Dr Lynda Partridge

School of Biosciences

Senior Lecturer

l.partridge@sheffield.ac.uk
+44 114 222 4185

Firth Court

Full contact details

Dr Lynda Partridge
School of Biosciences
Firth Court
Western Bank
Sheffield
S10 2TN

Profile

I am a Senior Lecturer in the School of Biosciences and teach Immunology to students in all years of our course. After a Biological Sciences degree at the University of Birmingham (specialising in Microbiology and Genetics) I carried out my PhD in Immunology at the Medical School there. I moved to Sheffield as a postdoctoral worker and later joined the Department as a lecturer. Most of my research now focusses on the tetraspanin family of membrane proteins and their role in infection, but I also have a long-standing interest in the application of antibody techniques to research and medicine. I’m currently Chair of the Yorkshire Branch of the Royal Society of Biology and am a Fellow of the Royal Society of Biology.

Career history

1979-1982: PhD, Department of Immunology, University of Birmingham
1882-1988: Post-doctoral researcher, Department of Biochemistry University of Sheffield
1988-1998: Lecturer, Department of Molecular Biology and Biotechnology, University of Sheffield
1990, 1993: Visiting Researcher, Scripps Research Clinic, La Jolla, California
2009-2013: Scientific Director, Bioserv UK Ltd.
1998-present: Senior Lecturer, School of Biosciences, University of Sheffield

Qualifications

PhD Immunology (University of Birmingham)
MSc Immunology (University of Birmingham)
BSc Biological Sciences (University of Birmingham)

Research interests

Tetraspanins in health and disease

Tetraspanins are a highly conserved superfamily of transmembrane proteins, with the first member having appeared some 570 million years ago! They are widely expressed in multicellular organisms and are involved in basic cell functions such as motility, fusion and membrane trafficking. Tetraspanins form promiscuous associations with one another and with other membrane proteins and lipids to generate a specialised type of microdomain: the tetraspanin enriched microdomain (TEM). There are 33 tetraspanins in mammals with roles in fertility, the immune response, cancer progression and susceptibility to infections.

Our group uses specific antibodies and other tetraspanin-based reagents to investigate how these proteins function in health and disease. We have shown that tetraspanins help control the formation of multinucleated giant cells (MNGCs), which are associated with chronic inflammation and certain types of infection, particularly those caused by viruses.

We have also discovered that many pathogenic bacteria “hijack” tetraspanin microdomains as a way of attaching to host cells before causing infection. This has encouraged us to develop tetraspanin-based reagents as alternatives to antibiotics for treating infections caused by a wide range of microbes such as Staphylococcus aureus (including MRSA).

Antibodies in research

I have also had a long-standing interest in the production and use of antibodies as tools in research and medicine. In the early 1990s, I helped establish a dedicated facility at the University for Custom Production of antibodies for researchers. A related spinout company, Bioserv UK Ltd, established in 2009, specialises in the production of antibodies and other proteins from mammalian cell culture.

Publications

Show: Featured publications All publications

Featured publications

Journal articles

Urwin L, Okurowska K, Crowther G, Roy S, Garg P, Karunakaran E, MacNeil S, Partridge LJ, Green LR & Monk PN (2020) Corneal infection models : tools to investigate the role of biofilms in bacterial keratitis. Cells, 9(11). View this article in WRRO
Sangsri T, Saiprom N, Tubsuwan A, Monk P, Partridge LJ & Chantratita N (2020) Tetraspanins are involved in Burkholderia pseudomallei-induced cell-to-cell fusion of phagocytic and non-phagocytic cells. Scientific Reports, 10. View this article in WRRO
Elgawidi A, Mohsin MI, Ali F, Watts A, Monk PN, Thomas MS & Partridge LJ (2020) A role for tetraspanin proteins in regulating fusion induced by Burkholderia thailandensis. Medical Microbiology and Immunology. View this article in WRRO
Elgawidi A, Mohsin MI, Ali F, Watts A, Monk PN, Thomas MS & Partridge LJ (2020) Correction to: A role for tetraspanin proteins in regulating fusion induced by Burkholderia thailandensis. Medical Microbiology and Immunology, 209(5), 643-652.
Hassuna NA, Monk PN, Ali F, Read RC & Partridge LJ (2017) A role for the tetraspanin proteins in Salmonella infection of human macrophages. Journal of Infection, 75(2), 115-124. View this article in WRRO
Ventress JK, Partridge LJ, Cozens D, Read RC, MacNeil S & Monk PN (2016) Peptides from Tetraspanin CD9 Are Potent Inhibitors of Staphylococcus Aureus Adherence to Keratinocytes. PLoS One, 11(7). View this article in WRRO
Rust A, Hassan HHA, Sedelnikova S, Niranjan D, Hautbergue G, Abbas SA, Partridge L, Rice D, Binz T & Davletov B (2015) Two complementary approaches for intracellular delivery of exogenous enzymes. Scientific Reports, 5, 12444-12444. View this article in WRRO
Hulme RS, Higginbottom A, Palmer J, Partridge LJ & Monk PN (2014) Distinct Regions of the Large Extracellular Domain of Tetraspanin CD9 Are Involved in the Control of Human Multinucleated Giant Cell Formation. PLoS ONE, 9(12). View this article in WRRO
Cruz-Migoni A, Hautbergue GM, Artymiuk PJ, Baker PJ, Bokori-Brown M, Chang C-T, Dickman MJ, Essex-Lopresti A, Harding SV, Mahadi NM , Marshall LE et al (2011) A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A.. Science, 334(6057), 821-824. View this article in WRRO
Green LR, Monk PN, Partridge LJ, Morris P, Gorringe AR & Read RC (2011) Cooperative role for tetraspanins in adhesin-mediated attachment of bacterial species to human epithelial cells.. Infect Immun, 79(6), 2241-2249. View this article in WRRO
Trikić MZ, Monk P, Roehl H & Partridge LJ (2011) Regulation of zebrafish hatching by tetraspanin cd63.. PLoS One, 6(5), e19683. View this article in WRRO
Parthasarathy V, Martin F, Higginbottom A, Murray H, Moseley GW, Read RC, Mal G, Hulme R, Monk PN & Partridge LJ (2009) Distinct roles for tetraspanins CD9, CD63 and CD81 in the formation of multinucleated giant cells.. Immunology, 127(2), 237-248.
Green LR, Issa R, Albaldi F, Urwin L, Thompson R, Khalid H, Turner CE, Ciani B, Partridge LJ & Monk PN () CD9 co-operation with syndecan-1 is required for a major staphylococcal adhesion pathway. mBio. View this article in WRRO
Rust A, Partridge L, Davletov B & Hautbergue G () The Use of Plant-Derived Ribosome Inactivating Proteins in Immunotoxin Development: Past, Present and Future Generations. Toxins, 9(11), 344-344. View this article in WRRO

