS Lett, 382(3), 289-292.
- Stacking interactions between caffeine and methyl gallate. J CHEM SOC FARADAY T, 92(2), 231-234.
- Synthesis and kinetic studies of bisubstrate analogues of phosphoglycerate kinase. COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS, 61, S88-S91.
- THE RELATIONSHIP BETWEEN AMIDE PROTON CHEMICAL-SHIFTS AND SECONDARY STRUCTURE IN PROTEINS. J BIOMOL NMR, 6(3), 227-236.
- 1H and 15N assignments and secondary structure of the starch-binding domain of glucoamylase from Aspergillus niger.. Eur J Biochem, 233(2), 568-578.
- Expression in Aspergillus niger of the starch-binding domain of glucoamylase. Comparison with the proteolytically produced starch-binding domain.. Eur J Biochem, 233(2), 561-567.
- SEARCHING CONFORMATIONAL SPACE IN FLEXIBLE MOLECULES USING NOES AND MOLECULAR MODELING. J ORG CHEM, 60(11), 3533-3538.
- Application of 1H NMR chemical shifts to measure the quality of protein structures.. J Mol Biol, 247(4), 541-546.
- NMR-STUDIES OF DEXTRAN OLIGOMER INTERACTIONS WITH MODEL POLYPHENOLS. CARBOHYD RES, 266(2), 229-235.
- SYNTHESIS OF PHOSPHONATE ANALOGS OF 1,3-BISPHOSPHOGLYCERIC ACID AND THEIR BINDING TO YEAST PHOSPHOGLYCERATE KINASE. BIOORG MED CHEM LETT, 4(21), 2573-2578.
- Incorporation of bisphosphonates into adenine nucleotides by amoebae of the cellular slime mould Dictyostelium discoideum.. Biochem J, 303 ( Pt 1), 303-311.
- Polyphenols, astringency and proline-rich proteins.. Phytochemistry, 37(2), 357-371.
- Study of the interaction between salivary proline-rich proteins and a polyphenol by 1H-NMR spectroscopy.. Eur J Biochem, 219(3), 923-935.
- Nuclear magnetic resonance studies of peptides and their interactions with receptors.. Biochem Soc Trans, 22(1), 140-144.
- Conformational study of a salivary proline-rich protein repeat sequence.. Eur J Biochem, 219(3), 915-921.
- The structure and function of proline-rich regions in proteins.. Biochem J, 297 ( Pt 2), 249-260.
- STRUCTURE-ANALYSIS OF PROTEINS BY A COMBINATION OF DISTANCE GEOMETRY CALCULATION AND H-1-NMR CHEMICAL-SHIFT CALCULATION. KOBUNSHI RONBUNSHU, 51(6), 409-413.
- TURGOR REGULATION IN A NOVEL HALOMONAS SPECIES. ARCH MICROBIOL, 160(4), 319-323.
- NMR of proteins.. Nat Prod Rep, 10(3), 207-232.
- Mobility in pyruvate dehydrogenase complexes with multiple lipoyl domains.. FEBS Lett, 323(3), 243-246.
- EMPIRICAL COMPARISONS OF MODELS FOR CHEMICAL-SHIFT CALCULATION IN PROTEINS. J MAGN RESON SER B, 101(1), 63-71.
- Peptide structure determination by NMR.. Methods Mol Biol, 17, 69-85.
- Restructuring an interdomain linker in the dihydrolipoamide acetyltransferase component of the pyruvate dehydrogenase complex of Escherichia coli.. Protein Eng, 6(1), 101-108.
- A new approach to phosphoglycerate kinase: Synthetic, kinetic and NMR structural studies. ChemPlusChem, 58(s1), 109-112.
- PEPTIDE STRUCTURE FROM NMR. CHEM SOC REV, 21(4), 227-236.
- IMPROVED EXPERIMENTS FOR THE ASSIGNMENT OF CROWDED PEPTIDE SPECTRA. J MAGN RESON, 100(3), 593-597.
- Metabolism of halogenated bisphosphonates by the cellular slime mould Dictyostelium discoideum.. Biochem Biophys Res Commun, 189(1), 414-423.
- O-GLYCOSYLATION AND STABILITY - UNFOLDING OF GLUCOAMYLASE INDUCED BY HEAT AND GUANIDINE-HYDROCHLORIDE. EUR J BIOCHEM, 207(2), 661-670.
