Professor Per Bullough
School of Biosciences
Harrison Chair in Structural Biology
Harrison Chair in Structural Biology
p.bullough@sheffield.ac.uk
Firth Court
Full contact details
Professor Per Bullough
School of Biosciences
Firth Court
Western Bank
Sheffield
S10 2TN
School of Biosciences
Firth Court
Western Bank
Sheffield
S10 2TN
- Profile
-
Career history
- 2020 - present: Harrison Chair in Structural Biology, University of Sheffield
- 2001 - present: Professor, Dept. of Molecular Biology and Biotechnology, University of Sheffield
- 2002 - 2005: BBSRC Research Development Fellow
- 1995 - 2001: Lecturer, Dept. of Molecular Biology and Biotechnology, University of Sheffield
- 1994 - 1995: Staff Scientist, MRC Laboratory of Molecular Biology, Cambridge
- 1990 - 1994: Research Associate, Department of Biochemistry and Molecular Biology, Harvard University, USA
- 1989 - 1990: Research Associate, Howard Hughes Medical Institute, Harvard University, USA
- 1988 - 1989: Staff Scientist, MRC Laboratory of Molecular Biology, Cambridge
- 1985 - 1988: PhD, MRC Laboratory of Molecular Biology and Darwin College, University of Cambridge
- 1982 - 1985: B.Sc. Physics and Biology, King's College London
- Research interests
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- structural biology at cellular interfaces including membranes, cell walls and S-layers
- electron cryomicroscopy (CryoEM) and X-ray crystallography
- cellular architecture of pathogenic bacteria and spores including Clostridial and Bacillus species, C. difficile.
- atomic structure and mechanism of bacteriophages
- Publications
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Show: Featured publications All publications
Featured publications
Journal articles
- Molecular mechanism of bacteriophage contraction structure of an S-layer–penetrating bacteriophage. Life Science Alliance, 8(6). View this article in WRRO
- Cryo-EM reconstruction of yeast ADP-actin filament at 2.5 Å resolution. A comparison with vertebrate F-actin. Structure.
- Structure and assembly of the S-layer in C. difficile. Nature Communications, 13(1). View this article in WRRO
- Architecture and Self-Assembly of Clostridium sporogenes and Clostridium botulinum Spore Surfaces Illustrate a General Protective Strategy across Spore Formers. mSphere, 5(-), ---. View this article in WRRO
- The architecture of the Gram-positive bacterial cell wall. Nature, 582(7811), 294-297. View this article in WRRO
- Identification and structural analysis of the tripartite α-pore forming toxin of Aeromonas hydrophila. Nature Communications, 10(1), ---. View this article in WRRO
- Structural insights into the function of type VI secretion system TssA subunits.. Nature Communications, 9(-), ---. View this article in WRRO
- Molecular tiling on the surface of a bacterial spore – the exosporium of the Bacillus anthracis/cereus/thuringiensis group. Molecular Microbiology, 104(4), 539-552. View this article in WRRO
- Surface architecture of endospores of the Bacillus cereus/anthracis/thuringiensis family at the subnanometer scale.. Proc Natl Acad Sci U S A, 108(38), 16014-16019.
- The crystal structure of the Escherichia coli AmtB-GlnK complex reveals how GlnK regulates the ammonia channel.. Proc Natl Acad Sci U S A, 104(4), 1213-1218.
- The 8.5 A projection map of the light-harvesting complex I from Rhodospirillum rubrum reveals a ring composed of 16 subunits.. EMBO J, 14(4), 631-638.
- Structure of influenza haemagglutinin at the pH of membrane fusion.. Nature, 371(6492), 37-43.
All publications
Journal articles
- Molecular mechanism of bacteriophage contraction structure of an S-layer–penetrating bacteriophage. Life Science Alliance, 8(6). View this article in WRRO
- Cryo-EM reconstruction of yeast ADP-actin filament at 2.5 Å resolution. A comparison with vertebrate F-actin. Structure.
- Structure and assembly of the S-layer in C. difficile. Nature Communications, 13(1). View this article in WRRO
- Demonstration of the role of cell wall homeostasis in Staphylococcus aureus growth and the action of bactericidal antibiotics. Proceedings of the National Academy of Sciences, 118(44), e2106022118-e2106022118.