All publications

Journal articles

Jadi PK, Dave A, Issa R, Tabassum K, Okurowska K, Samarth A, Urwin L, Green LR, Partridge LJ, MacNeil S , Garg P et al (2023) Tetraspanin CD9-derived peptides inhibits Pseudomonas aeruginosa corneal infection and aids in wound healing of corneal epithelial cells. The Ocular Surface.
Greaves S, Marsay KS, Monk PN, Roehl H & Partridge LJ (2022) Tetraspanin Cd9b plays a role in fertility in zebrafish. PLOS ONE, 17(11).
Marsay KS, Greaves S, Mahabaleshwar H, Ho CM, Roehl H, Monk PN, Carney TJ & Partridge LJ (2021) Tetraspanin Cd9b and Cxcl12a/Cxcr4b have a synergistic effect on the control of collective cell migration. PLoS ONE, 16(11). View this article in WRRO
Urwin L, Okurowska K, Crowther G, Roy S, Garg P, Karunakaran E, MacNeil S, Partridge LJ, Green LR & Monk PN (2020) Corneal infection models : tools to investigate the role of biofilms in bacterial keratitis. Cells, 9(11). View this article in WRRO
Sangsri T, Saiprom N, Tubsuwan A, Monk P, Partridge LJ & Chantratita N (2020) Tetraspanins are involved in Burkholderia pseudomallei-induced cell-to-cell fusion of phagocytic and non-phagocytic cells. Scientific Reports, 10. View this article in WRRO
Hassuna NA, Abdel-Malek MAY, Nasif Mohamed KA, Medhat M & Partridge LJ (2020) DAAs treatment is associated with up-regulation of CD81 on peripheral B-lymphocytes. Access Microbiology, 2(7A).
Emery K, Roy S, Thokala P, Partridge L, Garg P, MacNeil S, Monk P & Karunakaran E (2020) Establishing a porcine ex vivo cornea model for studying drug treatments against bacterial keratitis. Journal of Visualized Experiments. View this article in WRRO
Elgawidi A, Mohsin MI, Ali F, Watts A, Monk PN, Thomas MS & Partridge LJ (2020) A role for tetraspanin proteins in regulating fusion induced by Burkholderia thailandensis. Medical Microbiology and Immunology. View this article in WRRO
Elgawidi A, Mohsin MI, Ali F, Watts A, Monk PN, Thomas MS & Partridge LJ (2020) Correction to: A role for tetraspanin proteins in regulating fusion induced by Burkholderia thailandensis. Medical Microbiology and Immunology, 209(5), 643-652.
Dave A, Samarth A, Karolia R, Sharma S, Karunakaran E, Partridge L, MacNeil S, Monk P, Garg P & Roy S (2020) Characterization of ocular clinical isolates of Pseudomonas aeruginosa from non-contact lens related keratitis patients from south India. Microorganisms, 8(2). View this article in WRRO
Champion TC, Partridge LJ, Ong S-M, Malleret B, Wong S-C & Monk PN (2018) Monocyte Subsets Have Distinct Patterns of Tetraspanin Expression and Different Capacities to Form Multinucleate Giant Cells. Frontiers in Immunology, 9. View this article in WRRO
Hassuna NA, Monk PN, Ali F, Read RC & Partridge LJ (2017) A role for the tetraspanin proteins in Salmonella infection of human macrophages. Journal of Infection, 75(2), 115-124. View this article in WRRO
Yaseen IH, Monk PN & Partridge LJ (2017) Tspan2: a tetraspanin protein involved in oligodendrogenesis and cancer metastasis.. Biochem Soc Trans, 45(2), 465-475. View this article in WRRO
Ventress JK, Partridge LJ, Cozens D, Read RC, MacNeil S & Monk PN (2016) Peptides from Tetraspanin CD9 Are Potent Inhibitors of Staphylococcus Aureus Adherence to Keratinocytes. PLoS One, 11(7). View this article in WRRO
Logan A, Pell VR, Shaffer KJ, Evans C, Stanley NJ, Robb EL, Prime TA, Chouchani ET, Cochemé HM, Fearnley IM , Vidoni S et al (2016) Assessing the Mitochondrial Membrane Potential in Cells and In Vivo using Targeted Click Chemistry and Mass Spectrometry. Cell Metabolism, 23(2), 379-385.
Armanios M, de Cabo R, Mannick J, Partridge L, van Deursen J & Villeda S (2015) Translational strategies in aging and age-related disease. Nature Medicine, 21(12), 1395-1399.
Rust A, Hassan HHA, Sedelnikova S, Niranjan D, Hautbergue G, Abbas SA, Partridge L, Rice D, Binz T & Davletov B (2015) Two complementary approaches for intracellular delivery of exogenous enzymes. Scientific Reports, 5, 12444-12444. View this article in WRRO
Hulme RS, Higginbottom A, Palmer J, Partridge LJ & Monk PN (2014) Distinct Regions of the Large Extracellular Domain of Tetraspanin CD9 Are Involved in the Control of Human Multinucleated Giant Cell Formation. PLoS ONE, 9(12). View this article in WRRO