- DETERMINATION OF THE MAGNETIC-ANISOTROPY OF THE OXYGEN ATOM AND H-1 CHEMICAL-SHIFT CALCULATION OF PROTEINS. J MAGN RESON, 98(3), 646-653.
- The application of 1H NMR chemical shift calculations to diastereotopic groups in proteins.. FEBS Lett, 302(2), 185-188.
- O-GLYCOSYLATION IN ASPERGILLUS GLUCOAMYLASE - CONFORMATION AND ROLE IN BINDING. BIOCHEM J, 282, 423-428.
- A method for the calculation of protein alpha-CH chemical shifts.. J Biomol NMR, 2(1), 83-98.
- Refined crystal structure of Cd, Zn metallothionein at 2.0Åresolution. Journal of Molecular Biology, 221(4), 1269-1293.
- CALCULATION OF CHEMICAL-SHIFTS OF PROTONS ON ALPHA CARBONS IN PROTEINS. J MAGN RESON, 94(3), 557-562.
- NMR IN PROTEIN STUDIES. CHEM BRIT, 27(4), 335-337.
- 1H NMR studies on cyclo[-Arg(Mtr)-Gly-Asp(But)- Ser(But)-Lys(Boc)-] and cyclo(-Arg-Gly-Asp-Ser-Lys-), cyclic analogues of the 'adhesion' domain of fibronectin. Journal of the Chemical Society, Perkin Transactions 2(5), 601-606.
- Secondary-structure dependent chemical shifts in proteins.. Biopolymers, 29(10-11), 1423-1431.
- Rapid-pulsing artifacts in double-quantum-filtered COSY. Journal of Magnetic Resonance (1969), 88(1), 177-185.
- SECONDARY-STRUCTURE DEPENDENT CHEMICAL-SHIFTS IN PROTEINS. BIOPOLYMERS, 29(10-11), 1428-1431.
- Three-Dimensional Structure of Porcine C5a
desArg from 1H Nuclear Magnetic Resonance Data. Biochemistry, 29(12), 2895-2905.
- Measurement of cromakalim-induced 87Rb flux in intact cells by NMR. FEBS Letters, 254(1-2), 171-173.
- 1H-NMR and protection studies of interactions between ligands and bovine pancreatic phospholipase A2.. Biochim Biophys Acta, 997(1-2), 9-14.
- 1H nuclear magnetic resonance assignments and secondary structure of porcine C5ades Arg.. J Mol Biol, 206(2), 407-410.
- 1H nuclear magnetic resonance investigation of synovial fluid components in osteoarthritis, rheumatoid arthritis and traumatic effusions.. Br J Rheumatol, 28(1), 23-27.
- H-1 NUCLEAR MAGNETIC-RESONANCE INVESTIGATION OF SYNOVIAL-FLUID COMPONENTS IN OSTEO-ARTHRITIS, RHEUMATOID-ARTHRITIS AND TRAUMATIC EFFUSIONS. BRIT J RHEUMATOL, 28(1), 23-27.
- ChemInform Abstract: Structure Elucidation of a Glycopeptide Antibiotic, OA-7653.. ChemInform, 19(44).
- STRUCTURE ELUCIDATION OF A GLYCOPEPTIDE ANTIBIOTIC, OA-7653. J CHEM SOC PERK T 1(7), 1949-1956.
- ChemInform Abstract: Symmetry in NOE Spectra. ChemInform, 18(45).
- THE NUCLEAR OVERHAUSER EFFECT IN STRONGLY COUPLED SPIN SYSTEMS. J MAGN RESON, 73(1), 45-68.
- GUIDELINES FOR THE DESIGN OF KINETIC NOE EXPERIMENTS FROM COMPUTER-SIMULATION. MAGNETIC RESONANCE IN CHEMISTRY, 25(4), 356-361.
- SYMMETRY IN NOE SPECTRA. J MAGN RESON, 72(2), 369-375.
- SECONDARY STRUCTURE OF A HERPES-SIMPLEX VIRUS GLYCOPROTEIN-D ANTIGENIC DOMAIN. INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH, 27(5), 562-568.