- Architecture and Self-Assembly of Clostridium sporogenes and Clostridium botulinum Spore Surfaces Illustrate a General Protective Strategy across Spore Formers. mSphere, 5(-), ---. View this article in WRRO
- The architecture of the Gram-positive bacterial cell wall. Nature, 582(7811), 294-297. View this article in WRRO
- Identification and structural analysis of the tripartite α-pore forming toxin of Aeromonas hydrophila. Nature Communications, 10(1), ---. View this article in WRRO
- The molecular basis of endolytic activity of a multidomain alginate lyase from Defluviitalea phaphyphila, a representative of a new lyase family, PL39. Journal of Biological Chemistry, 294(48), 18077-18091.
- Self‐Assembling Proteins as High‐Performance Substrates for Embryonic Stem Cell Self‐Renewal. Advanced Materials, 31(17), 1807521-1807521.
- Structural insights into the function of type VI secretion system TssA subunits.. Nature Communications, 9(-), ---. View this article in WRRO
- Molecular tiling on the surface of a bacterial spore – the exosporium of the Bacillus anthracis/cereus/thuringiensis group. Molecular Microbiology, 104(4), 539-552. View this article in WRRO
- Characterization of the spore surface and exosporium proteins of Clostridium sporogenes; implications for Clostridium botulinum group I strains. Food Microbiology, 59, 205-212. View this article in WRRO
- A Versatile Strategy for Production of Membrane Proteins with Diverse Topologies: Application to Investigation of Bacterial Homologues of Human Divalent Metal Ion and Nucleoside Transporters. PLOS ONE, 10(11), e0143010-e0143010. View this article in WRRO
- Diverse supramolecular structures formed by self-assembling proteins of the B acillus subtilis spore coat. Molecular Microbiology, 97(2), 347-359. View this article in WRRO
- Structural and functional consequences of removing the N-terminal domain from the magnesium chelatase ChlH subunit of Thermosynechococcus elongatus. Biochemical Journal, 464(3), 315-322. View this article in WRRO
- Structure and function of the bacterial heterodimeric ABC transporter CydDC: stimulation of ATPase activity by thiol and heme compounds.. J Biol Chem, 289(33), 23177-23188. View this article in WRRO
- Three-dimensional structure of the Rhodobacter sphaeroides RC-LH1-PufX complex: dimerization and quinone channels promoted by PufX.. Biochemistry, 52(43), 7575-7585.
- A urea channel from Bacillus cereus reveals a novel hexameric structure.. Biochem J, 445(2), 157-166.
- Structure of the cyanobacterial Magnesium Chelatase H subunit determined by single particle reconstruction and small-angle X-ray scattering.. J Biol Chem, 287(7), 4946-4956.
- AcrB contamination in 2-D crystallization of membrane proteins: lessons from a sodium channel and a putative monovalent cation/proton antiporter.. J Struct Biol, 176(3), 419-424.
- Surface architecture of endospores of the Bacillus cereus/anthracis/thuringiensis family at the subnanometer scale.. Proc Natl Acad Sci U S A, 108(38), 16014-16019.
- YwdL in Bacillus cereus: its role in germination and exosporium structure.. PLoS One, 6(8), e23801. View this article in WRRO
- AcrB contamination in 2-D crystallization of membrane proteins: Lessons from a sodium channel and a putative monovalent cation/proton antiporter. Journal of Structural Biology, 176(3), 419-424.
- Thermal and chemical unfolding and refolding of a eukaryotic sodium channel.. Biochim Biophys Acta, 1788(6), 1279-1286. View this article in WRRO
- Structure of the exosporium and sublayers of spores of the Bacillus cereus family revealed by electron crystallography.. Mol Microbiol, 68(4), 947-958.
- Colicin N binds to the periphery of its receptor and translocator, outer membrane protein F.. Structure, 16(3), 371-379.
- Three-dimensional reconstruction of a membrane-bending complex: the RC-LH1-PufX core dimer of Rhodobacter sphaeroides.. J Biol Chem, 283(20), 14002-14011.
- The formation and structure of Escherichia coli K-12 haemolysin E pores.. Microbiology, 154(Pt 2), 633-642.
- Single particle analysis of the dimeric RC-LH1-PufX complex from Rhodobacter sphaeroides. PHOTOSYNTH RES, 91(2-3), 171-171.
- The crystal structure of the Escherichia coli AmtB-GlnK complex reveals how GlnK regulates the ammonia channel.. Proc Natl Acad Sci U S A, 104(4), 1213-1218.