Partridge L (2014) Intervening in ageing to prevent the diseases of ageing. Trends in Endocrinology & Metabolism, 25(11), 555-557.
Piper MDW, Selman C, Speakman JR & Partridge L (2014) Using Doubly-Labeled Water to Measure Energy Expenditure in an Important Small Ectotherm Drosophila melanogaster. Journal of Genetics and Genomics, 41(9), 505-512.
Modi N, Vohra J, Preston J, Elliott C, Van't Hoff W, Coad J, Gibson F, Partridge L, Brierley J, Larcher V & Greenough A (2014) Guidance on clinical research involving infants, children and young people: an update for researchers and research ethics committees. Archives of Disease in Childhood, 99(10), 887-891.
Partridge L (2014) Editorial 2014. Philosophical Transactions of the Royal Society B: Biological Sciences, 369(1633), 20130496-20130496.
Niccoli T & Partridge L (2012) Ageing as a Risk Factor for Disease. Current Biology, 22(17), R741-R752.
Monk PN & Partridge LJ (2012) Tetraspanins: gateways for infection.. Infect Disord Drug Targets, 12(1), 4-17.
Cruz-Migoni A, Hautbergue GM, Artymiuk PJ, Baker PJ, Bokori-Brown M, Chang C-T, Dickman MJ, Essex-Lopresti A, Harding SV, Mahadi NM , Marshall LE et al (2011) A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A.. Science, 334(6057), 821-824. View this article in WRRO
Green LR, Monk PN, Partridge LJ, Morris P, Gorringe AR & Read RC (2011) Cooperative role for tetraspanins in adhesin-mediated attachment of bacterial species to human epithelial cells.. Infect Immun, 79(6), 2241-2249. View this article in WRRO
Fanaei M, Monk PN & Partridge LJ (2011) The role of tetraspanins in fusion.. Biochem Soc Trans, 39(2), 524-528.
Lee SF, Chen Y, Varan AK, Wee CW, Rako L, Axford JK, Good RT, Blacket MJ, Reuter C, Partridge L & Hoffmann AA (2011) Molecular Basis of Adaptive Shift in Body Size in Drosophila melanogaster: Functional and Sequence Analyses of the Dca Gene. Molecular Biology and Evolution, 28(8), 2393-2402.
Cochemé HM, Quin C, McQuaker SJ, Cabreiro F, Logan A, Prime TA, Abakumova I, Patel JV, Fearnley IM, James AM , Porteous CM et al (2011) Measurement of H2O2 within Living Drosophila during Aging Using a Ratiometric Mass Spectrometry Probe Targeted to the Mitochondrial Matrix. Cell Metabolism, 13(3), 340-350.
Alic N, Hoddinott MP, Vinti G & Partridge L (2011) Lifespan extension by increased expression of the Drosophila homologue of the IGFBP7 tumour suppressor. Aging Cell, 10(1), 137-147.
Partridge L, Thornton J & Bates G (2011) The new science of ageing. Philosophical Transactions of the Royal Society B: Biological Sciences, 366(1561), 6-8.
Trikić MZ, Monk P, Roehl H & Partridge LJ (2011) Regulation of zebrafish hatching by tetraspanin cd63.. PLoS One, 6(5), e19683. View this article in WRRO
Partridge L (2009) Some highlights of research on aging with invertebrates, 2009. Aging Cell, 8(5), 509-513.
Parthasarathy V, Martin F, Higginbottom A, Murray H, Moseley GW, Read RC, Mal G, Hulme R, Monk PN & Partridge LJ (2009) Distinct roles for tetraspanins CD9, CD63 and CD81 in the formation of multinucleated giant cells.. Immunology, 127(2), 237-248.
Hassuna N, Monk PN, Moseley GW & Partridge LJ (2009) Strategies for targeting tetraspanin proteins: potential therapeutic applications in microbial infections.. BioDrugs, 23(6), 341-359. View this article in WRRO
Milton CC & Partridge L (2008) Brief carbon dioxide exposure blocks heat hardening but not cold acclimation in Drosophila melanogaster. Journal of Insect Physiology, 54(1), 32-40.
Partridge L (2007) Some highlights of research on aging with invertebrates, 2006-2007. Aging Cell, 6(5), 595-598.
Bass TM, Weinkove D, Houthoofd K, Gems D & Partridge L (2007) Effects of resveratrol on lifespan in Drosophila melanogaster and Caenorhabditis elegans. Mechanisms of Ageing and Development, 128(10), 546-552.
Giannakou ME, Goss M, Jacobson J, Vinti G, Leevers SJ & Partridge L (2007) Dynamics of the action ofdFOXOon adult mortality inDrosophila. Aging Cell, 6(4), 429-438.
Wright AJ, Higginbottom A, Philippe D, Upadhyay A, Bagby S, Read RC, Monk PN & Partridge LJ (2007) Characterisation of receptor binding by the chemotaxis inhibitory protein of Staphylococcus aureus and the effects of the host immune response.. Mol Immunol, 44(10), 2507-2517.