- 1H‐NMR assignment and secondary structure of a Herpes simplex virus glycoprotein D‐1 antigenic domain. European Journal of Biochemistry, 158(3), 527-536.
- SOLUTION CONFORMATION OF PROTEINASE INHIBITOR-IIA FROM BULL SEMINAL PLASMA BY H-1 NUCLEAR MAGNETIC-RESONANCE AND DISTANCE GEOMETRY. J MOL BIOL, 182(2), 295-315.
- 1 H N.M.R. Studies of the structure of ristocetin a and of its complexes with bacterial cell wall analogues in aqueous solution. Journal of the Chemical Society, Perkin Transactions 1, 949-956.
- H-1-NMR STUDIES OF THE STRUCTURE OF RISTOCETIN-A AND OF ITS COMPLEXES WITH BACTERIAL-CELL WALL ANALOGS IN AQUEOUS-SOLUTION. J CHEM SOC PERK T 1(5), 949-956.
- STRUCTURAL FEATURES THAT AFFECT THE BINDING OF TEICOPLANIN, RISTOCETIN-A, AND THEIR DERIVATIVES TO THE BACTERIAL CELL-WALL MODEL N-ACETYL-D-ALANYL-D-ALANINE. J CHEM SOC CHEM COMM(5), 254-256.
- HYDROPHOBIC INTERACTIONS AFFECT HYDROGEN-BOND STRENGTHS IN COMPLEXES BETWEEN PEPTIDES AND VANCOMYCIN OR RISTOCETIN. EUR J BIOCHEM, 138(2), 345-348.
- A GLOBULAR PROTEIN WITH SLOWER AMIDE PROTON-EXCHANGE FROM AN ALPHA-HELIX THAN FROM ANTIPARALLEL BETA-SHEETS. BIOCHEM BIOPH RES CO, 122(3), 1174-1178.
- INTERACTIONS OF VANCOMYCIN AND RISTOCETIN WITH PEPTIDES AS A MODEL FOR PROTEIN-BINDING. TETRAHEDRON, 40(3), 569-577.
- SECONDARY STRUCTURE IN THE SOLUTION CONFORMATION OF THE PROTEINASE INHIBITOR-IIA FROM BULL SEMINAL PLASMA BY NUCLEAR MAGNETIC-RESONANCE. J MOL BIOL, 173(3), 341-359.
- PURE ABSORPTION PHASE PROTON 2D J-RESOLVED SPECTROSCOPY. J MAGN RESON, 55(3), 471-474.
- DETAILED BINDING-SITES OF THE ANTIBIOTICS VANCOMYCIN AND RISTOCETIN-A - DETERMINATION OF INTERMOLECULAR DISTANCES IN ANTIBIOTIC SUBSTRATE COMPLEXES BY USE OF THE TIME-DEPENDENT NOE. J AM CHEM SOC, 105(5), 1332-1339.
- STRUCTURE AND CONFORMATION OF 14 ANTIBIOTICS OF THE QUINOXALINE GROUP DETERMINED BY H-1-NMR. J ANTIBIOT, 35(1), 62-66.
- ON THE BIOSYNTHESIS OF THE ANTIBIOTIC VANCOMYCIN. J CHEM SOC CHEM COMM(6), 344-346.
- Erratum: Manipulation of the nuclear overhauser effect by the use of a viscous solvent: The solution conformation of the antibiotic echinomycin (Journal of the Chemical Society, Chemical Communications (1981) (165)). Journal of the Chemical Society, Chemical Communications(9), 440.
- MANIPULATION OF THE NUCLEAR OVERHAUSER EFFECT BY THE USE OF A VISCOUS SOLVENT - THE SOLUTION CONFORMATION OF THE ANTIBIOTIC ECHINOMYCIN. J CHEM SOC CHEM COMM(4), 165-166.
- STRUCTURE REVISION OF THE ANTIBIOTIC VANCOMYCIN - THE USE OF NUCLEAR OVERHAUSER EFFECT DIFFERENCE SPECTROSCOPY. J AM CHEM SOC, 103(22), 6580-6585.
- A C-13 NUCLEAR MAGNETIC-RESONANCE STUDY OF RISTOCETIN-A AND RISTOCETIN-B AND THEIR DERIVATIVES. J CHEM SOC PERK T 1(5), 1483-1491.