- Structure of the hemolysin E (HlyE, ClyA, and SheA) channel in its membrane-bound form.. J Biol Chem, 281(32), 23042-23049.
- The Escherichia coli AmtB protein as a model system for understanding ammonium transport by Amt and Rh proteins.. Transfus Clin Biol, 13(1-2), 97-102.
- Modelling the human rhesus proteins: implications for structure and function.. Br J Haematol, 131(4), 543-551.
- The crystal structures of Lactococcus lactis MG1363 Dps proteins reveal the presence of an N-terminal helix that is required for DNA binding.. Mol Microbiol, 57(4), 1101-1112.
- Obligate heterodimerization of the archaeal Alba2 protein with Alba1 provides a mechanism for control of DNA packaging.. Structure, 13(7), 963-971.
- The 8.5A projection structure of the core RC-LH1-PufX dimer of Rhodobacter sphaeroides.. J Mol Biol, 349(5), 948-960.
- Electron and atomic force microscopy of the trimeric ammonium transporter AmtB.. EMBO Rep, 5(12), 1153-1158.
- The native architecture of a photosynthetic membrane.. Nature, 430(7003), 1058-1062. View this article in WRRO
- Properties of haemolysin E (HlyE) from a pathogenic Escherichia coli avian isolate and studies of HlyE export.. Microbiology, 150(Pt 5), 1495-1505.
- Molecular architecture of photosynthetic membranes in Rhodobacter sphaeroides: the role of PufX.. EMBO J, 23(4), 690-700.
- Structural analysis of the reaction center light-harvesting complex I photosynthetic core complex of Rhodospirillum rubrum using atomic force microscopy.. J Biol Chem, 279(3), 2063-2068.
- Conformational flexibility in assembly of photosynthetic membrane protein complexes in vivo. Microscopy and Microanalysis, 10(SUPPL. 2), 1496-1497.
- Role of the C-terminal extrinsic region of the alpha polypeptide of the light-harvesting 2 complex of Rhodobacter sphaeroides: a domain swap study.. Biochemistry, 42(51), 15114-15123.
- A reaction center-light-harvesting 1 complex (RC-LH1) from a Rhodospirillum rubrum mutant with altered esterifying pigments: characterization by optical spectroscopy and cryo-electron microscopy.. J Biol Chem, 278(26), 23678-23685.
- The ATPase activity of the ChlI subunit of magnesium chelatase and formation of a heptameric AAA+ ring.. Biochemistry, 42(22), 6912-6920.
- Projection structure of the photosynthetic reaction centre-antenna complex of Rhodospirillum rubrum at 8.5 A resolution.. EMBO J, 21(15), 3927-3935.
- Glycerol dehydrogenase. structure, specificity, and mechanism of a family III polyol dehydrogenase.. Structure, 9(9), 789-802.
- Expression, refolding and crystallization of the OpcA invasin from Neisseria meningitidis.. Acta Crystallogr D Biol Crystallogr, 57(Pt 8), 1164-1166.
- The quarternary molecular architecture of TetA, a secondary tetracycline transporter from Escherichia coli.. Mol Microbiol, 38(3), 482-492.
- E. coli hemolysin E (HlyE, ClyA, SheA): X-ray crystal structure of the toxin and observation of membrane pores by electron microscopy.. Cell, 100(2), 265-276.
- Projection structure of reconstituted Opc outer membrane protein from Neisseria meningitidis. MOL MICROBIOL, 32(1), 217-219.
- The projection structure of the low temperature K intermediate of the bacteriorhodopsin photocycle determined by electron diffraction.. J Mol Biol, 286(5), 1663-1671.
- Protein Conformational Changes in the Bacteriorhodopsin Photocycle. Journal of Molecular Biology, 287, 145-161.
- Projection structures of three photosynthetic complexes from Rhodobacter sphaeroides: LH2 at 6 A, LH1 and RC-LH1 at 25 A.. J Mol Biol, 282(4), 833-845.
- Structural changes underlying proton pumping by Bacteriorhodopsin. FASEB J, 11(9), A992-A992.
- The 8.5 A projection map of the light-harvesting complex I from Rhodospirillum rubrum reveals a ring composed of 16 subunits.. EMBO J, 14(4), 631-638.
- Membrane fusion by influenza hemagglutinin.. Cold Spring Harb Symp Quant Biol, 60, 573-580.