Scola A-M, Higginbottom A, Partridge LJ, Reid RC, Woodruff T, Taylor SM, Fairlie DP & Monk PN (2007) The role of the N-terminal domain of the complement fragment receptor C5L2 in ligand binding.. J Biol Chem, 282(6), 3664-3671.
Ho SH, Martin F, Higginbottom A, Partridge LJ, Parthasarathy V, Moseley GW, Lopez P, Cheng-Mayer C & Monk PN (2006) Recombinant extracellular domains of tetraspanin proteins are potent inhibitors of the infection of macrophages by human immunodeficiency virus type 1. J VIROL, 80(13), 6487-6496.
Hamidpour M, Behrendt M, Griffiths B, Partridge L & Lindsey N (2006) The isolation and characterisation of antiplatelet antibodies. EUR J HAEMATOL, 76(4), 331-338.
Barnes AI, Boone JM, Jacobson J, Partridge L & Chapman T (2006) No extension of lifespan by ablation of germ line inDrosophila. Proceedings of the Royal Society B: Biological Sciences, 273(1589), 939-947.
Warner H, Anderson J, Austad S, Bergamini E, Bredesen D, Butler R, Carnes BA, Clark BFC, Cristofalo V, Faulkner J , Guarente L et al (2005) Science fact and the SENS agenda. EMBO reports, 6(11), 1006-1008.
Wertheim B, Kraaijeveld AR, Schuster E, Blanc E, Hopkins M, Pletcher SD, Strand MR, Partridge L & Godfray HCJ (2005) . Genome Biology, 6(11), R94-R94.
Martin F, Roth DM, Jans DA, Pouton CW, Partridge LJ, Monk PN & Moseley GW (2005) Tetraspanins in viral infections: a fundamental role in viral biology?. J Virol, 79(17), 10839-10851.
Muranova TA, Ruzheinikov SN, Higginbottom A, Clipson JA, Blackburn GM, Wentworth P, Datta A, Rice DW & Partridge LJ (2004) Crystallization of a carbamatase catalytic antibody Fab fragment and its complex with a transition-state analogue.. Acta Crystallogr D Biol Crystallogr, 60(Pt 1), 172-174.
Calboli FCF, Kennington WJ & Partridge L (2003) QTL MAPPING REVEALS A STRIKING COINCIDENCE IN THE POSITIONS OF GENOMIC REGIONS ASSOCIATED WITH ADAPTIVE VARIATION IN BODY SIZE IN PARALLEL CLINES OF DROSOPHILA MELANOGASTER ON DIFFERENT CONTINENTS. Evolution, 57(11), 2653-2658.
Calboli FCF, Gilchrist GW & Partridge L (2003) DIFFERENT CELL SIZE AND CELL NUMBER CONTRIBUTION IN TWO NEWLY ESTABLISHED AND ONE ANCIENT BODY SIZE CLINE OF DROSOPHILA SUBOBSCURA. Evolution, 57(3), 566-573.
Higginbottom A, Takahashi Y, Bolling L, Coonrod SA, White JM, Partridge LJ & Monk PN (2003) Structural requirements for the inhibitory action of the CD9 large extracellular domain in sperm/oocyte binding and fusion.. Biochem Biophys Res Commun, 311(1), 208-214.
Ruzheinikov SN, Muranova TA, Sedelnikova SE, Partridge LJ, Blackburn GM, Murray IA, Kakinuma H, Takahashi-Ando N, Shimazaki K, Sun J , Nishi Y et al (2003) High-resolution crystal structure of the Fab-fragments of a family of mouse catalytic antibodies with esterase activity.. J Mol Biol, 332(2), 423-435.
Moseley GW, Elliott J, Wright MD, Partridge LJ & Monk PN (2003) Interspecies contamination of the KM3 cell line: Implications for CD63 function in melanoma metastasis. INT J CANCER, 105(5), 613-616.
Cesaro-Tadic S, Lagos D, Honegger A, Rickard JH, Partridge LJ, Blackburn GM & Pluckthun A (2003) Turnover-based in vitro selection and evolution of biocatalysts from a fully synthetic antibody library. NAT BIOTECHNOL, 21(6), 679-685.
Behrendt M, Partridge LJ, Griffiths B, Goodfield M, Snaith M & Lindsey NJ (2003) The role of somatic mutation in determining the affinity of anti-DNA antibodies.. Clin Exp Immunol, 131(1), 182-189.
Carey JR, Liedo P, Harshman L, Zhang Y, Müller H-G, Partridge L & Wang J-L (2002) Life history response of Mediterranean fruit flies to dietary restriction. Aging Cell, 1(2), 140-148.
Gems D, Pletcher S & Partridge L (2002) Interpreting interactions between treatments that slow aging. Aging Cell, 1(1), 1-9.
Buckle C, Wells M & Partridge LJ (2002) Production of human monoclonal antibodies to an ovarian carcinoma-associated antigen (HPLAP) using phage display. J PATHOL, 198, 19A-19A.
Partridge L & Gems D (2002) Ageing: A lethal side-effect. Nature, 418(6901), 921-921.
Betley JR, Cesaro-Tadic S, Mekhalfia A, Rickard JH, Denham H, Partridge LJ, Plückthun A & Blackburn GM (2002) Erratum: (Angewandte Chemie - International Edition (2002) 5 (775-777)). Angewandte Chemie - International Edition, 41(9), 1460.