- ASSIGNMENT OF THE C-13 SPECTRUM OF VANCOMYCIN AND ITS DERIVATIVES. J CHEM SOC PERK T 2(1), 201-206.
- A C-13 NMR-STUDY OF THE CARBOHYDRATE PORTION OF RISTOCETIN-A. TETRAHEDRON LETT, 21(43), 4187-4188.
- STRUCTURE OF THE ANTIBIOTIC RISTOCETIN-A. J CHEM SOC CHEM COMM(20), 906-908.
- Interactions between the protein barnase and co-solutes studied by NMR. Communications Chemistry, 7(1).
-
DUF916 andDUF3324 in theWxL protein cluster bind toWxL and link bacterial and host surfaces. Protein Science.
- Quantitation of phosphohistidine in proteins in a mammalian cell line by 31P NMR. PLOS ONE, 17(9), e0273797-e0273797.
- CORM-3 induces DNA damage through Ru(II) binding to DNA. Biochemical Journal.
- ChemInform Abstract: Towards Stereochemical and Conformational Assignment in Flexible Molecules Using NOEs and Molecular Modelling.. ChemInform, 28(51), no-no.
- ChemInform Abstract: Peptide Structure from NMR. ChemInform, 24(12), no-no.
- ChemInform Abstract: NMR in Protein Studies. ChemInform, 22(32), no-no.
- 31P NMR Spectroscopy Demonstrates Large Amounts of Phosphohistidine in Mammalian Cells.
Chapters
- Nuclear magnetic resonance spectroscopy, Encyclopedia of Analytical Science (pp. 264-271).
- The Transferred NOE, Modern Magnetic Resonance (pp. 2133-2147). Springer International Publishing
- Chemical Shift Perturbation, Modern Magnetic Resonance (pp. 995-1012). Springer International Publishing
- Nuclear Magnetic Resonance Spectroscopy Techniques: Nuclear Overhauser Effect, Reference Module in Chemistry, Molecular Sciences and Chemical Engineering Elsevier
- The Transferred NOE, Modern Magnetic Resonance (pp. 1-15). Springer International Publishing
- Chemical Shift Perturbation, Modern Magnetic Resonance (pp. 1-19). Springer International Publishing
- NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY TECHNIQUES | Nuclear Overhauser Effect, Encyclopedia of Analytical Science (pp. 342-349). Elsevier
- NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY TECHNIQUES, Encyclopedia of Analytical Science (pp. V6-342-V6-349).
- Astringency and Polyphenol Protein Interactions, Phytochemicals in Human Health Protection, Nutrition, and Plant Defense (pp. 289-318). Springer US
- Solution Conformation of Proteinase Inhibitor IIA from Bull Seminal Plasma by
1
H
Nuclear Magnetic Resonance and Distance Geometry, NMR in Structural Biology (pp. 319-339).
- NOESY John Wiley & Sons, Ltd
- NOESY John Wiley & Sons, Ltd
- The Nuclear Overhauser Effect, Modern Magnetic Resonance (pp. 409-412). Springer Netherlands
- The Transferred NOE, Modern Magnetic Resonance (pp. 1357-1362). Springer Netherlands
Conference proceedings papers
- Epigallocatechin gallate, green tea catechin, binds to the T cell receptor, CD4. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, Vol. 117(2) (pp S325-S325)
- Binding of the green tea polyphenol, epigallocatechin gallate, to the CD4 receptor on human CD4+ T cells resulting in inhibition of HIV-1-gp120 binding. CLINICAL IMMUNOLOGY, Vol. 115 (pp S245-S246)
- Change in orientation and dynamics of DMPC molecules induced by aggregation of Aβ (1-40) molecules studied using solid state and solution NMR. Polymer Preprints, Japan, Vol. 54(2) (pp 4983)
- Structural and dynamical studies of Aβ(1-40)-Ganglioside system with solid state and solution NMR. Polymer Preprints, Japan, Vol. 54(1) (pp 727)
- Solving the first globular protein structure: BUSIIIA. MAGNETIC RESONANCE IN CHEMISTRY, Vol. 41 (pp S64-S69)
- Protein-polyphenol interactions. International Congress and Symposium Series - Royal Society of Medicine(226) (pp 25-33)
- Structural analysis of silk with C-13 NMR chemical shift contour plots. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, Vol. 24(2-3) (pp 167-171)
- How the N-terminal xylan-binding domain from C-fimi xylanase D recognises xylan. RECENT ADVANCES IN CARBOHYDRATE BIOENGINEERING(246) (pp 212-220)
- Salivary proteins as a defence against dietary tannins. COST 916 - POLYPHENOLS IN FOOD (pp 179-185)
- Salivary proline-rich proteins as a defence against dietary tannins. COST 916 - POLYPHENOLS IN FOOD (pp 201-201)
- AN ENZYME AND C-13-NMR STUDY OF CARBON METABOLISM IN HELIOBACTERIA. PHOTOSYNTHESIS RESEARCH, Vol. 41(1) (pp 75-88)
- Derivation of solution conformers of peptide hormones via constrained molecular dynamics based on 2-D NMR data. Proceedings of the Annual Conference on Engineering in Medicine and Biology(pt 4) (pp 1612)
Preprints
- The Enterococcal Polysaccharide Antigen: from structure to biosynthesis and function, Cold Spring Harbor Laboratory.