- Crystals of a fragment of influenza haemagglutinin in the low pH induced conformation.. J Mol Biol, 236(4), 1262-1265.
- Structure of influenza haemagglutinin at the pH of membrane fusion.. Nature, 371(6492), 37-43.
- Spot-scan imaging of microcrystals of an influenza neuraminidase-antibody fragment complex.. Ultramicroscopy, 35(2), 131-143.
- High-resolution spot-scan electron microscopy of microcrystals of an alpha-helical coiled-coil protein.. J Mol Biol, 215(1), 161-173.
- Phase accuracy in high-resolution electron microscopy of trigonal and orthorhombic purple membrane.. Biophys J, 58(3), 705-711.
- Imaging of protein molecules--towards atomic resolution.. Electron Microsc Rev, 3(2), 249-267.
- USE OF SPOT-SCAN PROCEDURE FOR RECORDING LOW-DOSE MICROGRAPHS OF BEAM-SENSITIVE SPECIMENS. ULTRAMICROSCOPY, 21(3), 223-229.
- Tomographic analysis, CLEM: general discussion. Faraday Discussions, 240, 142-151.
- Pushing the limits in single particle cryo-EM: general discussion. Faraday Discussions.
- Sample preparation in single particle cryo-EM: general discussion. Faraday Discussions.
- Map/model validation and machine learning in EM: general discussion. Faraday Discussions.
- Correlative Super-Resolution Optical and Atomic Force Microscopy Reveals Relationships Between Bacterial Cell Wall Architecture and Synthesis in Bacillus subtilis. ACS Nano.
Chapters
- Refining a correlative light electron microscopy workflow using luminescent metal complexes, Methods in Cell Biology (pp. 69-87). Elsevier
- Electron Diffraction, Encyclopedia of Biophysics (pp. 581-586). Springer Berlin Heidelberg
- Reaction Center-Light-Harvesting Core Complexes of Purple Bacteria, The Purple Phototrophic Bacteria (pp. 155-179). Springer Netherlands
- 8.5 Å Projection Map of the Light-Harvesting Complex I from Rhodospirillum Rubrum Reveals a Ring Composed of 16 Subunits, Photosynthesis: from Light to Biosphere (pp. 81-84). Springer Netherlands
Conference proceedings papers
- The colicin N- OmpF translocation complex. BIOPHYSICAL JOURNAL, Vol. 88(1) (pp 50A-51A)
- Energy production in biological systems probed by electron crystallography. ELECTRON MICROSCOPY AND ANALYSIS 1999(161) (pp 127-132)
- Conformational changes in influenza haemagglutinin at the pH of membrane fusion. OPTIONS FOR THE CONTROL OF INFLUENZA III, Vol. 1123 (pp 307-314)
- 8.5 angstrom projection map of the light-harvesting complex I from Rhodospirillum rubrum reveals a ring composed of 16 subunits. PHOTOSYNTHESIS: FROM LIGHT TO BIOSPHERE, VOL I (pp 81-84)
- RECEPTOR BINDING AND MEMBRANE FUSION BY INFLUENZA HEMAGGLUTININ. CELLULAR RECEPTORS FOR ANIMAL VIRUSES (pp 187-193)
Datasets
Preprints
- CryoEM Reconstruction of Yeast ADP-Actin Filament at 2.5 Å resolution. A comparison with mammalian and avian F-actin, Cold Spring Harbor Laboratory.
- Molecular mechanism of bacteriophage tail contraction- structure of an S-layer-penetrating bacteriophage.
- Structure and assembly of the S-layer determine virulence in C. difficile. View this article in WRRO
- Architecture and self-assembly of theClostridium sporogenes/botulinumspore surface illustrate a general protective strategy across spore formers.
- Mitochondrial fission is increased in macrophages during mROS production in response to S. pneumoniae, Cold Spring Harbor Laboratory.
- Molecular mechanism of bacteriophage contraction structure of an S-layer–penetrating bacteriophage. Life Science Alliance, 8(6). View this article in WRRO
- Research group
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I welcome applications from prospective home / EU / overseas PhD students and post-doctoral fellows.
- Teaching activities
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Level 3 Modules
- MBB309 Membrane Protein Structure and Function (Module Coordinator)
- MBB302 Physical Methods for Studying Biological Structures
Level 1 Modules
- MBB162 Genetics