Betley JR, Cesaro-Tadic S, Mekhalfia A, Rickard JH, Denham H, Partridge LJ, Plückthun A & Blackburn GM (2002) Direkte Identifizierung von Proteinkatalysatoren mit Phosphatase-Aktivität durch kovalente Bindung an ein Suizid-Substrat. Angewandte Chemie, 114(5), 801-803.
Betley JR, Cesaro-Tadic S, Mekhalfia A, Rickard JH, Denham H, Partridge LJ, Plückthun A & Blackburn GM (2002) Direct screening for phosphatase activity by turnover-based capture of protein catalysts.. Angew Chem Int Ed Engl, 41(5), 775-777.
Gardner M, Fowler K, Partridge L & Barton N (2001) GENETIC VARIATION FOR PREADULT VIABILITY IN DROSOPHILA MELANOGASTER. Evolution, 55(8), 1609-1620.
Chapman T, Herndon LA, Heifetz Y, Partridge L & Wolfner MF (2001) The Acp26Aa seminal fluid protein is a modulator of early egg hatchability inDrosophila melanogaster. Proceedings of the Royal Society of London. Series B: Biological Sciences, 268(1477), 1647-1654.
Muranova TA, Ruzheinikov SN, Sedelnikova SE, Moir A, Partridge LJ, Kakinuma H, Takahashi N, Shimazaki K, Sun J, Nishi Y & Rice DW (2001) The preparation and crystallization of Fab fragments of a family of mouse esterolytic catalytic antibodies and their complexes with a transition-state analogue.. Acta Crystallogr D Biol Crystallogr, 57(Pt 8), 1192-1195.
Sgro CM, Geddes G, Fowler K & Partridge L (2000) SELECTION ON AGE AT REPRODUCTION IN DROSOPHILA MELANOGASTER: FEMALE MATING FREQUENCY AS A CORRELATED RESPONSE. Evolution, 54(6), 2152-2155.
Gilchrist AS & Partridge L (2000) WHY IT IS DIFFICULT TO MODEL SPERM DISPLACEMENT IN DROSOPHILA MELANOGASTER: THE RELATION BETWEEN SPERM TRANSFER AND COPULATION DURATION. Evolution, 54(2), 534-542.
Yazici ZA, Behrendt M, Cooper D, Goodfield M, Partridge LJ & Lindsey NJ (2000) The identification of endothelial cell autoantigens. J AUTOIMMUN, 15(1), 41-49.
BARTON NH & PARTRIDGE L (2000) Measuring fitness by means of balancer chromosomes. Genetical Research, 75(3), 297-313.
Higginbottom A, Wilkinson I, McCullough B, Lanza F, Azorsa DO, Partridge LJ & Monk PN (2000) Antibody cross-linking of human CD9 and the high-affinity immunoglobulin E receptor stimulates secretion from transfected rat basophilic leukaemia cells.. Immunology, 99(4), 546-552.
Zwaan BJ, Azevedo RBR, James AC, van 't Land J & Partridge L (2000) Cellular basis of wing size variation in Drosophila melanogaster: a comparison of latitudinal clines on two continents. Heredity, 84(3), 338-347.
Sgrò CM & Partridge L (1999) A Delayed Wave of Death from Reproduction in Drosophila. Science, 286(5449), 2521-2524.
Clayton R, Cooke ID, Partridge LJ & Moore HD (1998) A combinatorial phage display library for the generation of specific Fab fragments recognizing human spermatozoa and inhibiting fertilizing capacity in vitro.. Biol Reprod, 59(5), 1180-1186.
Herndon LA, Chapman T, Kalb JM, Lewin S, Partridge L & Wolfner MF (1997) Mating and hormonal triggers regulate accessory gland gene expression in male Drosophila. Journal of Insect Physiology, 43(12), 1117-1123.
Gilchrist AS & Partridge L (1997) Heritability of pre–adult viability differences can explain apparent heritability of sperm displacement ability inDrosophila melanogaster. Proceedings of the Royal Society of London. Series B: Biological Sciences, 264(1386), 1271-1275.
Azevedo RBR, French V & Partridge L (1997) Life‐History Consequences of Egg Size inDrosophila Melanogaster. The American Naturalist, 150(2), 250-282.
McCabe J & Partridge L (1997) An Interaction Between Environmental Temperature and Genetic Variation for Body Size for the Fitness of Adult Female Drosophila melanogaster. Evolution, 51(4), 1164-1164.
Gilchrist GW, Huey RB & Partridge L (1997) Thermal Sensitivity ofDrosophila melanogaster:Evolutionary Responses of Adults and Eggs to Laboratory Natural Selection at Different Temperatures. Physiological Zoology, 70(4), 403-414.
Yazici ZA, Beherendt M, Goodfield M, Partridge LJ & Lindsey NJ (1997) A human monoclonal antiendothelial cell autoantibody uses a V-H gene associated with an anti-DNA autoantibody.. BIOCHEM SOC T, 25(2), S321-S321.
Wentworth P, Datta A, Smith S, Marshall A, Partridge LJ & Blackburn GM (1997) Antibody catalysis of B(Ac)2 aryl carbamate ester hydrolysis: A highly disfavored chemical process. J AM CHEM SOC, 119(9), 2315-2316.
(1996) Female fitness inDrosophila melanogaster: an interaction between the effect of nutrition and of encounter rate with males. Proceedings of the Royal Society of London. Series B: Biological Sciences, 263(1371), 755-759.