- Interactions between the protein barnase and co-solutes studied by NMR, Research Square Platform LLC.
- Improved methodology for protein NMR structure calculation using hydrogen bond restraints and ANSURR validation: the SH2 domain of SH2B1, Cold Spring Harbor Laboratory.
- The accuracy of protein structures in solution determined by AlphaFold and NMR, Cold Spring Harbor Laboratory.
- The accuracy of NMR protein structures in the Protein Data Bank, Cold Spring Harbor Laboratory.
- Decoration of the enterococcal polysaccharide antigen EPA is essential for virulence, cell surface charge and resistance to innate immunity, Cold Spring Harbor Laboratory.
- Autocatalytic selection as a driver for the origin of life. Life, 14(5). View this article in WRRO
- Research group
-
My laboratory uses NMR (and other methods where appropriate) to determine the structure and dynamics of proteins in solution and to study their interactions with ligands. In addition we are developing new methods for characterising structures. Further details are in my web page and in the publications list. Recent work includes:
We continue to study protein structures, particularly if this illustrates function. Recent targets include the PLAT domain of human polycystin-1, which we show to recognise phosphatidyl serine and PI4P in the membrane; the protein Mms6, which helps assemble magnetite particles in magnetotactic bacteria; and the Wbl protein (Figure), which uses an Fe-S cluster to sense NO in M. tuberculosis and hence evade host defences.
We have studied how proteins recognize polysaccharides such as starch, cellulose, xylan and peptidoglycan in bacterial cell walls: for example, the LysM module which recognises peptidoglycan and chitin, as found in bacterial and fungal cell walls and invertebrate exoskeletons.
We have been developing new tools; in particular the use of high hydrostatic pressure to stabilise partially unfolded structures, and thus investigate functional conformational change in proteins. We have started looking at Rheo-NMR, to see how proteins align and aggregate in laminar flow.
We collaborate with numerous groups. These include a logstanding collaboration with Tetsuo Asakura on silk structure; a collaboration with Jim Thomas on ruthenium-based complexes that bind B-DNA and quadruplexes; a collaboration with Robert Poole on the so-called Carbon Monoxide Releasing Molecules (CORMs); and a study on how Hofmeister ions stabilise and/or solubilise proteins.
We have a longstanding interest in polyphenols such as those from tea, and in how they interact with the body. As part of this study, we have shown that the main component of green tea, epigallocatechin gallate (EGCG), has the potential to slow down HIV infection; and that EGCG can be transported effectively by binding to albumin in the blood.
- Teaching activities
-
Level 4 modules
- MBB401 Introduction to research methodology
- MBB402 Advanced literature review
- MBB403 Extended laboratory project
Level 3 modules
- MBB334 Biochemical Basis of Human Disease (module coordinator) - amyloid disease, obesity and inflammation
- MBB343 Biochemical Signalling - principles, receptor tyrosine kinases, Notch and NF-kB
- MBB361 Literature review
- BIS303 Research project
Level 2 modules
- BIS206 Biostructures, Energetics and Synthesis – membranes, nerve transmission and signalling
- BIS220 Ethics and Philosophy for Molecular Biosciences
Level 1 modules
- MBB161 Biochemistry 1 – Orbitals and amino acids