Dr Indrajit Lahiri

School of Biosciences

Lecturer in Electron Microscopy

i.lahiri@sheffield.ac.uk
+44 114 222 20035

D14A, Firth Court

Full contact details

Dr Indrajit Lahiri
School of Biosciences
D14A
Firth Court
Western Bank
Sheffield
S10 2TN

Profile

2022 - present: Lecturer in Electron Microscopy, University of Sheffield

2019-2022: Assistant Professor, IISER Mohali, India

2015-2019: Postdoctoral Researcher, University of California, San Diego, USA

2014-2015: Postdoctoral Researcher, Harvard University, USA

2008-2014: PhD, State University of New York at Albany, USA

Research interests: We study the essential processes of genome replication, repair and compaction in eukaryotic parasites belonging to the phylum Apicomplexa.
Apicomplexans cause deadly diseases like malaria (Plasmodium sp.) and toxoplasmosis (Toxoplasma sp.). These parasites affect over 200 million people worldwide each year. Our interest lies in understanding how apicomplexans carry out DNA transactions within the context of their host - specifically how these processes occur in an organelle called the 'apicoplast'. To answer this question we use electron cryomicroscopy, fast enzyme kinetics, next-generation DNA sequencing and associated biochemical and biophysical techniques.

Publications

Sarsam RD, Xu J, Lahiri I, Gong W, Li Q, Oh J, Zhou Z, Hou P, Chong J, Hao N , Li S et al (2024) Elf1 promotes Rad26’s interaction with lesion-arrested Pol II for transcription-coupled repair. Proceedings of the National Academy of Sciences, 121(3), e2314245121.
Karasmanis EP, Reimer JM, Kendrick AA, Nguyen KHV, Rodriguez JA, Truong JB, Lahiri I, Reck-Peterson SL & Leschziner AE (2023) Lis1 relieves cytoplasmic dynein-1 autoinhibition by acting as a molecular wedge. Nature Structural & Molecular Biology, 30(9), 1357-1364. View this article in WRRO
Kumari A, Yadav A & Lahiri I (2022) Transient state kinetics of plasmodium falciparum apicoplast DNA polymerase suggests the involvement of accessory factors for efficient and accurate DNA synthesis. Biochemistry, 61(21), 2319-2333. View this article in WRRO
Bakker SE, Bergeron J, Bharadwaj A, Bhella D, Bullough P, Chau P-L, Frank RAW, Jakobi AJ, Joseph AP, Kühlbrandt W , Lahiri I et al () Map/model validation and machine learning in EM: general discussion. Faraday Discussions, 240, 229-242.

Stevenson SR, Tzokov SB, Lahiri I, Ayscough KR & Bullough PA (2024) CryoEM Reconstruction of Yeast ADP-Actin Filament at 2.5 Å resolution. A comparison with mammalian and avian F-actin, Cold Spring Harbor Laboratory.
Sarsam RD, Xu J, Lahiri I, Gong W, Oh J, Zhou Z, Chong J, Hao N, Li S, Wang D & Leschziner AE (2023) Elf1 promotes Rad26’s interaction with lesion-arrested Pol II for transcription-coupled repair, Cold Spring Harbor Laboratory.
Leschziner A, Karasmanis E, Reimer J, Kendrick A, Rodriguez J, Truong J, Lahiri I & Reck-Peterson S (2022) Lis1 relieves cytoplasmic dynein-1 autoinhibition by acting as a molecular wedge, Research Square Platform LLC.

Research group

Ms. Anamika Kumari (BBSRC White Rose DTP PhD student)

Dr. Anjali Yadav (Postdoctoral researcher)

School of Biosciences