Blackburn GM, Datta A & Partridge LJ (1996) The medical potential of catalytic antibodies. PURE APPL CHEM, 68(11), 2009-2016.
Yazici ZA, Beherendt M, Goodfield M, Partridge LJ & Lindsey NJ (1996) A human monoclonal antiendothelial cell autoantibody uses a V-H gene associated an anti-DNA autoantibody. IMMUNOLOGY, 89, BB321-BB321.
McCullough B, Monk P, Morton I & Partridge L (1996) hCD63 binding by MAb's from the platelet workshop blind panel. TISSUE ANTIGENS, 48(4-II), PL105-PL105.
McCullough B, Monk P, Morton I & Partridge L (1996) Analysis of the reactivity of antibodies from the blind panel of the myeloid workshop with hCD63. TISSUE ANTIGENS, 48(4-II), MC505-MC505.
Wentworth P, DAtta A, Blakey D, Boyle T, Partridge LJ & Blackburn GM (1996) Toward antibody-directed ''abzyme'' prodrug therapy, ADAPT: Carbamate prodrug activation by a catalytic antibody and its in vitro application to human tumor cell killing. P NATL ACAD SCI USA, 93(2), 799-803.
Smith DA, Monk PN & Partridge LJ (1995) Antibodies against human CD63 activate transfected rat basophilic leukemia (RBL-2H3) cells.. Mol Immunol, 32(17-18), 1339-1344.
Monk PN, Barker MD, Partridge LJ & Pease JE (1995) Mutation of glutamate 199 of the human C5a receptor defines a binding site for ligand distinct from the receptor N terminus.. J Biol Chem, 270(28), 16625-16629.
RYAN GJ, MULCAHY KA, MORTON K & PARTRIDGE LJ (1995) CHARACTERIZATION OF NUCLEAR TRANSPORT-SYSTEM AND MOLECULES INVOLVED IN NUCLEAR-PROTEIN LOCALIZATION IN HUMAN MONONUCLEAR PHAGOCYTE CELL-LINES. J CELL BIOCHEM, 145-145.
Monk PN, Barker MD & Partridge LJ (1994) Multiple signalling pathways in the C5a-induced expression of adhesion receptor Mac-1.. Biochim Biophys Acta, 1221(3), 323-329.
MOIR AJG, MCKENZIE A, BUDDRUS S, REED M & PARTRIDGE LJ (1994) DIGESTION OF ACTIN WITH GLY-C AND THE LOCALIZATION OF THE EPITOPE FOR THE MONOCLONAL-ANTIBODY NH3. J MUSCLE RES CELL M, 15(2), 196-196.
PARTRIDGE LJ, SHEN JQ, DENG SX, HEATON A, BORRIELLO A, BURTON DR & BLACKBURN GM (1994) STRATEGIES FOR THE PRODUCTION OF CATALYTIC ANTIBODIES USING COMBINATORIAL LIBRARIES. J CELL BIOCHEM, 195-195.
HEXHAM JM, PARTRIDGE LJ, FURMANIAK J, PETERSEN VB, COLLS JC, PEGG CAS, SMITH BR & BURTON DR (1994) HUMAN THYROID AUTOANTIBODIES FROM PCOMB3 PHAGE DISPLAY COMBINATORIAL LIBRARIES. J CELL BIOCHEM, 200-200.
Partridge LJ (1994) The production of monoclonal antibodies to membrane proteins.. Methods Mol Biol, 27, 65-86.
Hexham JM, Partridge LJ, Furmaniak J, Petersen VB, Colls JC, Pegg C, Rees Smith B & Burton DR (1994) Cloning and characterisation of TPO autoantibodies using combinatorial phage display libraries.. Autoimmunity, 17(3), 167-179.
Shen J, Deng SX, Partridge LJ & Blackburn GM (1993) New strategies for the generation of catalytic antibodies.. Biochem Soc Trans, 21(4), 415S.
Monk PN & Partridge LJ (1993) Characterization of a complement-fragment-C5a-stimulated calcium-influx mechanism in U937 monocytic cells.. Biochem J, 295 ( Pt 3), 679-684.
Partridge LJ (1993) Production of catalytic antibodies using combinatorial libraries.. Biochem Soc Trans, 21(4), 1096-1098.
WILLIAMSON RA, BURIONI R, SANNA PP, PARTRIDGE LJ, BARBAS CF & BURTON DR (1993) HUMAN MONOCLONAL-ANTIBODIES AGAINST A PLETHORA OF VIRAL PATHOGENS FROM SINGLE COMBINATORIAL LIBRARIES. P NATL ACAD SCI USA, 90(9), 4141-4145.
Santos M, Fowler K & Partridge L (1992) On the use of tester stocks to predict the competitive ability of genotypes. Heredity, 69(6), 489-495.
Partridge L (1992) Measuring reproductive costs. Trends in Ecology & Evolution, 7(3), 99-100.
Partridge LJ, Dransfield I & Burton D (1990) Characterization of a monoclonal antibody recognizing a novel leucocyte adhesion molecule.. Biochem Soc Trans, 18(2), 274-275.
Divecha N, Mansouri H, Peat D, Cope G, Partridge L & McDonald CJ (1989) Isoprenaline-induced and constitutive members of a proline-rich protein sub-group from mouse parotid glands studied with monoclonal antibody NAL1.. J Mol Endocrinol, 3(1), 7-14.
Burton DR, Jefferis R, Partridge LJ & Woof JM (1988) Molecular recognition of antibody (IgG) by cellular Fc receptor (FcRI).. Mol Immunol, 25(11), 1175-1181.
