Biomedical Materials

On

Mauna Kea Technologies Cellvizio Dual Band

3D cellular and sub-cellular imaging of tissue. 

Detailed description

This equipment is a fluorescent-based Confocal Laser Endomicroscopy (CLE) system, bringing the confocal microprobe in contact with the tissue of interest to record realtime images of the tissue at cellular resolution, in vivo. The dual-colour capability (488nm and 660nm excitations) allows the capture of functional and morphological images of molecular mechanisms in real time. 

Detailed specifications

  • Simultaneous 488nm and 660nm. First line 502 - 633nm. Second line 673 - 800 nm.
  • Resolution: Between 3.3 and 1.4 μm
  • Frame rate: 9-18 fps
  • Advanced mode: 40 fps
  • Image: png, bmp, jpeg, tiff, mhd
  • Video: avi, mpeg, mp4, swf
  • Linux Ubuntu
  • Laser class: 3R
  • Height: 310mm
  • Width: 480mm
  • Depth: 525mm
  • Weight: 35kg
  • Cellvizio Lab Software 2.1.0.

Location

Kroto Research Institute, Red Hill, Sheffield City Centre, Sheffield, S3 7HQ
 

Zeiss Lightsheet Z.1

This equipment is used to image relatively large, living samples that are fluorescently labelled in some way. 

Detailed description

The equipment has incubation capabilities, so can be used with live samples (note that due to licensing limitations, this equipment cannot be used for imaging living organisms). There are five laser lines available with this instrument (405nm, 488nm, 514nm, 561nm, and 638nm) and the equipment is suitable for imaging whole samples up to approx 200 μm thick. 

The Lightsheet comes with three detection objectives (5x, 10x, and 20x) along with a zoom facility which allows between 0.3x and 2.5x magnification. As a result the equipment can achieve magnifications between 1.5x and 50x. 

Location

Kroto Research Institute, Red Hill, Sheffield City Centre, Sheffield, S3 7HQ

ENQUIRE HERE: royce@sheffield.ac.uk