DUNCAN AR, WOOF JM, PARTRIDGE LJ, BURTON DR & WINTER G (1988) LOCALIZATION OF THE BINDING-SITE FOR THE HUMAN HIGH-AFFINITY FC RECEPTOR ON IGG. NATURE, 332(6164), 563-564.
DRANSFIELD I, MOIR AJG, SHETH B, BURTON DR & PARTRIDGE LJ (1988) INITIAL CHARACTERIZATION OF AN ANTI-ACTIN MONOCLONAL-ANTIBODY (NH3). BIOCHEM SOC T, 16(2), 163-164.
Sheth B, Dransfield I, Partridge LJ, Barker MD & Burton DR (1988) Dibutyryl cyclic AMP stimulation of a monocyte-like cell line, U937: a model for monocyte chemotaxis and Fc receptor-related functions.. Immunology, 63(3), 483-490.
Dransfield I, Corcoran D, Partridge LJ, Hogg N & Burton DR (1988) Comparison of human monocytes isolated by elutriation and adherence suggests that heterogeneity may reflect a continuum of maturation/activation states.. Immunology, 63(3), 491-498.
ALFADHL DH, SHARRARD RM, PARTRIDGE LJ, BURTON DR, GALLAGHER JA, SHEARD CE, BOBROW L, PRINGLE JAS & RUSSELL RGG (1987) CHARACTERIZATION OF A MONOCLONAL-ANTIBODY (DA1) WHICH REACTS WITH BONE-MATRIX AND BONE CELL CYTOPLASM. BONE, 8(1), 46-46.
PARTRIDGE LJ, WOOF JM, JEFFERIS R & BURTON DR (1986) THE USE OF ANTI-IGG MONOCLONAL-ANTIBODIES IN MAPPING THE MONOCYTE RECEPTOR-SITE ON IGG. MOL IMMUNOL, 23(12), 1365-1372.
WOOF JM, PARTRIDGE LJ, JEFFERIS R & BURTON DR (1986) LOCALIZATION OF THE MONOCYTE-BINDING REGION ON HUMAN IMMUNOGLOBULIN-G. MOL IMMUNOL, 23(3), 319-330.
PARTRIDGE L, JEFFERIS R, HARDIE D, LING NR & RICHARDSON P (1984) SUBCLASSES OF IGG ON THE SURFACE OF HUMAN-LYMPHOCYTES - A STUDY WITH MONOCLONAL-ANTIBODIES. CLIN EXP IMMUNOL, 56(1), 167-174.
PARTRIDGE LJ, LOWE J, HARDIE DL, LING NR & JEFFERIS R (1982) IMMUNOGENIC AND ANTIGENIC EPITOPES OF IMMUNOGLOBULINS .2. ANTIGENIC DIFFERENCES BETWEEN SECRETED AND MEMBRANE IGG DEMONSTRATED USING MONOCLONAL-ANTIBODIES. J IMMUNOL, 128(1), 1-6.
Green LR, Issa R, Albaldi F, Urwin L, Thompson R, Khalid H, Turner CE, Ciani B, Partridge LJ & Monk PN () CD9 co-operation with syndecan-1 is required for a major staphylococcal adhesion pathway. mBio. View this article in WRRO
Ma J, Scott CA, Ho YN, Mahabaleshwar H, Marsay KS, Zhang C, Teow CKJ, Ng SS, Zhang W, Tergaonkar V , Partridge LJ et al () Matriptase activation of Gq drives epithelial disruption and inflammation via RSK and DUOX. eLife, 10.
Partridge LJ, Urwin L, Nicklin MJH, James DC, Green LR & Monk PN () ACE2-Independent Interaction of SARS-CoV-2 Spike Protein with Human Epithelial Cells Is Inhibited by Unfractionated Heparin. Cells, 10(6), 1419-1419.
Okurowska K, Roy S, Thokala P, Partridge L, Garg P, MacNeil S, Monk PN & Karunakaran E () Establishing a Porcine Ex Vivo Cornea Model for Studying Drug Treatments against Bacterial Keratitis. Journal of Visualized Experiments(159).
Rust A, Partridge L, Davletov B & Hautbergue G () The Use of Plant-Derived Ribosome Inactivating Proteins in Immunotoxin Development: Past, Present and Future Generations. Toxins, 9(11), 344-344. View this article in WRRO
Sofola-Adesakin O, Castillo-Quan JI, Rallis C, Tain LS, Bjedov I, Rogers I, Li L, Martinez P, Khericha M, Cabecinha M , BÃ¤hler JR et al () Lithium suppresses AÎ² pathology by inhibiting translation in an adult Drosophila model of Alzheimer's disease. Frontiers in Aging Neuroscience, 6.
Metaxakis A, Tain LS, Grönke S, Hendrich O, Hinze Y, Birras U & Partridge L () Lowered Insulin Signalling Ameliorates Age-Related Sleep Fragmentation in Drosophila. PLoS Biology, 12(4), e1001824-e1001824.
Selman C, Partridge L & Withers DJ () Replication of Extended Lifespan Phenotype in Mice with Deletion of Insulin Receptor Substrate 1. PLoS ONE, 6(1), e16144-e16144.
Augustin H, Allen MJ & Partridge L () Electrophysiological Recordings from the Giant Fiber Pathway of <em>D. melanogaster</em>. Journal of Visualized Experiments(47).
Broughton S, Alic N, Slack C, Bass T, Ikeya T, Vinti G, Tommasi AM, Driege Y, Hafen E & Partridge L () Reduction of DILP2 in Drosophila Triages a Metabolic Phenotype from Lifespan Revealing Redundancy and Compensation among DILPs. PLoS ONE, 3(11), e3721-e3721.
Piper MDW & Partridge L () Dietary Restriction in Drosophila: Delayed Aging or Experimental Artefact?. PLoS Genetics, 3(4), e57-e57.
Mair W, Piper MDW & Partridge L () Calories Do Not Explain Extension of Life Span by Dietary Restriction in Drosophila. PLoS Biology, 3(7), e223-e223.

Conference proceedings papers

Hassuna N, Monk P, Read R & Partridge L (2010) The role of tetraspanin proteins in macrophage uptake of Salmonella bacteria. IMMUNOLOGY, Vol. 131 (pp 80-80)
Partridge LJ, Varadharajan P, Hulme R & Monk P (2008) Roles for tetraspanin proteins in the fusion of human monocytes to form multinucleated giant cells. IMMUNOLOGY, Vol. 125 (pp 6-7)
Scola AM, Higginbottom A, Partridge LJ & Monk PN (2007) Different ligand-binding mechanisms used by the two C5a receptors, C5L2 and C5aR. MOLECULAR IMMUNOLOGY, Vol. 44(1-3) (pp 237-237)
Blackburn GM, Rickard JH, Cesaro-Tadic S, Lagos D, Mekhalfia A, Partridge L & Pluckthun A (2004) Passive and catalytic antibodies and drug delivery. PURE AND APPLIED CHEMISTRY, Vol. 76(5) (pp 983-989)
Wong CH, Higginbottom A, Monk P, Partridge LJ & Moore HD (2001) Inhibition of sperm-oolemma interactions by the extracellular domains of CD9 and CD81. ANDROLOGY IN THE 21ST CENTURY, SHORT COMMUNICATIONS (pp 565-572)
Yazici ZA, Behrendt M, Goodfield M, Partridge LJ & Lindsey NJ (1998) Does the CDR3 of the heavy chain determine the specificity of autoantibodies in systemic lupus erythematosus?. JOURNAL OF AUTOIMMUNITY, Vol. 11(5) (pp 477-483)
McCullough B, Peppa D, Monk PN, Skubitz KM & Partridge LJ (1996) A role for CD63 in signal transduction. IMMUNOLOGY, Vol. 89 (pp OM114-OM114)
Higginbottom A, Partridge LJ & Monk PN (1996) Identification of potential ligands for CD63 from random peptide libraries. IMMUNOLOGY, Vol. 89 (pp OM117-OM117)

Preprints

Green LR, Issa R, Albaldi F, Urwin L, Khalid H, Turner CE, Ciani B, Partridge LJ & Monk PN (2023) CD9 co-operation with syndecan-1 is required for a major staphylococcal adhesion pathway, Cold Spring Harbor Laboratory.
Greaves S, Marsay KS, Monk PN, Roehl H & Partridge LJ () Tetraspanin Cd9b plays a role in fertility in zebrafish.
Marsay KS, Greaves S, Mahabaleshwar H, Ho CM, Roehl H, Monk PN, Carney TJ & Partridge LJ () Tetraspanin Cd9b and Cxcl12a/Cxcr4b have a synergistic effect on the control of collective cell migration.
Jiajia MA, Scott CA, Ying Na HO, Mahabaleshwar H, Marsay KS, Zhang C, Teow CKJ, Ser Sue NG, Zhang W, Tergaonkar V , Partridge LJ et al () Matriptase generates a tissue damage response via promoting Gq signalling, leading to RSK and DUOX activation, Cold Spring Harbor Laboratory.
Partridge LJ, Urwin L, Nicklin MJH, James DC, Green LR & Monk PN () ACE2-independent interaction of SARS-CoV-2 spike protein to human epithelial cells can be inhibited by unfractionated heparin.

Research group: Most of my current research is focussed on the role of tetraspanins in bacterial infections. Together with colleagues in Medicine and Engineering in Sheffield and at the LV-Prasad Eye Institute in Hyderabad, I am a co-investigator on an MRC GCRF-funded project that aims to develop new anti-adhesive treatments for infections that cause corneal ulceration and blindness. With colleagues in Medicine in Sheffield and Mahidol University in Bangkok, we have also been investigating the role of tetraspanins in the tropical disease melioidosis. I currently supervise three PhD students as primary supervisor and two as secondary supervisor.

Teaching activities

Level 4 modules

MBB405 Advanced Research Topics

Level 3 modules

MBB311 Molecular Immunology (Module Coordinator)

Level 2 modules

MBB263 Microbiology 2

Level 1 modules

MBB163 Microbiology

Professional activities and memberships: I am Chair of the Yorkshire Branch of Royal Society of Biology, represent the Sheffield region for the British Society of Immunology, and am a Fellow of the Royal Society of Biology.